miR-135a Targets and Regulates Prolactin Receptor Gene in Goat Mammary Epithelial Cells

Ji, Zhibin; Dong, Fei; Wang, Guizhi; Hou, Lei; Liu, Zhaohua; Chao, Tianle; Wang, Jianmin

doi:10.1089/dna.2015.2904

Cited by 19 publications

(12 citation statements)

References 31 publications

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“…For example, miR-135a expression level is associated with lactating stages and directly targets the prolactin receptor to function as a regulator in the goat mammary gland. 19 The expression level of miR-27a also was related to lactating stages and seems to play an important role in the regulation of TAG synthesis in GMEC. 31 There is evidence that miR-486 plays a key role in bovine mammary gland epithelial cells and promotes milk secretion.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-26a/b and their host genes synergistically regulate triacylglycerol synthesis by targeting theINSIG1gene

et al. 2016

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The microRNA-26 (miR-26) family is known to control adipogenesis in non-ruminants. The genomic loci of miR-26a and miR-26b have been localized in the introns of genes encoding for the proteins of the Cterminal domain RNA polymerase II polypeptide A small phosphatase (CTDSP) family. Insulin-induced gene 1 (INSIG1) encodes a protein with a key role in the regulation of lipogenesis in rodent liver. In the present study, we investigated the synergistic function of the miR-26 family and their host genes in goat mammary epithelial cells (GMEC). Downregulation of miR-26a/b and their host genes in GMEC decreased the expression of genes relate to fatty acid synthesis (PPARG, LXRA, SREBF1, FASN, ACACA, GPAM, LPIN1, DGAT1 and SCD1), triacylglycerol accumulation and unsaturated fatty acid synthesis. Luciferase reporter assays confirmed INSIG1 as a direct target of miR-26a/b. Furthermore, inhibition of the CTDSP family also downregulated the expression of INSIG1. Taken together, our findings highlight a functional association of miR-26a/b, their host genes and INSIG1, and provide new insights into the regulatory network controlling milk fat synthesis in GMEC. The data indicate that targeting this network via nutrition might be important for regulating milk fat synthesis in ruminants.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-26a/b and their host genes synergistically regulate triacylglycerol synthesis by targeting theINSIG1gene

et al. 2016

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“…Sass et al (2015) found that the functional roles of miRNA could be finished via functional annotation of target genes and identification of mRNA/miRNA interactions [29]. Ji et al (2015) showed that prolactin receptor is a target gene of miR-135a that may play a key role in regulating the development and lactation of caprine mammary gland [30]. MiR-143 is a crucial post-transcription regulator that is related to caprine mammary cell survival, and it may have an effect on the development, lactation or involution of mammary gland [31].…”

Section: Discussionmentioning

confidence: 99%

Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages

Hou

Song

et al. 2017

BMC Genet

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BackgroundMicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods.ResultThis study identified 131 differentially expressed miRNAs (P < 0.05 and log2 colostrum normalized expression (NE)/peak lactation NE > 1 or log2 colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and log2 colostrum NE/peak lactation NE > 1 or log2 colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway.ConclusionsOur finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-017-0498-2) contains supplementary material, which is available to authorized users.

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“…The roles of miRNAs in dairy lactation process or mammary gland development have been investigated using different approaches such as microarray3, RNA sequencing4567 and functional analyses of specific miRNAs891011121314. For instance, Wang et al 3.…”

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confidence: 99%

MicroRNA roles in signalling during lactation: an insight from differential expression, time course and pathway analyses of deep sequence data

Ngoc

Dudemaine

et al. 2017

Sci Rep

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The study examined microRNA (miRNA) expression and regulatory patterns during an entire bovine lactation cycle. Total RNA from milk fat samples collected at the lactogenesis (LAC, day1 [D1] and D7), galactopoiesis (GAL, D30, D70, D130, D170 and D230) and involution (INV, D290 and when milk production dropped to 5 kg/day) stages from 9 cows was used for miRNA sequencing. A total of 475 known and 238 novel miRNAs were identified. Fifteen abundantly expressed miRNAs across lactation stages play regulatory roles in basic metabolic, cellular and immunological functions. About 344, 366 and 209 miRNAs were significantly differentially expressed (DE) between GAL and LAC, INV and GAL, and INV and LAC stages, respectively. MiR-29b/miR-363 and miR-874/miR-6254 are important mediators for transition signals from LAC to GAL and from GAL to INV, respectively. Moreover, 58 miRNAs were dynamically DE in all lactation stages and 19 miRNAs were significantly time-dependently DE throughout lactation. Relevant signalling pathways for transition between lactation stages are involved in apoptosis (PTEN and SAPK/JNK), intracellular signalling (protein kinase A, TGF-β and ERK5), cell cycle regulation (STAT3), cytokines, hormones and growth factors (prolactin, growth hormone and glucocorticoid receptor). Overall, our data suggest diverse, temporal and physiological signal-dependent regulatory and mediator functions for miRNAs during lactation.

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miR-135a Targets and Regulates Prolactin Receptor Gene in Goat Mammary Epithelial Cells

Cited by 19 publications

References 31 publications

MicroRNA-26a/b and their host genes synergistically regulate triacylglycerol synthesis by targeting theINSIG1gene

MicroRNA-26a/b and their host genes synergistically regulate triacylglycerol synthesis by targeting theINSIG1gene

Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages

MicroRNA roles in signalling during lactation: an insight from differential expression, time course and pathway analyses of deep sequence data

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