miR-140-3p promotes follicle granulosa cell proliferation and steroid hormone synthesis via targeting AMH in chickens

Zhang, Beibei; Li, Xue-Nan; Li, Meng-Xiao; Sun, Yanyan; Shi, Yuxiang; Ma, Tenghe

doi:10.1016/j.theriogenology.2023.03.010

Cited by 9 publications

(3 citation statements)

References 36 publications

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“…For example, miR-429a-3p and miR-140-3p were overexpressed in ovary in at least five studied species. miR-429b-3p was reported to have lower expression in yellow catfish in YY super males compared to XY males (Jing et al, 2014) and in chicken, miR-140-3p was shown to promote germ cell proliferation while targeting anti-Müllerian hormone amh (Pfennig et al, 2015; Zhang et al, 2023). On the other hand, a male-like miRNA such as miR-184a-3p was shown to be a highly expressed miRNA in testis and involved in spermatogenesis in mice (Wu et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Identification of sex-biased miRNA markers informative of heat-past events

van Gelderen,

Montfort,

Álvarez-Dios

et al. 2024

Preprint

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Elevated temperatures during early developmental stages play a pivotal role in the fate of the final sexual phenotype of fish populations, particularly towards male-skewed sex ratios . This is the case with European sea bass (Dicentrarchus labrax), one of the most important European species in the aquaculture industry. To unveil informative markers of the past thermal events, we investigated changes in the miRNome within the gonads of this species. Consequently, we exposed European sea bass to elevated temperatures during early development and conducted miRNA-sequencing analysis in the ovaries and testes one year post-heat treatment. The examination of miRNA expression levels identified three and twelve miRNAs in ovaries and testes, respectively, reflecting past thermal events. To assess the evolutionary conservation of these identified miRNAs in gonads, we cross-referenced our data with miRNome public information from ovaries and testes in nine additional fish species. This analysis uncovered 43 potential sex-biased markers present in at least five studied species along the evolutionary timeline. For instance, miR-155, miR-429, and miR-140 were female-skewed while miR-184, miR-499, and miR-135 were male-skewed. In addition, among these markers, eight conserved sex-skewed miRNAs proved informative regarding past thermal events in both the ovaries (e.g., miR-192-5p and miR-143-3p) and testes (miR-129-5p, miR-2187a-3p, miR-724-5p, miR-143-3p, miR-194a-5p and miR-223-3p). Notably, miR-223-3p and miR-194a-5p were conserved female-skewed, but showed upregulation in males exposed to high temperature. These miRNAs could serve as markers of heat-induced masculinization of genetic female-prone fish. The current research broadens the inventory of sex-specific miRNAs along evolution in fish and, elucidates thermosensitive miRNAs in the gonads. These findings hold promise as potential tools for predicting historical environmental events associated with masculinization due to high-temperature treatments in cultured species but also perhaps for natural populations exposed to a climate change scenario.

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Section: Discussionmentioning

confidence: 99%

Identification of sex-biased miRNA markers informative of heat-past events

van Gelderen,

Montfort,

Álvarez-Dios

et al. 2024

Preprint

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“…A study of miRNAs in atretic follicles compared to healthy cattle follicles revealed that miR-150, miR-409a, miR-142-5p, miR-222, miR-155, and miR-199a-5p were more highly expressed in the theca than in the granulosa cells of atretic follicles [ 90 ]. In a rat POCS model induced by DHT, most of the miRNAs that stimulated cyst formation were expressed in follicular theca cells [ 91 , 92 ]. Hossain et al [ 93 ] reported the upregulation of miR-222 in theca cells of PCOS samples; however, androgens can target P27/kip1(a cyclin-CDK inhibitor) to regulate the proliferation of theca cells, leading to miR-222 suppression.…”

Section: Non-exosomal Mirna In Pcosmentioning

confidence: 99%

A glance into the roles of microRNAs (exosomal and non-exosomal) in polycystic ovary syndrome

Chelegahi,

Ebrahimi,

Reiisi

et al. 2024

Obstet Gynecol Sci

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Polycystic ovarian syndrome (PCOS) is a common endocrine disorder in women of reproductive age. The clinical symptoms include hyperandrogenism, chronic anovulation, and multiple ovarian cysts. PCOS is strongly associated with obesity and insulin resistance. MicroRNAs (miRNAs) are a group of short non-coding RNAs that play a role in the post-transcriptional regulation of gene expression and translational inhibition. They play a vital role in the regulation of multiple metabolic and hormonal processes as well as in oocyte maturation and folliculogenesis in the female reproductive system. miRNAs can be used as diagnostic biomarkers or therapeutic targets because of their stability. The encapsulation of miRNAs in extracellular vesicles or exosomes contributes to their stability. Exosomes are constantly secreted by many cells and size of about 30 to 150 nm. Enveloping miRNAs exosomes can release them for cellular communication. The induced transfer of miRNAs by exosomes is a novel process of genetic exchange between cells. Many studies have shown that along with non-exosomal miRNAs, different types of exosomal miRNAs derived from the serum and follicular fluid can play an essential role in PCOS pathogenesis. These miRNAs are involved in follicular development and various functions in granulosa cells, apoptosis, cell proliferation, and follicular atresia. The present study aimed to comprehensively review the evidence on miRNAs and their affected pathways under both non-exosomal and exosomal circumstances, primarily focusing on the pathogenesis of PCOS.

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“…Using chicken granulosa cells, AMH was identified as a direct target of miR-140–3p, which suppresses AMH expression. 71 Furthermore, lncRNA H19, which regulates miRNA-7 bioavailability, was identified to positively regulate ovarian AMH expression in mice. 72…”

Section: Regulation Of Ovarian Anti-müllerian Hormone Expressionmentioning

confidence: 99%

The Role of Anti-Müllerian Hormone in Ovarian Function

Yildiz,

Moolhuijsen,

Visser

2024

Semin Reprod Med

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Anti-Müllerian hormone (AMH) is a member of the transforming growth factor β (TGFβ) superfamily, whose actions are restricted to the endocrine-reproductive system. Initially known for its role in male sex differentiation, AMH plays a role in the ovary, acting as a gatekeeper in folliculogenesis by regulating the rate of recruitment and growth of follicles. In the ovary, AMH is predominantly expressed by granulosa cells of preantral and antral follicles (i.e., post primordial follicle recruitment and prior to follicle-stimulating hormone (FSH) selection). AMH signals through a BMP-like signaling pathway in a manner distinct from other TGFβ family members. In this review, the latest insights in AMH processing, signaling, its regulation of spatial and temporal expression pattern, and functioning in folliculogenesis are summarized. In addition, effects of AMH variants on ovarian function are reviewed.

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miR-140-3p promotes follicle granulosa cell proliferation and steroid hormone synthesis via targeting AMH in chickens

Cited by 9 publications

References 36 publications

Identification of sex-biased miRNA markers informative of heat-past events

Identification of sex-biased miRNA markers informative of heat-past events

A glance into the roles of microRNAs (exosomal and non-exosomal) in polycystic ovary syndrome

The Role of Anti-Müllerian Hormone in Ovarian Function

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