miR-140-5p inhibits the proliferation, migration and invasion of vascular smooth muscle cells by suppressing the expression of NCKAP1

Abstract

Introduction. The occurrence of aortic dissection is related to the proliferation and metastasis of vascular smooth muscle cells. In our present study, we found that the expression of miR-140-5p was inhibited in the wall of abdominal aorta of aortic dissection patients. However, the mechanism of miR-140-5p in the development of aortic dissection is unclear. Material and methods. We detected the expression of miR-140-5p and NCK Associated Protein 1 (NCKAP1) in blood vessel of aortic dissection patients and normal people by PCR. Next, we established the miR-140-5p overexpression and miR-140-5p inhibition vascular smooth muscle cells (CRL-1999 cells). The BrdU assays, wound healing assays and transwell assays were performed to detect the proliferation and invasion ability of these cells. Finally, luciferase reporter assay was performed to detect the relationship between miR-140-5p and NCKAP1. Results. The expression of miR-140-5p was suppressed in blood vessel of aortic dissection patients, and the levels of NCKAP1 in those tissues were upregulated. Overexpression of miR-140-5p inhibited the proliferation, migration and invasion of vascular smooth muscle cells. miR-140-5p targeted and suppressed the expression of NCKAP1. Conclusions. miR-140-5p repressed the proliferation, migration and invasion of vascular smooth muscle cells by targeting and inhibiting the expression of NCKAP1. Furthermore, the results of our study suggest new strategies and targets for the clinical treatment of arterial dissection.

A diagnostic model for atherosclerosis established on the basis of autophagy-related genes

“…A study revealed that miR-140-5p-mediated inhibition of NCKAP1 leads to a decrease in the ability of cells to proliferate, migrate, and invade. This could be due to the suppression of NCKAP1 affecting the cell's skeletal structure and signal transduction pathways, thus in uencing the cell's ability to proliferate, migrate, and invade [15]. Additionally, NCKAP1 is closely associated with several molecular pathways (the in ammatory promotion pathway, HLA pathway, cell lytic activity, cell apoptosis pathway, type II IFN response, complement and coagulation cascade responses) and several cytokines (PDGFRB, IL-10, IFNG, and TNF-α).…”

Integrative bioinformatics analysis of miRNA and mRNA expression profiles and identification of associated miRNA-mRNA network in intracranial aneurysms

“…Previous studies have shed light on the significance of miR-188-5p in AAA progression, demonstrating its upregulation and its impact on elastin degradation, VSMC depletion, and mural angiogenesis inhibition [ 34 ]. Similarly, miR-140-5p suppression has been observed in acute aortic dissection (AAD) patients, correlating with upregulated NCKAP1 levels, influencing VSMC proliferation, migration, and invasion [ 35 ]. Deng et al, investigated the role of miR-140/BCL2L2 axis on the formation of IAs.…”

miR‐140 and miR‐200 regulate the migratory heterogeneity of location‐specific Schwann cell population

“…Biological activities of miR‐140 and miR‐200 in Schwann cells remain largely undetermined although an earlier study demonstrates that miR‐140 directly interacts with myelin transcription factor Egr2 (Krox‐20) and suppresses Schwann cell myelination (Viader et al, 2011). Actually, miR‐140 has been identified as a migration suppressor in many cell types, including macrophages, vascular smooth muscle cells, breast cancer cells, and hepatocellular carcinoma cells (Dou et al, 2021; Li et al, 2021; Ma et al, 2021; Qiao et al, 2022). By restraining the EMT process, miR‐140 hinders cell migration and invasion (Guo et al, 2020).…”

“…Therefore, (Viader et al, 2011). Actually, miR-140 has been identified as a migration suppressor in many cell types, including macrophages, vascular smooth muscle cells, breast cancer cells, and hepatocellular carcinoma cells (Dou et al, 2021;Li et al, 2021;Ma et al, 2021;Qiao et al, 2022). By restraining the EMT process, miR-140 hinders cell migration and invasion (Guo et al, 2020).…”