miR‐140‐5p‐mediated regulation of the proliferation and differentiation of human dental pulp stem cells occurs through the lipopolysaccharide/toll‐like receptor 4 signaling pathway

Abstract

Human dental pulp stem cells (DPSCs) are oral mesenchymal stem cells with potential to differentiate into various cell types. Recent studies of DPSCs have focused on microRNAs (miRNAs), a class of small noncoding RNAs that play crucial roles in regulating DPSC phenotypes. In the current study, the expression of miR-140-5p was significantly decreased during lipopolysaccharide (LPS)-mediated differentiation of DPSCs in vitro. Overexpression of miR-140-5p enhanced proliferation of DPSCs and inhibited DPSC differentiation, whereas suppression of miR-140-5p produced the opposite effect. Moreover, the expression of toll-like receptor 4 (TLR-4), a critical regulator of DPSCs, was negatively correlated with the levels of miR-140-5p. A luciferase reporter analysis confirmed that miR-140-5p could regulate TLR-4 by directly binding to the 3'-untranslated region (3'-UTR) of the TLR4 mRNA. Additionally, we suppressed TLR-4 expression by treating cells with a TLR-4 inhibitor, CLI-095, and demonstrated that the effect of the miR-140-5p inhibitor on DPSC proliferation and differentiation could be partially reversed by blocking TLR-4. Taken together, our data suggest that miR-140-5p is a novel miRNA that regulates DPSC proliferation and differentiation.

Genome-wide identification of long noncoding RNAs and their competing endogenous RNA networks involved in the odontogenic differentiation of human dental pulp stem cells

“…In the research on ncRNAs and their roles in the odontogenic differentiation of hDPSCs, the most studied are miRNAs. miR-223-3p and miR-21 have been shown to promote odontogenic differentiation, while miR-143-5p, miR-140-5p, and miR-488 inhibit this process [41][42][43][44][45][46]. In 2016, Chen et al found that lncRNA differentiation antagonizing non-protein coding RNA (DANCR) inhibited the odontogenic differentiation of human dental pulp cells by inactivating the Wnt/β-catenin signaling pathway [16].…”

LncRNA H19 Promotes Odontoblastic Differentiation of Human Dental Pulp Stem Cells by Regulating miR-140-5p and BMP-2/FGF9

“…al. [35] found that miR-140-5p-mediated regulation of the proliferation and differentiation of human dental pulp stem cells occurs through the lipopolysaccharide/toll-like receptor 4 signaling pathway. However, the downstream target genes regulated by H19/mir-140-5p axis remains unclear.…”

Expression of microRNA miR-27, miR-124 and miR-218 Among Dental Pulp Stem Cell (DPSC) Isolates

“…These results are significant as the evidence for microRNA expression among DPSC isolates is in the very early stages of exploration and few studies to date have evaluated this phenomenon (Tu et al, 2018;Sun et al, 2017;Li et al, 2015). This study screened for miR-143 and miR-135 expression, which was demonstrated to function in the pathway for myogenic differentiation of DPSC (Li et al, 2015), although virtually no information is currently available about the normal function and expression of these microRNA among non-differentiated DPSC.…”

“…MicroRNAs are small non-coding RNA molecules that can act as transcriptional activators and repressors in many types of mesenchymal stem cells (Katsuda and Ochiya, 2015;Utikal et al, 2015;Huang et al, 2016;Martin et al, 2016) Some microRNAs (miR), such as miR-21 and miR-16 appear to be significant biomarkers and modulators of MSC potential and differentiation (Sekar et al, 2015;Clark et al, 2014;Fakhry et al, 2013). To date, few studies have evaluated the expression or activity of microRNAs among dental pulp stem cells (Tu et al, 2018;Sun et al, 2017;Li et al, 2015).…”