MiR-140-5p/TLR4 /NF-κB signaling pathway: Crucial role in inflammatory response in 16HBE cells induced by dust fall PM2.5

Chen, Xiangwa; Deng, Tao; Huo, Tingting; Dong, Faqin; Deng, Jianjun

doi:10.1016/j.ecoenv.2020.111414

Cited by 18 publications

(14 citation statements)

References 54 publications

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“…Chen et al have measured the composition of PM 2.5 , which was also used in this study, and confirmed that PM 2.5 could induce inflammatory response in 16HBE cells via the TLR4/NF‐κB signaling pathway, and the possible catalyst is the LPS present in PM 2.5 . This further verified that dust fall PM 2.5 used in this study can induce acute inflammatory injury in rat lungs 23 . However, in the present study, pretreatment of vitamin B complex, especially in medium and high concentrations, could alleviate inflammatory and lung injury to a certain extent.…”

Section: Discussionsupporting

confidence: 84%

“…PM 2.5 was provided by the Key Laboratory of Solid Waste Treatment and Resource Recycle, Ministry of Education Southwest University of Science and Technology in Mianyang, Sichuan Province of China 23 . Detached PM 2.5 were vacuum‐freeze dried, weighed and stored at −20°C.…”

Section: Methodsmentioning

confidence: 99%

“…The DNA methylation level of IL-4 and IFN-γ gene promoter, the level of genomic methylation rate (5-mC%), the ratio of SAM/S-Adenosyl-L-homocysteine (SAH) and the mRNA and protein expression of DNMTs were determined to explain the role of DNA methylation in the process. 23 Detached PM 2.5 were vacuum-freeze dried, weighed and stored at À20 C. To establish the rat model, a suspension of PM 2.5 with a concentration of 2.0 mg/ml was prepared in sterile saline solution. The suspended PM 2.5 was sonicated for 30 min before intratracheal instillation.…”

mentioning

confidence: 99%

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Vitamin B complex blocks the dust fall PM₂_.5‐induced acute lung injury through DNA methylation in rats

Bai

Tang

Luo

et al. 2022

Environmental Toxicology

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This study aimed to explore whether vitamin B complex (folic acid, B 6 , and B 12 ) could avert DNA methylation changes associated with inflammation induced by acute PM 2.5 exposure. Sprague-Dawley rats were administered by gavage with different concentrations of vitamin B complex once a day for 28 days, and then by intratracheal instillation with saline or PM 2.5 once every 2 days for three times. Vitamin B continued to be taken during the PM 2.5 exposure. Rats were sacrificed 24 h after the last exposure. The results showed that vitamin B complex could block the pathological changes and injury in lungs induced by PM 2.5 . Meanwhile, vitamin B complex could prevent the abnormal DNA methylation of IL-4 and IFN-γ to antagonize the imbalance of IL-4/IFN-γ associated with inflammation. It was further found that vitamin B complex could regulate DNA methyltransferases (DNMTs) and increase the Sadenosylmethionine (SAM)/S-Adenosyl-L-homocysteine (SAH) ratio to reverse the hypomethylation of genomic DNA and the abnormal DNA methylation of IL-4 and IFN-γ. In conclusion, vitamin B complex has a protective effect on acute lung injury by attenuating abnormal DNA methylation induced by PM 2.5 in rats. This study may provide a new insight into the physiological function of vitamin B to prevent the health effects induced by PM 2.5 .

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Section: Discussionsupporting

confidence: 84%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Vitamin B complex blocks the dust fall PM₂_.5‐induced acute lung injury through DNA methylation in rats

Bai

Tang

Luo

et al. 2022

Environmental Toxicology

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“…Emerging studies also showed that low miR-140 levels is closely related to elevated MALAT1 and PAK1 levels [ 38 ]. Functionally, miR-140 not only targets oncogene loci but could also be involved in the TLR4/NF-κB axis and KCNQ1OT1/miR-140-5p/SOX4 axis [ 39 , 40 ]. Although miR-140 is widely recognized as a tumor suppressor, the suggestion that miR-140 is an oncogenic agent continues to emerge.…”

Section: Discussionmentioning

confidence: 99%

Prognostic and clinicopathological importance of microRNA-140 expression in cancer patients: a meta-analysis

Zheng

Liu²,

Meng

et al. 2021

World J Surg Onc

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Background MicroRNA-140 (miR-140) is one of the most widely investigated miRNAs in cell carcinogenesis and cancer development. Despite present proposals of employing miR-140 as a candidate biomarker for cancer prognosis, its effectiveness in predicting patient survival and clinicopathological outcome is still under debate. Methods A systematic search for English literature using online databases was performed with pre-established criteria. Odds ratios (ORs) or hazard ratios (HRs) with 95% confidence intervals (CIs) were collected to delineate the correlation between miR-140 levels and cancer patient prognosis. Results For this meta-analysis, we selected 12 papers for analysis, involving 1386 participants. Based on our analysis, high levels of miR-140 were strongly correlated with enhanced patient overall survival (OS) (HR = 0.728, 95% CI = 0.601-0.882, P = 0.001). In addition, we also observed that elevated miR-140 levels significantly led to better OS in patients with cancers in different parts of the body like digestive system (HR = 0.675, 95% CI = 0.538-0.848, P = 0.001), digestive tract (HR = 0.709, 95% CI = 0.565-0.889, P = 0.003), and head and neck (HR = 0.603, 95% CI = 0.456-0.797, P < 0.001). Additionally, we verified that the low miR-140 levels was related to advanced TNM stage (OR = 0.420, 95% CI = 0.299-0.590, P < 0.001), worse histologic grade (OR = 0.410, 95% CI = 0.261-0.643, P < 0.001), and positive lymph node metastasis status (OR = 0.341, 95% CI = 0.144-0.807, P = 0.014). Conclusions Taken together, our results suggest that elevated miR-140 levels can be employed as a favorable biomarker for cancer patient prognosis. This information can greatly benefit in the formation of an individualized therapeutic plan for the treatment of cancer patients.

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“…There were some studies of mir-140-5p were reported, such as mir-140-5p directly targeted Nrf2 and Sirt2 and thereby increasing DOX-caused myocardial oxidative damage, mir-140-5p negatively regulated TLR4 to mediate inflammation in 16HBE cells and mir-140-5p suppressed activation of the Wnt/β-catenin and NF-κB pathways by targeting SOX4. Thus, mir-140-5p could affect some signaling pathways through target genes ( 51 – 53 ). This study preliminarily analyzed the correlation of miRNA and mRNA after CDV infected Mv.…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of miRNA-mRNA Expression in Mink Lung Epithelial Cells Infected With Canine Distemper Virus

et al. 2022

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Canine distemper (CD) caused by canine distemper virus (CDV) is one of the major infectious diseases in minks, bringing serious economic losses to the mink breeding industry. By an integrated analysis of microRNA (miRNA)-messenger RNA (mRNA), the present study analyzed the changes in the mink transcriptome upon CDV infection in mink lung epithelial cells (Mv. l. Lu cells) for the first time. A total of 4,734 differentially expressed mRNAs (2,691 upregulated and 2,043 downregulated) with |log2(FoldChange) |>1 and P-adj<0.05 and 181 differentially expressed miRNAs (152 upregulated and 29 downregulated) with |log2(FoldChange) |>2 and P-adj<0.05 were identified. Gene Ontology (GO) enrichment indicated that differentially expressed genes (DEGs) were associated with various biological processes and molecular function, such as response to stimulus, cell communication, signaling, cytokine activity, transmembrane signaling receptor activity and signaling receptor activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the combination of miRNA and mRNA was done for immune and inflammatory responses, such as Janus kinase (JAK)-signal transducer and activator (STAT) signaling pathway and nuclear factor (NF)-kappa B signaling pathway. The enrichment analysis of target mRNA of differentially expressed miRNA revealed that mir-140-5p and mir-378-12 targeted corresponding genes to regulate NF-kappa B signaling pathway. JAK-STAT signaling pathway could be modulated by mir-425-2, mir-139-4, mir-140-6, mir-145-3, mir-140-5p and mir-204-2. This study compared the influence of miRNA-mRNA expression in Mv. l. Lu cells before and after CDV infection by integrated analysis of miRNA-mRNA and analyzed the complex network interaction between virus and host cells. The results can help understand the molecular mechanism of the natural immune response induced by CDV infection in host cells.

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MiR-140-5p/TLR4 /NF-κB signaling pathway: Crucial role in inflammatory response in 16HBE cells induced by dust fall PM2.5

Cited by 18 publications

References 54 publications

Vitamin B complex blocks the dust fall PM₂_.5‐induced acute lung injury through DNA methylation in rats

Vitamin B complex blocks the dust fall PM₂_.5‐induced acute lung injury through DNA methylation in rats

Prognostic and clinicopathological importance of microRNA-140 expression in cancer patients: a meta-analysis

Integrated Analysis of miRNA-mRNA Expression in Mink Lung Epithelial Cells Infected With Canine Distemper Virus

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MiR-140-5p/TLR4 /NF-κB signaling pathway: Crucial role in inflammatory response in 16HBE cells induced by dust fall PM2.5

Cited by 18 publications

References 54 publications

Vitamin B complex blocks the dust fall PM2.5‐induced acute lung injury through DNA methylation in rats

Vitamin B complex blocks the dust fall PM2.5‐induced acute lung injury through DNA methylation in rats

Prognostic and clinicopathological importance of microRNA-140 expression in cancer patients: a meta-analysis

Integrated Analysis of miRNA-mRNA Expression in Mink Lung Epithelial Cells Infected With Canine Distemper Virus

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Vitamin B complex blocks the dust fall PM₂_.5‐induced acute lung injury through DNA methylation in rats

Vitamin B complex blocks the dust fall PM₂_.5‐induced acute lung injury through DNA methylation in rats