miR-142-3p Expression Is Predictive for Severe Traumatic Brain Injury (TBI) in Trauma Patients

Abstract

Background: Predictive biomarkers in biofluids are the most commonly used diagnostic method, but established markers in trauma diagnostics lack accuracy. This study investigates promising microRNAs (miRNA) released from affected tissue after severe trauma that have predictive values for the effects of the injury. Methods: A retrospective analysis of prospectively collected data and blood samples of n = 33 trauma patients (ISS ≥ 16) is provided. Levels of miR-9-5p, -124-3p, -142-3p, -219a-5p, -338-3p and -423-3p in severely injured patients (PT) without traumatic brain injury (TBI) or with severe TBI (PT + TBI) and patients with isolated TBI (isTBI) were measured within 6 h after trauma. Results: The highest miR-423-3p expression was detected in patients with severe isTBI, followed by patients with PT + TBI, and lowest levels were found in PT patients without TBI (2−∆∆Ct, p = 0.009). A positive correlation between miR-423-3p level and increasing AIShead (p = 0.001) and risk of mortality (RISC II, p = 0.062) in trauma patients (n = 33) was found. ROC analysis of miR-423-3p levels revealed them as statistically significant to predict the severity of brain injury in trauma patients (p = 0.006). miR-124-3p was only found in patients with severe TBI, miR-338-3p was shown in all trauma groups. miR-9-5p, miR-142-3p and miR-219a-5p could not be detected in any of the four groups. Conclusion: miR-423-3p expression is significantly elevated after isolated traumatic brain injury and predictable for severe TBI in the first hours after trauma. miR-423-3p could represent a promising new biomarker to identify severe isolated TBI.

MiR-124-3p Suppresses the Dysfunction of High Glucose-Stimulated Endothelial Cells by Targeting G3BP2

“…Consistent with this study, previous research has shown that miR-124-3p restrains cell injury in some other diseases, for example, miR-124-3p alleviates the neuronal injury of SH-SY5Y cells induced by MPP + (Geng et al, 2017). MiR-124-3p inhibits cell injury in traumatic brain injury (Vuokila et al, 2018;Schindler et al, 2020). MiR-124-3p alleviates neuronal apoptosis induced by mechanical injury (Su et al, 2019).…”

“…MiR-1273g-3p is involved in DR development (Ye et al, 2017). Recently, it has been reported that miR-124-3p participates in the regulation of various diseases, such as traumatic brain injury (Schindler et al, 2020) and neuropathic pain (Zhang et al, 2019). Importantly, miR-124-3p was reported to be associated with DR (You et al, 2018).…”

Influence of Antibiotic Management on Microbial Selection and Infectious Complications After Trauma

“…The standard clinical diagnosis of pneumonia includes fever, leukocytosis, purulent secretions, and new or progressive infiltrates in a chest x-ray (32). Nevertheless, biomarkers are certainly the optimal complement to clinical and radiological findings to assess the course and outcome of the injury and its complications sufficiently (10,33). We could show that levels of IL-6 and CRP are significantly higher in trauma patients who develop post-traumatic infection.…”

MicroRNA-124: A Key Player in Microglia-Mediated Inflammation in Neurological Diseases

“…miR-124-3p levels were elevated at 2 days post-TBI in both the blood and plasma of patients; an elevated plasma miR-124-3p level 2 days post-TBI was positively correlated with larger lesion area at the chronic time point in TBI models ( Vuokila et al, 2020 ). Furthermore, miR-124-3p was detected only in patients with severe TBI ( Schindler et al, 2020 ). The miR-124-3p level was elevated in the serum of patients with TBI ( O’connell et al, 2020 ).…”