miR‑142‑5p promotes renal cell tumorigenesis by targeting TFAP2B

Zhu, Maoshu; Zou, Liangneng; Lu, Fuhua; Ye, Ling; Su, Bin; Yang, Kai-Chun; Lin, Minghua; Fu, Jianqian; Li, Yongwu

doi:10.3892/ol.2020.12187

Cited by 8 publications

(6 citation statements)

References 23 publications

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“…Other miRNAs targeted genes involved in cell–cell interaction, cell death, cell survival and cellular assembly and organization network including miR-142-5p, miR-151-3p, miR-218-1-3p, miR-335, and miR-340 related with AGO2, AGO3, CDH1, GABRA3 ( Figure 3 C). These results are similar to previous reports in renal cell carcinoma [ 34 ] and thyroid cancer [ 35 ]. The results of network analysis are presented in Figure 3 .…”

Section: Resultssupporting

confidence: 93%

Differentially Regulated miRNAs and Their Related Molecular Pathways in Lichen Sclerosus

Tan

Ren

Yang

et al. 2021

Cells

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Lichen sclerosus (LS) is a chronic inflammatory skin disorder with unknown pathogenesis. The aberrant expression of microRNAs (miRNAs) is considered to exert a crucial role in LS. We used the next-generation sequencing technology (RNASeq) for miRNA profiling and Ingenuity Pathway Analysis (IPA) for molecular network analysis. We performed qRT-PCR, miRNA transfection and Matrigel assays for functional studies. We identified a total of 170 differentially expressed miRNAs between female LS and matched adjacent normal tissue using RNASeq, with 119 upregulated and 51 downregulated. Bioinformatics analysis revealed molecular networks that may shed light on the pathogenesis of LS. We verified the expression of a set of miRNAs that are related to autoimmunity, such as upregulated miR-326, miR-142-5p, miR-155 and downregulated miR-664a-3p and miR-181a-3p in LS tissue compared to the matched adjacent normal tissue. The differentially expressed miRNAs were also verified in blood samples from LS patients compared to healthy female volunteers. Functional studies demonstrated that a forced expression of miR-142-5p in human dermal fibroblast PCS-201-010 cells resulted in decreased cell proliferation and migration. These findings suggest that differentially expressed miRNAs may play an important role in LS pathogenesis; therefore, they could serve as biomarkers for LS management.

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Section: Resultssupporting

confidence: 93%

Differentially Regulated miRNAs and Their Related Molecular Pathways in Lichen Sclerosus

Tan

Ren

Yang

et al. 2021

Cells

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“…42 Over the years, there have been a growing body of studies that underscores the role of miR-142-5p in pathophysiological development of many diseases, such as epilepticus, 43 diabetic retinopathy, 44 nonalcoholic fatty liver disease, 45 and cancers. 46 Studies concerning the role of miR-142-5p in cardiovascular diseases is still lacking, but there is some evidence. Xu et al 47 found that miR-142-5p level is significantly increased in the atherosclerotic plaque obtained from ApoE −/− mice.…”

Section: Discussionmentioning

confidence: 99%

Gut Microbially Produced Indole-3-Propionic Acid Inhibits Atherosclerosis by Promoting Reverse Cholesterol Transport and Its Deficiency Is Causally Related to Atherosclerotic Cardiovascular Disease

Xue

Chen

et al. 2022

Circulation Research

106

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BACKGROUND: Accumulating evidence has shown that disorders in the gut microbiota and derived metabolites affect the development of atherosclerotic cardiovascular disease (ASCVD). However, which and how specific gut microbial metabolites contribute to the progression of atherosclerosis and the clinical relevance of their alterations remain unclear. METHODS: We performed integrated microbiome–metabolome analysis of 30 patients with coronary artery disease (CAD) and 30 age- and sex-matched healthy controls to identify CAD-associated microbial metabolites, which were then assessed in an independent population of patients with ASCVD and controls (n=256). We further investigate the effect of CAD-associated microbial metabolites on atherosclerosis and the mechanisms of the action. RESULTS: Indole-3-propionic acid (IPA), a solely microbially derived tryptophan metabolite, was the most downregulated metabolite in patients with CAD. Circulating IPA was then shown in an independent population to be associated with risk of prevalent ASCVD and correlated with the ASCVD severity. Dietary IPA supplementation alleviates atherosclerotic plaque development in ApoE −/− mice. In murine- and human-derived macrophages, administration of IPA promoted cholesterol efflux from macrophages to ApoA-I through an undescribed miR-142-5p/ABCA1 (ATP-binding cassette transporter A1) signaling pathway. Further in vivo studies demonstrated that IPA facilitates macrophage reverse cholesterol transport, correlating with the regulation of miR-142-5p/ABCA1 pathway, whereas reduced IPA production contributed to the aberrant overexpression of miR-142-5p in macrophages and accelerated the progression of atherosclerosis. Moreover, the miR-142-5p/ABCA1/reverse cholesterol transport axis in macrophages were dysregulated in patients with CAD, and correlated with the changes in circulating IPA levels. CONCLUSIONS: Our study identify a previously unknown link between specific gut microbiota-derived tryptophan metabolite and ASCVD. The microbial metabolite IPA/miR-142-5p/ABCA1 pathway may represent a promising therapeutic target for ASCVD.

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“…Numerous studies have highlighted the essential roles of TFAP2B, not only in the various tissues affected by the Char syndrome, but also in the neural processes, tumorigenesis, and craniofacial development (Fu et al, 2014; Fu et al, 2019; Hu & Bringmann, 2023; Ikram et al, 2016; Satoda et al, 2000; Zhu et al, 2020). Our lineage tracing has revealed that Tfap2b+ cells are progenitors for tooth development.…”

Section: Discussionmentioning

confidence: 99%

Generation of tamoxifen‐inducible Tfap2b‐CreER^T2 mice using CRISPR‐Cas9

Zhang,

Feng,

et al. 2023

Genesis

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SummaryTfap2b, a pivotal transcription factor, plays critical roles within neural crest cells and their derived lineage. To unravel the intricate lineage dynamics and contribution of these Tfap2b+ cells during craniofacial development, we established a Tfap2b‐CreERT2 knock‐in transgenic mouse line using the CRISPR‐Cas9‐mediated homologous direct repair. By breeding with tdTomato reporter mice and initiating Cre activity through tamoxifen induction at distinct developmental time points, we show the Tfap2b lineage within the key neural crest‐derived domains, such as the facial mesenchyme, midbrain, cerebellum, spinal cord, and limbs. Notably, the migratory neurons stemming from the dorsal root ganglia are visible subsequent to Cre activity initiated at E8.5. Intriguingly, Tfap2b+ cells, serving as the progenitors for limb development, show activity predominantly commencing at E10.5. Across the mouse craniofacial landscape, Tfap2b exhibits a widespread presence throughout the facial organs. Here we validate its role as a marker of progenitors in tooth development and have confirmed that this process initiates from E12.5. Our study not only validates the Tfap2b‐CreERT2 transgenic line, but also provides a powerful tool for lineage tracing and genetic targeting of Tfap2b‐expressing cells and their progenitor in a temporally and spatially regulated manner during the intricate process of development and organogenesis.

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miR‑142‑5p promotes renal cell tumorigenesis by targeting TFAP2B

Cited by 8 publications

References 23 publications

Differentially Regulated miRNAs and Their Related Molecular Pathways in Lichen Sclerosus

Differentially Regulated miRNAs and Their Related Molecular Pathways in Lichen Sclerosus

Gut Microbially Produced Indole-3-Propionic Acid Inhibits Atherosclerosis by Promoting Reverse Cholesterol Transport and Its Deficiency Is Causally Related to Atherosclerotic Cardiovascular Disease

Generation of tamoxifen‐inducible Tfap2b‐CreER^T2 mice using CRISPR‐Cas9

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miR‑142‑5p promotes renal cell tumorigenesis by targeting TFAP2B

Cited by 8 publications

References 23 publications

Differentially Regulated miRNAs and Their Related Molecular Pathways in Lichen Sclerosus

Differentially Regulated miRNAs and Their Related Molecular Pathways in Lichen Sclerosus

Gut Microbially Produced Indole-3-Propionic Acid Inhibits Atherosclerosis by Promoting Reverse Cholesterol Transport and Its Deficiency Is Causally Related to Atherosclerotic Cardiovascular Disease

Generation of tamoxifen‐inducible Tfap2b‐CreERT2 mice using CRISPR‐Cas9

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Generation of tamoxifen‐inducible Tfap2b‐CreER^T2 mice using CRISPR‐Cas9