MiR-142-5p promotes retinoblastoma cell proliferation, migration and invasion by targeting PTEN

Abstract

The study intends to probe the functions of miR-142-5p in retinoblastoma (RB) and the relationship between miR-142-5p and phosphatase and tensin homolog deleted on chromosome ten (PTEN). In our study, miR-142-5p and PTEN mRNA expression in RB tissue, serum of RB patients and RB cell lines were investigated by quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation, migration, invasion and cell apoptosis were measured using MTT assay, BrdU assay, Transwell experiments and flow cytometry analysis respectively. Binding sites between miR-142-5p and PTEN were predicted by the TargetScan database and were confirmed via qRT-PCR, Western blot, and dual-luciferase reporter gene assay. It was demonstrated that miR-142-5p expression was elevated in RB tissue, serum of RB patients, and RB cell lines. MiR-142-5p overexpression remarkably promoted the proliferation, migration, invasion and inhibited the apoptosis of WERI-RB-1 cells while miR-142-5p knockdown induced opposite effects in Y79 cells. MiR-142-5p decreased PTEN expression in both mRNA and protein expression levels, and PTEN was identified as a target gene of miR-142-5p. Co-transfection of PTEN overexpression plasmids reversed the influences of miR-142-5p on RB cells. In conclusion, miR-142-5p enhances proliferation, migration and invasion of RB cell by targeting PTEN.

MiR-19a-3p/PTEN axis regulates the anticancer effect of circHIAT1 in breast cancer <i>in vitro</i>

“…Our data also displayed that the miR-19a-3p/PTEN axis modulates the invasive behavior of breast cancer, while miR-19a-3p inhibitor suppressed the invasion of breast cancer cells, and PTEN silencing antagonized the effect of miR-19a-3p inhibitor. This finding is consistent with the previous report that PTEN functions as a negative regulator of cell migration and invasion (Dayoub et al, 2022;Zhang and Zheng, 2021). Since PTEN is also found to regulate the epithelial-mesenchymal transition (EMT) in cancer (Fedorova et al, 2022), it remains to be determined whether the miR-19a-3p/PTEN axis modulates the EMT status of breast cancer.…”

Potential Role of Selected miRNAs in the Pathogenesis of Autoimmune Thyroid Diseases in Children and Adolescents

“…The miR-142-5p molecule also presents high specificity for hematopoietic cells and increased levels are observed in many pathological conditions such as inflammation [18], immune disorders [19] and cancers, for example, retinoblastoma [20] and breast cancer [21]. Moreover, the downregulation of miR-142-5p may serve as an additional indicator of metastasis in thyroid cancer [22].…”