miR-143 is critical for the formation of primordial follicles in mice

Zhang, Jianfang; Ji, Xiaowen; Zhou, Doudou; Li, Yueqin; Lin, Jun-Kai; Liu, Jiali; Luo, Hanpeng; Cui, Sheng

doi:10.2741/4122

Cited by 45 publications

(7 citation statements)

References 36 publications

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“…Most recently, microRNA expression profiles have been established in ovaries in human (Zhang et al 2011), mice (Choi et al 2007, Ro et al 2007, Ahn et al 2010, Zhang et al 2013, and cattle (Tripurani et al 2010 around the time of primordial follicle assembly. All these studies suggested important roles of microRNAs in the developing ovaries.…”

Section: Discussionmentioning

confidence: 99%

microRNA 376a regulates follicle assembly by targeting Pcna in fetal and neonatal mouse ovaries

Zhang¹,

Jiang²,

Zhang³

et al. 2014

Reproduction

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In mammals, the primordial follicle pool, providing all oocytes available to a female throughout her reproductive life, is established perinatally. Dysregulation of primordial follicle assembly results in female reproductive diseases, such as premature ovarian insufficiency and infertility. Female mice lacking Dicer1 (Dicer), a gene required for biogenesis of microRNAs, show abnormal morphology of follicles and infertility. However, the contribution of individual microRNAs to primordial follicle assembly remains largely unknown. Here, we report that microRNA 376a (miR-376a) regulates primordial follicle assembly by modulating the expression of proliferating cell nuclear antigen (Pcna), a gene we previously reported to regulate primordial follicle assembly by regulating oocyte apoptosis in mouse ovaries. miR-376a was shown to be negatively correlated with Pcna mRNA expression in fetal and neonatal mouse ovaries and to directly bind to Pcna mRNA 3 0 untranslated region. Cultured 18.5 days postcoitum mouse ovaries transfected with miR-376a exhibited decreased Pcna expression both in protein and mRNA levels. Moreover, miR-376a overexpression significantly increased primordial follicles and reduced apoptosis of oocytes, which was very similar to those in ovaries co-transfected with miR-376a and siRNAs targeting Pcna. Taken together, our results demonstrate that miR-376a regulates primordial follicle assembly by modulating the expression of Pcna. To our knowledge, this is the first microRNA-target mRNA pair that has been reported to regulate mammalian primordial follicle assembly and further our understanding of the regulation of primordial follicle assembly.

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Section: Discussionmentioning

confidence: 99%

microRNA 376a regulates follicle assembly by targeting Pcna in fetal and neonatal mouse ovaries

Zhang¹,

Jiang²,

Zhang³

et al. 2014

Reproduction

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“…Bioinformatics and Gene Ontology analysis revealed that the target genes of these predominantly expressed miRNAs in the ovary are involved in cell cycle regulation; cellular growth, proliferation and apoptosis; endocrine system disorders; and ovarian functions [ 29 ]. In addition, a recent study demonstrated that miR-143 inhibited primordial follicle formation by reducing the expression of cyclin-dependent kinases (CDKs) 4 and 6 and cyclins B1, D2, and E2 in pregranulosa cells [ 30 ]. Furthermore, miR-181a inhibits mouse ovarian granulosa cell proliferation by targeting activin receptor IIA [ 31 ], and miR-26b promotes ovarian granulosa cell apoptosis by targeting the ataxia telangiectasia mutated (ATM) gene during follicular atresia [ 32 ].…”

Section: Mirna Profiles In the Ovarymentioning

confidence: 99%

“…Zhang et al revealed that miR-143 was expressed in pregranulosa cells using in situ hybridization. miR-143 inhibits the formation of primordial follicles by suppressing pregranulosa cell proliferation and downregulating the expression of genes related to the cell cycle, including cyclin D2, CDK4 and CDK6 [ 30 ]. During folliculogenesis, more than 99 % of ovarian follicles undergo atresia, and the roles of miRNAs in regulating follicle development and atresia were recently elucidated.…”

Section: Mirnas and Ovarian Functionmentioning

confidence: 99%

MicroRNAs in ovarian function and disorders

2015

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MicroRNAs (miRNAs) are endogenous, small, noncoding single-stranded RNA molecules approximately 22 nucleotides in length. miRNAs are involved in the post-transcriptional regulation of various important cellular physiological and pathological processes, including cell proliferation, differentiation, apoptosis, and hormone biosynthesis and secretion. Ovarian follicles are the key functional units of female reproduction, and the development of these follicles is a complex and precise process accompanied by oocyte maturation as well as surrounding granulosa cell proliferation and differentiation. Numerous miRNAs expressed in the ovary regulate ovarian follicle growth, atresia, ovulation and steroidogenesis and play an important role in ovarian disorders. This review considers recent advances in the identification of miRNAs involved in the regulation of ovarian function as well as the possible influence of miRNAs on ovarian-derived disorders, such as ovarian cancer, polycystic ovarian syndrome and premature ovarian failure. An improved understanding of the regulation of ovarian function by miRNAs may shed light on new strategies for ovarian biology and ovarian disorders.Electronic supplementary materialThe online version of this article (doi:10.1186/s13048-015-0162-2) contains supplementary material, which is available to authorized users.

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“…Regardless of mammalian species, miR-143 is among the most predominate miRNAs expressed in the ovary (70). Dysregulated miR-143, in mouse models, has been implicated in obesity-associated insulin resistance (71), in the formation of primordial follicles (72) and progesterone release (73).…”

Section: Discussionmentioning

confidence: 99%

Hyperandrogenism and Metabolic Syndrome Are Associated With Changes in Serum-Derived microRNAs in Women With Polycystic Ovary Syndrome

et al. 2019

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Polycystic ovary syndrome (PCOS) remains one of the most common endocrine disorder in premenopausal women with an unfavorable metabolic risk profile. Here, we investigate whether biochemical hyperandrogenism, represented by elevated serum free testosterone, resulted in an aberrant circulating microRNA (miRNAs) expression profile and whether miRNAs can identify those PCOS women with metabolic syndrome (MetS). Accordingly, we measured serum levels of miRNAs as well as biochemical markers related to MetS in a case-control study of 42 PCOS patients and 20 Controls. Patients were diagnosed based on the Rotterdam consensus criteria and stratified based on serum free testosterone levels (≥0.034 nmol/l) into either a normoandrogenic (n = 23) or hyperandrogenic (n = 19) PCOS group. Overall, hyperandrogenic PCOS women were more insulin resistant compared to normoandrogenic PCOS women and had a higher prevalence of MetS. A total of 750 different miRNAs were analyzed using TaqMan Low-Density Arrays. Altered levels of seven miRNAs (miR-485-3p, -1290, -21-3p, -139-3p, -361-5p, -572, and -143-3p) were observed in PCOS patients when compared with healthy Controls. Stratification of PCOS women revealed that 20 miRNAs were differentially expressed between the three groups. Elevated serum free testosterone levels, adjusted for age and BMI, were significantly associated with five miRNAs (miR-1290, -20a-5p, -139-3p, -433-3p, and -361-5p). Using binary logistic regression and receiver operating characteristic curves (ROC), a combination panel of three miRNAs (miR-361-5p, -1225-3p, and -34-3p) could correctly identify all of the MetS cases within the PCOS group. This study is the first to report comprehensive miRNA profiling in different subgroups of PCOS women with respect to MetS and suggests that circulating miRNAs might be useful as diagnostic biomarkers of MetS for a different subset of PCOS.

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miR-143 is critical for the formation of primordial follicles in mice

Cited by 45 publications

References 36 publications

microRNA 376a regulates follicle assembly by targeting Pcna in fetal and neonatal mouse ovaries

microRNA 376a regulates follicle assembly by targeting Pcna in fetal and neonatal mouse ovaries

MicroRNAs in ovarian function and disorders

Hyperandrogenism and Metabolic Syndrome Are Associated With Changes in Serum-Derived microRNAs in Women With Polycystic Ovary Syndrome

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