miR‐144/451 inhibits c‐Myc to promote erythroid differentiation

Xu, Lei; Wu, Fan; Yang, Lei; Wang, Fangfang; Zhang, Tong; Deng, Xintao; Zhang, Xiumei; Yuan, Xiaoling; Yan, Yan; Li, Yaoyao; Yang, Zhangping; Yu, Duonan

doi:10.1096/fj.202000941r

Cited by 20 publications

(18 citation statements)

References 48 publications

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“…MiRNAs target negative regulators of erythropoiesis (Table 1). [33][34][35][36][37][38][39][40][41][42][43][44][45] The deletion of miR-142 results in the biconcave shape of red blood cells, impaired structural elasticity, abnormal metabolism of reactive oxygen species (ROS), and shortened overall lifespan. 33,34 MiR-142 also maintains homeostatic erythropoiesis, hematopoietic progenitor proliferation, and normal enucleation by targeting Rac1 mRNA.…”

Section: Microrna Regulation In Erythropoiesismentioning

confidence: 99%

Post-transcriptional regulation of erythropoiesis

Zhang

et al. 2023

Blood Science

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Erythropoiesis is a complex, precise, and lifelong process that is essential for maintaining normal body functions. Its strict regulation is necessary to prevent a variety of blood diseases. Normal erythropoiesis is precisely regulated by an intricate network that involves transcription levels, signal transduction, and various epigenetic modifications. In recent years, research on post-transcriptional levels in erythropoiesis has expanded significantly. The dynamic regulation of splicing transitions is responsible for changes in protein isoform expression that add new functions beneficial for erythropoiesis. RNA-binding proteins adapt the translation of transcripts to the protein requirements of the cell, yielding mRNA with dynamic translation efficiency. Noncoding RNAs, such as microRNAs and lncRNAs, are indispensable for changing the translational efficiency and/or stability of targeted mRNAs to maintain the normal expression of genes related to erythropoiesis. N6-methyladenosine-dependent regulation of mRNA translation plays an important role in maintaining the expression programs of erythroid-related genes and promoting erythroid lineage determination. This review aims to describe our current understanding of the role of post-transcriptional regulation in erythropoiesis and erythroid-associated diseases, and to shed light on the physiological and pathological implications of the post-transcriptional regulation machinery in erythropoiesis. These may help to further enrich our understanding of the regulatory network of erythropoiesis and provide new strategies for the diagnosis and treatment of erythroid-related diseases.

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Section: Microrna Regulation In Erythropoiesismentioning

confidence: 99%

Post-transcriptional regulation of erythropoiesis

Zhang

et al. 2023

Blood Science

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“…The mKO and wild-type (WT) mice used in this study have been described in our previous publications (24,25). Male mice (C57BL/6, 10-12 weeks old, 23-25 g) were used in this study.…”

Section: Mice and Cell Linesmentioning

confidence: 99%

“…A miR-451 mimic and its negative control (miR-NC) were purchased from GenePharma Co., Ltd. (Shanghai, China). The MSCV-PIG and MSCV-miR-451 retroviral vectors have been described in our previous studies (24,25). All generic reagents were of analytical grade.…”

Section: Reagentsmentioning

confidence: 99%

“…A549 and HEK293T cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing 1% glutamine and 10% fetal bovine serum (FBS) in a humidified 37 ℃ incubator with 5% CO 2 . HEK293T cells were transfected with MSCV-PIG (Puromycin IRES GFP) and MSCV-miR-451 plasmids using Lipofectamine 2000 as previously described (25). A549 cells were infected with retroviral supernatant from 293T packaging cells.…”

Section: Infection and Lps Treatment Of A549 Cellsmentioning

confidence: 99%

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Deletion of the miR-144/451 cluster aggravates lethal sepsis-induced lung epithelial oxidative stress and apoptosis

Wu¹,

Yuan²,

Liu³

et al. 2022

Ann Transl Med

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Background: Sepsis is associated with a high mortality rate. A major cause of death in sepsis patients is respiratory failure, which is characterized by oxidative injury, epithelial apoptosis, and increased lung permeability. MicroRNAs (miRs) are important regulators of sepsis progression.Methods: This study aimed to explore the role of miR-144/451 in sepsis in mice. Experimental sepsis was induced in C57BL/6 mice by cecal ligation and puncture (CLP).Results: CLP significantly induced systemic inflammation, lung permeability, and lung epithelial apoptosis with downregulated messenger RNA (mRNA) levels of antioxidant enzymes. The miR-144/451 knockout mice had a lower 48-hour survival rate, higher plasma tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) levels, and greater pulmonary permeability compared with wild-type mice after CLP. CLP also markedly increased interstitial hemorrhage, collapsed more alveolar sacs, and increased the number of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive and Bcl-2-associated X (Bax)-positive cells in miR-144/451 knockout lung tissues, with elevated mRNA levels of Bax and reduced activities of catalase (Cat), glutathione peroxidase 1(Gpx1). MiR-451 negatively regulated 14-3-3ζ expression evidenced in miR-144/451 knockout lungs and the A549 cell line. In lipopolysaccharide (LPS)-induced A549 cells, miR-451 overexpression remarkably suppressed the production of reactive oxygen species, inhibited cell apoptosis, and enhanced levels of FoxO3 protein and related enzymes.Conclusions: Deletion of the miR-144/451 cluster aggravated sepsis-induced oxidative injury of lung epithelial cells.

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“…10 miR-144 is poorly expressed in liver, lung, and prostate cancer cells. 11–13 miR-144 appears closely related to human cancer, 10,13–15 and upregulating its expression may promote the proliferation of cervical cancer cells.…”

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confidence: 99%

Berberine Promotes A549 Cell Apoptosis and Autophagy via miR-144

Gao

Tan

Sha

2022

Natural Product Communications

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Objective: To explore the effects of berberine on A549 lung cancer cells and corresponding changes in miR-144 expression, and the apoptosis and autophagy pathways. Methods: Cell proliferation was detected by cell counting Kit-8. The expression of miR-144 by quantitative PCR, caspase-3, caspase-3 cleaved, Bcl-2, Bax, beclin-1, LC3I, and LC3II were assessed using Western blot. Results: A549 proliferation was reduced with increasing berberine concentration. Berberine appeared to suppress A549 proliferation through apoptosis and autophagy, and, additionally, enhanced miR-144 expression. Berberine promoted A549 cell apoptosis by inhibiting caspase-3 cleavage and Bcl-2 expression and promoting Bax expression. Berberine also promoted A549 autophagy by raising the expression of beclin-1, LC3I, and LC3II. Conclusions: Berberine promotes A549 apoptosis and autophagy via miR-144.

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miR‐144/451 inhibits c‐Myc to promote erythroid differentiation

Cited by 20 publications

References 48 publications

Post-transcriptional regulation of erythropoiesis

Post-transcriptional regulation of erythropoiesis

Deletion of the miR-144/451 cluster aggravates lethal sepsis-induced lung epithelial oxidative stress and apoptosis

Berberine Promotes A549 Cell Apoptosis and Autophagy via miR-144

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