MiR-144 regulates adipogenesis by mediating formation of C/EBPα-FOXO1 protein complex

Lin, Weimin; Wen, Xianyu; Li, Xuexin; Chen, Lei; Wei, Wei; Zhang, Lifan; Chen, Jie

doi:10.1016/j.bbrc.2022.04.093

Cited by 4 publications

(2 citation statements)

References 47 publications

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“…However, FoxO1- and C/EBPβ-binding may regulate pre-lipogenesis through the C/EBPβ-FoxO1-C/EBPβ feedback regulatory loop and the FoxO1-C/EBPβ protein complex, and FoxO1 and C/EBPβ co-knockout promote lipogenesis in adipocytes [ 37 ]. C/EBPα and FoxO1 ingest miR-144, forming a protein complex that binds to AdipoQ promoter, thus regulating AdipoQ transcription to inhibit fat formation [ 38 ]. It is well known that PPARγ can activate that expression of adipocyte differentiation regulators such as FABP4 , C/EBPs, and LPL to promote adipocyte differentiation for fat deposition [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

CircADAMTS16 Inhibits Differentiation and Promotes Proliferation of Bovine Adipocytes by Targeting miR-10167-3p

Xue

et al. 2023

Cells

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Circular RNAs (CircRNAs) are covalently closed-loop non-coding RNA (ncRNA) molecules present in eukaryotes. Numerous studies have demonstrated that circRNAs are important regulators of bovine fat deposition, but their precise mechanisms remain unclear. Previous transcriptome sequencing studies have indicated that circADAMTS16, a circRNA derived from the a disintegrin-like metalloproteinases with the thrombospondin motif 16 (ADAMTS16) gene, is high expressed in bovine adipose tissue. This gives a hint that the circRNA may be involved in the process of bovine lipid metabolism. In this study, the targeting relationship between circADAMTS16 and miR-10167-3p was verified using a dual-luciferase reporter assay. Then, the functions of circADAMTS16 and miR-10167-3p in bovine adipocytes were explored through gain-of-function and lose-of-function. The mRNA expression levels of genes were detected by real-time quantitative PCR (qPCR), and lipid droplet formation was phenotypically evaluated by Oil Red O staining. Cell proliferation and apoptosis were detected using CCK-8, EdU, and flow cytometry. We demonstrated that circADAMTS16 targeted binding to miR-10167-3p. The up-regulation of circADAMTS16 inhibited the differentiation of bovine preadipocytes, and the overexpression of miR-10167-3p promoted the differentiation of bovine preadipocytes. Meanwhile, CCK-8 and EdU results indicated that circADAMTS16 promoted adipocyte proliferation. Subsequently, flow cytometry analysis showed that circADAMTS16 promoted cell transition from G0/G1 phase to S phase, and inhibited cell apoptosis. However, up-regulation of miR-10167-3p inhibited cell proliferation and promoted apoptosis. Briefly, circADAMTS16 inhibited the differentiation and promotes the proliferation of bovine adipocytes by targeting miR-10167-3p during bovine fat deposition, which provides new insights into the mechanism of circRNAs regulation of beef quality.

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Section: Discussionmentioning

confidence: 99%

CircADAMTS16 Inhibits Differentiation and Promotes Proliferation of Bovine Adipocytes by Targeting miR-10167-3p

Xue

et al. 2023

Cells

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“…Alarmingly, the rates of obesity among children and adolescents have shown a substantial increase from 1975 to 2016 ( Lin et al, 2022a ). The root causes of obesity can be attributed to the excessive proliferation of adipocytes and the subsequent expansion of adipose tissue ( Lin et al, 2022b ). With the development of obesity, adipose tissue, particularly subcutaneous adipose tissue, exhibits a remarkable capacity to expand to adapt to energy storage demand requirements through a combination of adipocyte hypertrophy and hyperplasia ( Pellegrinelli et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Functional role of skeletal muscle-derived interleukin-6 and its effects on lipid metabolism

Lin¹,

Song²,

Shen³

et al. 2023

Front. Physiol.

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The detrimental impact of obesity on human health is increasingly evident with the rise in obesity-related diseases. Skeletal muscle, the crucial organ responsible for energy balance metabolism, plays a significant role as a secretory organ by releasing various myokines. Among these myokines, interleukin 6 (IL-6) is closely associated with skeletal muscle contraction. IL-6 triggers the process of lipolysis by mobilizing energy-storing adipose tissue, thereby providing energy for physical exercise. This phenomenon also elucidates the health benefits of regular exercise. However, skeletal muscle and adipose tissue maintain a constant interaction, both directly and indirectly. Direct interaction occurs through the accumulation of excess fat within skeletal muscle, known as ectopic fat deposition. Indirect interaction takes place when adipose tissue is mobilized to supply the energy for skeletal muscle during exercise. Consequently, maintaining a functional balance between skeletal muscle and adipose tissue becomes paramount in regulating energy metabolism and promoting overall health. IL-6, as a representative cytokine, participates in various inflammatory responses, including non-classical inflammatory responses such as adipogenesis. Skeletal muscle influences adipogenesis through paracrine mechanisms, primarily by secreting IL-6. In this research paper, we aim to review the role of skeletal muscle-derived IL-6 in lipid metabolism and other physiological activities, such as insulin resistance and glucose tolerance. By doing so, we provide valuable insights into the regulatory function of skeletal muscle-derived myokines in lipid metabolism.

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MiR-4769-3p suppresses adipogenesis in systemic sclerosis by negatively regulating the USP18/VDAC2 pathway

Tang,

Yu,

Tang

et al. 2024

iScience

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MiR-144 regulates adipogenesis by mediating formation of C/EBPα-FOXO1 protein complex

Cited by 4 publications

References 47 publications

CircADAMTS16 Inhibits Differentiation and Promotes Proliferation of Bovine Adipocytes by Targeting miR-10167-3p

CircADAMTS16 Inhibits Differentiation and Promotes Proliferation of Bovine Adipocytes by Targeting miR-10167-3p

Functional role of skeletal muscle-derived interleukin-6 and its effects on lipid metabolism

MiR-4769-3p suppresses adipogenesis in systemic sclerosis by negatively regulating the USP18/VDAC2 pathway

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