miR-145-5p inhibits the proliferation and migration of bladder cancer cells by targeting TAGLN2

Zhang, Haijian; Jiang, Meijuan; Liu, Qingjun; Han, Zhixing; Zhao, Yuqian; Ji, Shou‐Ping

doi:10.3892/ol.2018.9436

Cited by 42 publications

(86 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, it should be noted that the role of TAGLN2 regulating cell migration and invasion by affecting the cytoskeleton may not specifically function in the process of embryo implantation. Researches showed that TAGLN2 was highly expressed in kinds of malignant tumors and it could promote the invasiveness and metastasis (38)(39)(40). Besides, we observed high expression level of TAGLN2 in the decidualized mice endometrium, so we speculated that TAGLN2 may also be involved in endometrium remodeling through similar mechanisms to better adapt to the embryo implantation.…”

Section: Discussionmentioning

confidence: 72%

Transgelin 2 is required for embryo implantation by promoting actin polymerization

et al. 2019

View full text Add to dashboard Cite

Infertility has been a great challenge in reproductive medicine. At least 40% of human pregnancy losses are clinically unrecognized and occur because of embryo implantation failure. Identification of the proteins and biochemical factors involved in embryo implantation and that are essential for crosstalk between the embryo and uterus can further increase female fertility rates. The actin cytoskeleton and actin‐binding proteins (ABPs) are of great importance for cell morphology and rearrangement, which is crucial for trophoblast adhesion and invasion. However, the research on ABPs in embryo implantation is insufficient. In this report, we found that transgelin (TAGLN)2 is highly expressed in mouse blastocyst trophoblasts. Notably, inhibition of mouse blastocyst trophoblast TAGLN2 by lentivirus‐mediated RNA interference significantly impaired embryo adhesion and implantation ability. Further in vitro experiments demonstrated that TAGLN2 knockdown with small interfering RNA observably decreased the invasion and migration abilities of human trophoblast cells. Immunofluorescence colocalization and microscale thermophoresis analysis showed that TAGLN2 directly binds to actin. In addition, knockdown of TAGLN2 in trophoblast cells resulted in a remarkable reduction in F‐actin rather than G‐actin. Our findings reveal an unidentified role of TAGLN2 in regulation of trophoblast invasion and adhesion by promoting actin polymerization.—Liang, X., Jin, Y., Wang, H., Meng, X., Tan, Z., Huang, T., Fan, S. Transgelin 2 is required for embryo implantation by promoting actin polymerization. FASEB J. 33, 5667–5675 (2019). http://www.fasebj.org

show abstract

Section: Discussionmentioning

confidence: 72%

Transgelin 2 is required for embryo implantation by promoting actin polymerization

et al. 2019

View full text Add to dashboard Cite

show abstract

“…TAGLN2 is considered as a smooth muscle cytoskeletal protein ( 53 ). It has been proposed to be associated with growth and migration in bladder cancer ( 54 , 55 ), esophageal squamous cell carcinoma ( 56 ), and gliomas ( 57 ). Moreover, it's up-regulation is associated with tumorigenesis and tumor progression ( 54 , 58 ).…”

Section: Discussionmentioning

confidence: 99%

“…It has been proposed to be associated with growth and migration in bladder cancer ( 54 , 55 ), esophageal squamous cell carcinoma ( 56 ), and gliomas ( 57 ). Moreover, it's up-regulation is associated with tumorigenesis and tumor progression ( 54 , 58 ). Silence of TAGLN2 in gliomas cell lines significantly inhibited invasion and tumor growth ( 57 ).…”

Section: Discussionmentioning

confidence: 99%

Prognosis Analysis and Validation of m6A Signature and Tumor Immune Microenvironment in Glioma

Lin

Zhang

et al. 2020

Front. Oncol.

View full text Add to dashboard Cite

Glioma is one of the most typical intracranial tumors, comprising about 80% of all brain malignancies. Several key molecular signatures have emerged as prognostic biomarkers, which indicate room for improvement in the current approach to glioma classification. In order to construct a more veracious prediction model and identify the potential prognosis-biomarker, we explore the differential expressed m 6 A RNA methylation regulators in 665 gliomas from TCGA-GBM and TCGA-LGG. Consensus clustering was applied to the m6A RNA methylation regulators, and two glioma subgroups were identified with a poorer prognosis and a higher grade of WHO classification in cluster 1. The further chi-squared test indicated that the immune infiltration was significantly enriched in cluster 1, indicating a close relation between m 6 A regulators and immune infiltration. In order to explore the potential biomarkers, the weighted gene co-expression network analysis (WGCNA), along with Least absolute shrinkage and selection operator (LASSO), between high/low immune infiltration and m 6 A cluster 1/2 groups were utilized for the hub genes, and four genes ( TAGLN2, PDPN, TIMP1, EMP3) were identified as prognostic biomarkers. Besides, a prognostic model was constructed based on the four genes with a good prediction and applicability for the overall survival (OS) of glioma patients (the area under the curve of ROC achieved 0.80 (0.76–0.83) and 0.72 (0.68–0.76) in TCGA and Chinese Glioma Genome Atlas (CGGA), respectively). Moreover, we also found PDPN and TIMP1 were highly expressed in high-grade glioma from The Human Protein Atlas database and both of them were correlated with m6A and immune cell marker in glioma tissue samples. In conclusion, we construct a novel prognostic model which provides new insights into glioma prognosis. The PDPN and TIMP1 may serve as potential biomarkers for prognosis of glioma.

show abstract

“…In particular, miR-145-5p displays an essential malignancy-repressing impact on malignancy progression [26,27]. MiR-145-5p expression was repressed in several malignancies, such as non-smallcell lung cancer (NSCLC), ESCC, cervical cancer, and colorectal cancer, wherein it could repress malignancy generation [28][29][30]. Metadata proved that miR-145-5p is remarkably downregulated in NSCLC compared with that in normal tissues and could assist in a more accurate diagnosis in lung squamous cell carcinoma [31].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-145-5p regulates the proliferation of epithelial ovarian cancer cells via targeting SMAD4

Zhou¹,

Zhang²,

Li³

et al. 2020

J Ovarian Res

View full text Add to dashboard Cite

Background: Epithelial ovarian cancer (EOC) is one of the most prevalent malignancies affecting females worldwide; however, its etiology mechanism remains unclear. In various malignancies, miR-145-5p is a widely accepted and versatile miRNA. Therefore, our research focused on exploring the activity and etiology of miR-145-5p in the modulation of metastasis, migration, and proliferation of EOC cells. The direct reactions between the 3′UTRs of SMAD4 mRNA and miR-145-5p were verified using dual luciferase reporter test. SKOV-3 cells were subsequently transfected using miR-145-5p mimics. Cell migration, death, and proliferation were evaluated using MTT, flow cytometry, and Transwell test. In addition, SMAD4 transcription and translation were evaluated using qRT-PCR and Western blot. Results: We found that miR-145-5p expression was repressed prevalently in EOC tissues, apart from SMAD4 upregulation. Excessive miR-145-5p expression remarkably reinforced EOC cell death and repressed EOC cell proliferation. Furthermore, upregulated miR-145-5p expression noticeably repressed migration via MMP-2 and MMP-9 downregulation. Moreover, SMAD4 was downregulated via miR-145-5p transfection. The dual luciferase test revealed that miR-145-5p directly targeted SMAD4. Conclusions: Our research suggests that miR-145-5p serves as a malignancy repressor and exerts an essential impact on inhibiting malignancy generation and reinforcing EOC death via targeting SMAD4. MiR-145-5p application could serve as a promising strategy to treat EOC.

show abstract

miR-145-5p inhibits the proliferation and migration of bladder cancer cells by targeting TAGLN2

Cited by 42 publications

References 26 publications

Transgelin 2 is required for embryo implantation by promoting actin polymerization

Transgelin 2 is required for embryo implantation by promoting actin polymerization

Prognosis Analysis and Validation of m6A Signature and Tumor Immune Microenvironment in Glioma

MicroRNA-145-5p regulates the proliferation of epithelial ovarian cancer cells via targeting SMAD4

Contact Info

Product

Resources

About