MiR‐145, miR‐133a and miR‐133b inhibit proliferation, migration, invasion and cell cycle progression via targeting transcription factor Sp1 in gastric cancer

Abstract

Edited by Tamas Dalmay Keywords:Gastric cancer miR-145 miR-133a miR-133b Proliferation Migration Invasion Cell cycle progression Sp1 a b s t r a c t MicroRNAs have recently emerged as key regulators of gastric cancers. Here we found that miR-145, miR-133a and miR-133b were down-regulated in gastric cancer tissues and cell lines. Overexpression of miR-145, miR-133a and miR-133b induced G1 cell cycle arrest and inhibited cell proliferation, migration and invasion in vitro. MiR-145, miR-133a and miR-133b targeted the transcription factor SP1, knockdown of which reduced the expression of MMP-9 and Cyclin D1 that were involved in cell growth and invasion. Thus, our findings demonstrated for the first time that miR-145, miR133a and miR-133b suppressed the proliferation, migration, invasion and cell cycle progression of gastric cancer cells through decreasing expression of Sp1 and its downstream proteins.

MicroRNA-149 targets specificity protein 1 to suppress human tongue squamous cell carcinoma cell proliferation and motility

“…miR-29b and miR-375 function as tumor suppressors in TSCC through targeting SP1 (39,40). miR-145, miR-133a, miR-133b, miR-22 and miR-335 serve an important role in the biology of gastric cancer by regulating SP1 directly (41)(42)(43). miR-145 was also found to regulate SP1 in ovarian cancer cells sensitized to paclitaxel (44).…”

Propofol suppresses proliferation and invasion of pancreatic cancer cells by upregulating microRNA-133a expression

“…These results suggest that the antitumor effect of propofol on pancreatic cancer cells may be partly due to the upregulation of miR-133a. miR-133a downregulation has been confirmed in lung cancer (Moriya et al, 2012), breast cancer (Wu et al, 2012), rhabdomyosarcoma (Missiaglia et al, 2010), maxillary sinus squamous cell carcinoma (Nohata et al, 2011), tongue cancer (Wong et al, 2008), esophageal cancer (Kano et al, 2010), gastric cancer (Qiu et al, 2014), prostate cancer (Kojima et al, 2012), ovarian cancer (Guo et al, 2014), renal cell carcinoma , and pancreatic cancer (Qin et al, 2013). In these cancers, decreased miR-133a expression results in the induction of cell proliferation, the inhibition of apoptosis, or the promotion of cell invasion, indicating that miR-133a acts as a tumor-suppressor miRNA.…”

Downregulation of miR‐133b predict progression and poor prognosis in patients with urothelial carcinoma of bladder

“…In addition, Qiu et al. 27 stated that miR‐145, miR‐133a, and miR‐133b could inhibit proliferation, migration, invasion, and cell cycle progression of gastric cancer cells by regulating transcription factor Sp1. More recently, studies showed miR‐133b may affect the response of chemotherapy in certain cancer.…”