MiR-146a expression profiles in osteoarthritis in different tissue sources: a meta-analysis of observational studies

Liu, Jia-Neng; Liang, S.; Fu, Chang-ma

doi:10.1186/s13018-022-02989-7

Cited by 15 publications

(14 citation statements)

References 52 publications

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“…miR-146a is one of the most extensively studied miRNAs in relation to OA and inflammation. It has been found to have increased expression in cartilage of OA joints compared to controls in a number of previous studies in human patients (41,(66)(67)(68)(69), and has therefore been suggested as a biomarker of OA. Bioinformatics analysis has demonstrated that miR-146a targets 159 proteins (67), including inflammatory inducers in the nuclear factor kappa B pathway and IL-1β, and factors involved in apoptosis or autophagy (67), which might have been ongoing at the time our horses were euthanized (day 70 after OA-induction).…”

Section: Discussionmentioning

confidence: 99%

“…Three miRNAs were upregulated in cartilage from untreated OA joints compared to control joints (miR-146A; miR-150; miR-409-3p). Both miR-146a (36,41,(66)(67)(68)(69) and miR-150 (70,71) are known to be related to OA, whereas miR-409-3p (72, 73) has been described to be involved in regulation of inflammation, which is a prominent part of the OA pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

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Intraarticular treatment with integrin α10β1-selected mesenchymal stem cells affects microRNA expression in experimental post-traumatic osteoarthritis in horses

Andersen,

Walters,

Bundgaard

et al. 2024

Front. Vet. Sci.

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Osteoarthritis (OA) remains a major cause of lameness in horses, which leads to lost days of training and early retirement. Still, the underlying pathological processes are poorly understood. MicroRNAs (miRNAs) are small non-coding RNAs that serve as regulators of many biological processes including OA. Analysis of miRNA expression in diseased joint tissues such as cartilage and synovial membrane may help to elucidate OA pathology. Since integrin α10β1-selected mesenchymal stem cell (integrin α10-MSC) have shown mitigating effect on equine OA we here investigated the effect of integrin α10-MSCs on miRNA expression. Cartilage and synovial membrane was harvested from the middle carpal joint of horses with experimentally induced, untreated OA, horses with experimentally induced OA treated with allogeneic adipose-derived MSCs selected for the marker integrin α10-MSCs, and from healthy control joints. miRNA expression in cartilage and synovial membrane was established by quantifying 70 pre-determined miRNAs by qPCR. Differential expression of the miRNAs was evaluated by comparing untreated OA and control, untreated OA and MSC-treated OA, and joints with high and low pathology score. A total of 60 miRNAs were successfully quantified in the cartilage samples and 55 miRNAs were quantified in the synovial membrane samples. In cartilage, miR-146a, miR-150 and miR-409 had significantly higher expression in untreated OA joints than in control joints. Expression of miR-125a-3p, miR-150, miR-200c, and miR-499-5p was significantly reduced in cartilage from MSC-treated OA joints compared to the untreated OA joints. Expression of miR-139-5p, miR-150, miR-182-5p, miR-200a, miR-378, miR-409-3p, and miR-7177b in articular cartilage reflected pathology score. Several of these miRNAs are known from research in human patients with OA and from murine OA models. Our study shows that these miRNAs are also differentially expressed in experimental equine OA, and that expression depends on OA severity. Moreover, MSC treatment, which resulted in less severe OA, also affected miRNA expression in cartilage.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Intraarticular treatment with integrin α10β1-selected mesenchymal stem cells affects microRNA expression in experimental post-traumatic osteoarthritis in horses

Andersen,

Walters,

Bundgaard

et al. 2024

Front. Vet. Sci.

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“…Previous studies showed that miR-146a is a negative regulator of innate immune response [ 75 ], and is associated with inflammatory immune responses (e.g. systemic lupus erythematosus [ 76 ], osteoarthritis [ 77 ], rheumatoid arthritis [ 78 ]), tumors (e.g. colorectal cancer [ 79 ], breast cancer [ 80 ], gastric cancer [ 81 ], lung cancer [ 82 ]), angiogenesis [ 83 , 84 ], apoptosis [ 85 ], and autophagy [ 86 ].…”

Section: Discussionmentioning

confidence: 99%

Temporal transcriptomic changes in microRNAs involved in the host immune response and metabolism during Neospora caninum infection

et al. 2023

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Background Neospora caninum infection is a major cause of abortion in cattle, which results in serious economic losses to the cattle industry. However, there are no effective drugs or vaccines for the control of N. caninum infections. There is increasing evidence that microRNAs (miRNAs) are involved in many physiological and pathological processes, and dysregulated expression of host miRNAs and the biological implications of this have been reported for infections by various protozoan parasites. However, to our knowledge, there is presently no published information on host miRNA expression during N. caninum infection. Methods The expression profiles of miRNAs were investigated by RNA sequencing (RNA-seq) in caprine endometrial epithelial cells (EECs) infected with N. caninum at 24 h post infection (pi) and 48 hpi, and the functions of differentially expressed (DE) miRNAs were predicted by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. The transcriptome data were validated by using quantitative real-time polymerase chain reaction. One of the upregulated DEmiRNAs, namely chi-miR-146a, was selected to study the effect of DEmiRNAs on the propagation of N. caninum tachyzoites in caprine EECs. Results RNA-seq showed 18 (17 up- and one downregulated) and 79 (54 up- and 25 downregulated) DEmiRNAs at 24 hpi and 48 hpi, respectively. Quantitative real-time polymerase chain reaction analysis of 13 randomly selected DEmiRNAs (10 up- and three downregulated miRNAs) confirmed the validity of the RNA-seq data. A total of 7835 messenger RNAs were predicted to be potential targets for 66 DEmiRNAs, and GO and KEGG enrichment analysis of these predicted targets revealed that DEmiRNAs altered by N. caninum infection may be involved in host immune responses (e.g. Fc gamma R-mediated phagocytosis, Toll-like receptor signaling pathway, tumor necrosis factor signaling pathway, transforming growth factor-β signaling pathway, mitogen-activated protein kinase signaling pathway) and metabolic pathways (e.g. lysine degradation, insulin signaling pathway, AMP-activated protein kinase signaling pathway, Rap1 signaling pathway, calcium signaling pathway). Upregulated chi-miR-146a was found to promote N. caninum propagation in caprine EECs. Conclusions This is, to our knowledge, the first report on the expression profiles of host miRNAs during infection with N. caninum, and shows that chi-miR-146a may promote N. caninum propagation in host cells. The novel findings of the present study should help to elucidate the interactions between host cells and N. caninum. Graphical Abstract

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“…In particular, miR-34a has been known to be implicated in activating apoptosis signaling, limiting cell proliferation, and modulating redox balance in human OA chondrocytes and synovial fibroblasts [ 28 , 29 , 43 , 44 , 45 ]. In addition, miR-146a, traditionally identified as a regulator of inflammation, has been recently recognized as an activator of apoptosis and oxidative stress by its direct effect on SMAD4 and NRF2 transcriptional factors, in OA cells [ 28 , 29 , 46 ]. Similarly, miR-181a activates apoptosis and oxidative stress by targeting the anti-apoptotic marker BCL2 and modulating the oxidant/antioxidant system in different cell types [ 28 , 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

Synovial Fluid Regulates the Gene Expression of a Pattern of microRNA via the NF-κB Pathway: An In Vitro Study on Human Osteoarthritic Chondrocytes

Cheleschi

Tenti

Lorenzini

et al. 2022

IJMS

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Synovial fluid (SF) represents the primary source of nutrients of articular cartilage and is implicated in maintaining cartilage metabolism. We investigated the effects of SF, from patients with osteoarthritis (OA), rheumatoid arthritis (RA), and controls, on a pattern of microRNA (miRNA) in human OA chondrocytes. Cells were stimulated with 50% or 100% SF for 24 h and 48 h. Apoptosis and superoxide anion production were detected by cytometry; miRNA (34a, 146a, 155, 181a), cytokines, metalloproteinases (MMPs), type II collagen (Col2a1), antioxidant enzymes, B-cell lymphoma (BCL)2, and nuclear factor (NF)-κB by real-time PCR. The implication of the NF-κB pathway was assessed by the use of NF-κB inhibitor (BAY-11-7082). RA and OA SF up-regulated miR-34a, -146a, -155, -181a, interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, MMP-1, MMP-13, and ADAMTs-5 gene expression, while it down-regulated Col2a1. Pathological SF also induced apoptosis, reduced viability, and decreased BCL2 mRNA, whereas it increased superoxide anions, the expression of antioxidant enzymes, p65 and p50 NF-κB. Opposite and positive results were obtained with 100% control SF. Pre-incubation with BAY-11-7082 counteracted SF effects on miRNA. We highlight the role of the SF microenvironment in regulating some miRNA involved in inflammation and cartilage degradation during OA and RA, via the NF-κB pathway.

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MiR-146a expression profiles in osteoarthritis in different tissue sources: a meta-analysis of observational studies

Cited by 15 publications

References 52 publications

Intraarticular treatment with integrin α10β1-selected mesenchymal stem cells affects microRNA expression in experimental post-traumatic osteoarthritis in horses

Intraarticular treatment with integrin α10β1-selected mesenchymal stem cells affects microRNA expression in experimental post-traumatic osteoarthritis in horses

Temporal transcriptomic changes in microRNAs involved in the host immune response and metabolism during Neospora caninum infection

Synovial Fluid Regulates the Gene Expression of a Pattern of microRNA via the NF-κB Pathway: An In Vitro Study on Human Osteoarthritic Chondrocytes

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