miR‑148a‑3p suppresses epithelial ovarian cancer progression primarily by targeting c‑Met

Abstract

MicroRNAs (miRNAs) are a group of small non-coding RNAs that modulate post-transcriptional gene expression. It has been demonstrated that various miRNAs may be expressed at different levels in different types of tumors. The present study assessed the role of microRNA-148a-3p (miR-148a-3p) in epithelial ovarian cancer (EOC). The results demonstrated that miR-148a-3p was decreased in EOC tissues and that a lower miRa-148-3p concentration was associated with a higher overall survival rate. Transfection of miR-148a-3p suppressed the invasive and proliferative capacity of SKOV3 cells. The induced overexpression of miR-148a-3p significantly inhibited the relative luciferase activity of the pmirGLO-c-Met-3′untranslated region compared with an empty vector. In addition, c-Met silencing led to a decrease in the invasive and proliferative capacity of EOC cells. The inhibition of miR-148a-3p did not increase the invasiveness of SKOV3 cells, even when c-Met was silenced. To the best of our knowledge, the present study is the first to demonstrate that miR-148a-3p expression is decreased in EOC cancer tissues and cell lines. The present study therefore demonstrated that miR-148a-3p may serve as a tumor suppressor in EOC by targeting c-Met.

Expression level of EGFR and MET receptors regulates invasiveness of melanoma cells

“…Moreover, it was shown that down‐regulation of EGFR caused by MiR‐615 and MiR‐7, led to decreased migration, and invasion of human glioblastoma and ovarian cancer cells, respectively . Analogous effect was observed following MET silencing or its down‐regulation in many cancers like ovarian cancer, breast cancer, hepatocellular cancer or gastric cancer . Corso et al also indicated that MET silencing in already established metastases led to their almost complete regression.…”

“…36,37 Analogous effect was observed following MET silencing or its down-regulation in many cancers like ovarian cancer, breast cancer, hepatocellular cancer or gastric cancer. 38,39 Corso et al 40 Cell migration is the multi-step process, where formation of actin-rich protrusions is needed. 42 We have previously shown that EGF and HGF stimulate invadopodia formation, and extracellular matrix degradation, what correlates with higher invasive abilities of melanoma cells.…”

MicroRNA‐148a‐3p inhibits cancer progression and is a novel screening biomarker for gastric cancer

“…For instance, miR‐148a‐3p was reported to represses proliferation and EMT in bladder cancer by regulating ERBB3/AKT2/c‐myc and DNMT1 19 . miR‐148a‐3p could suppress ovarian cancer progression primarily by targeting c‐Met or suppress proliferation and invasion of esophageal cancer by targeting DNMT1 20,21 . Long noncoding RNA SNHG4/miR‐148a‐3p/c‐Met axis was reported to promote cervical cancer progression 22 .…”

HGF/c-MET: A Promising Therapeutic Target in the Digestive System Cancers

“…The HGF/c-MET receptor tyrosine kinase (RTK) pathway is quiescent in normal tissue, while it is active in various tumors [ 13 ]. Growing investigations have confirmed that inhibition of HGF/c-MET signaling is an effective therapeutic strategy in suppressing multiple human cancers, such as non-small cell lung cancer (NSCLC), HCC, gastric cancer, colorectal cancer ovarian cancer, bladder cancer, head and neck cancer, cervical cancer, and some other cancers [ 4 , 7 , 8 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. In preclinical and clinical trials, it has demonstrated that the inhibitors of c-MET have antitumor activity in treatment of NSCLC.…”