Mir-148a Improves Response to Chemotherapy in Sensitive and Resistant Oesophageal Adenocarcinoma and Squamous Cell Carcinoma Cells

Hummel, Richard; Watson, David I.; Smith, Cameron M.; Kist, Jakob W.; Michael, Michael; Haier, Joerg; Hussey, Damian J.

doi:10.1007/s11605-011-1418-9

Cited by 89 publications

(84 citation statements)

References 47 publications

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“…Increases in sample size, increase the likelihood of observing differences in expression. Recent studies have shown that miR-148a expression levels are significantly associated with differentiation grade (33) lymph node metastasis (34), and drug resistance in several cancers (35,36). Therefore, we next analyzed the relationship between miR-148a and clinicopathological characteristics in ovarian cancer, including chemotherapy prognosis and survival time.…”

Section: Mir-152 and Mir-148a Have No Effect On Invasion Of Skov3 Cellsmentioning

confidence: 99%

Altered expression of miR-152 and miR-148a in ovarian cancer is related to cell proliferation

Zhou

Zhao²,

Wang

et al. 2011

Oncol Rep

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Abstract. microRNAs (miRs) are endogenous small noncoding RNAs that are aberrantly expressed in various carcinomas. miR-152 and miR-148a have not been comprehensively investigated in ovarian cancer. Thus, the aim of this study was to identify the role of miR-152 and miR-148a in epithelial ovarian cancer. Total RNA was extracted from tissues of 78 patients with epithelial ovarian cancer, 17 normal ovarian epithelium tissues and two ovarian cancer cell lines. Using quantitative real-time PCR (qRT-PCR) followed by the 2 -ΔΔCT method for calculating the results, we found that the expression levels of miR-152 were significantly decreased in ovarian cancer tissues compared to normal ovarian epithelium tissues (p<0.05). However, although the expression of miR148a was also decreased in 65% of patients, no statistically significant difference in expression was found. A strong correlation was found between the expression of miR-152 and miR-148a (p<0.001, Pearson's correlation). The relationship between miR-152 or miR-148a expression levels in ovarian cancer and clinicopathological features, response to therapy and short-term survival was analyzed and the results showed that no correlation existed. In addition, we found that both miR-152 and miR-148a were down-regulated in ovarian cancer cell lines. After miR-152 or miR-148a mimics were transfected into ovarian cancer cell lines, the MTT cell proliferation assay showed that cell proliferation was significantly inhibited. Taken together, miR-152 and miR-148a may be involved in the carcinogenesis of ovarian cancer through deregulation of cell proliferation. They may be novel biomarkers for early detection or therapeutic targets of ovarian cancer. IntroductionOvarian cancer is one of the most common causes of death from gynecological malignancies. In addition, with an incidence of ~15,000 deaths annually, it is the fifth leading cause of cancer-related deaths among women in the United States (1). More than 90% of ovarian cancers are epithelial ovarian cancers (EOCs), which are derived from the ovarian surface epithelium (2). Due to a lack of effective biomarkers, ineffective tools for ovarian cancer screening, and non-specific symptoms in its early stages, more than two-thirds of patients with ovarian cancer are not diagnosed until the disease is in an advanced stage (3). Despite advances in early detection and the current standard treatment for advanced ovarian cancer, the 5-year survival rate is only 20-25% (4,5), which makes the development of alternative approaches urgent. Understanding the molecular alterations of ovarian cancer will help identify novel diagnostic markers or therapeutic targets, thereby improving the survival rates in this population of cancer patients.microRNAs (miRNAs or miRs) are a class of highly conserved, non-coding RNAs, approximately 19-25 nucleotides in length. They function as post-transcriptional regulators by binding to the 3'UTR regions of protein-encoding genes, which results in translational repression and gene silencing (6,7). Almost 30% ...

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Section: Mir-152 and Mir-148a Have No Effect On Invasion Of Skov3 Cellsmentioning

confidence: 99%

Altered expression of miR-152 and miR-148a in ovarian cancer is related to cell proliferation

Zhou

Zhao²,

Wang

et al. 2011

Oncol Rep

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“…Noteworthy, although there are no reports regarding the role of miR-148a in NSCLC, miR-148a may prove a novel Rh2 target in NSCLC. miR-148a was originally shown to be downregulated in human breast cancer; however, it is associated with improved response to chemotherapy in esophageal cancer cell lines, attenuates paclitaxel-resistance of hormonerefractory, drug-resistant prostate cancer PC3 cells, suppresses gastric cancer cell invasion and metastasis, and promotes apoptosis in colorectal cancer cells (20)(21)(22)(23)(24). Therefore, future studies should be directed towards elucidating the relationship between Rh2-mediated anticancer properties and miR-148a upregulation in NSCLC.…”

Section: Resultsmentioning

confidence: 99%

Ginsenoside Rh2 mediates changes in the microRNA expression profile of human non-small cell lung cancer A549 cells

An¹,

An²,

Kwon

et al. 2012

Oncology Reports

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Abstract. Non-small cell lung cancer (NSCLC) is the most common type of lung cancer insensitive to chemotherapy. Efforts are, therefore, directed toward understanding the molecular mechanisms of chemotherapy insensitivity and the development of new anticancer drugs. Ginsenoside Rh2, one of the components in ginseng saponin, has been shown to have anti-proliferative effect on human NSCLC cells and is being studied as a therapeutic drug for NSCLC. microRNAs (miRNAs) are small, non-coding RNA molecules that play a key role in cancer progression and prevention. However, the miRNA portrait of ginsenoside Rh2-treated NSCLC cells has not yet been studied. In this study, we identified a unique set of changes in the miRNA expression profile in response to Rh2 treatment in the human NSCLC cell line A549. Using miRNA microarray analysis, we identified 44 and 24 miRNAs displaying changes in expression greater than 2-fold in Rh2-treated A549 cells. In addition, using an miRNA target prediction program, we discovered that these miRNAs are predicted to have several target genes related to angiogenesis, apoptosis, chromatic modification, cell proliferation and differentiation. Thus, these results may assist in the better understanding of the anticancer mechanism of Rh2 in NSCLC.

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“…Previous studies have indicated that HLA-G expression level may be regulated by miR-148a, and that miR-148b and HLA-G were upregulated in primary ESCC tissues (13,16). Based on these reports, it was hypothesized that miR-148a may contribute to the proliferation of ESCC cells by regulating HLA-G expression.…”

Section: Effect Of Mir-148a On Hla-g Expressionmentioning

confidence: 99%

“…Hummel et al (15) reported that miR-148a was downregulated in patients with recurrent EC. They also demonstrated that in EC cell lines, upregulation of miR-148a improved sensitivity to chemotherapy (16). However, whether miR-148a is downregulated in primary ESCC remains unclear.…”

Section: Introductionmentioning

confidence: 99%

MiR‑148a modulates HLA‑G expression and influences tumor apoptosis in esophageal squamous cell carcinoma

2017

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Mir-148a Improves Response to Chemotherapy in Sensitive and Resistant Oesophageal Adenocarcinoma and Squamous Cell Carcinoma Cells

Cited by 89 publications

References 47 publications

Altered expression of miR-152 and miR-148a in ovarian cancer is related to cell proliferation

Altered expression of miR-152 and miR-148a in ovarian cancer is related to cell proliferation

Ginsenoside Rh2 mediates changes in the microRNA expression profile of human non-small cell lung cancer A549 cells

MiR‑148a modulates HLA‑G expression and influences tumor apoptosis in esophageal squamous cell carcinoma

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