MiR-148a promotes apoptosis by targeting Bcl-2 in colorectal cancer

Zhang, H; Li, Y; Huang, Qiong; Ren, Xiangxia; Hu, Hanqing; Sheng, Hongxu; Lai, Maode

doi:10.1038/cdd.2011.28

Cited by 184 publications

(157 citation statements)

References 36 publications

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“…36 Generally, miR-148a functions as a tumor suppressor in cancer cells. [33][34][35][36] However, in most studies, miR-148a was detected in specimens of tissues or cell lines. There is limited research involving exosomes containing miR-148a.…”

Section: Discussionmentioning

confidence: 99%

Characterization of exosomal RNAs derived from human gastric cancer cells by deep sequencing

Ren

Zhou

et al. 2017

Tumour Biol.

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Exosomes secreted from the cell to the extracellular environment play an important role in intercellular communication.Next-generation sequencing technology, which has achieved great development recently, allows us to detect more complete data and gain even deeper analyses of RNA transcriptomes. In our research, we extracted exosomes from different gastric cancer cell lines and immortalized normal gastric mucosal epithelial cell line and examined the amounts of exosomal proteins and RNAs. Our data showed that the secreted amount of cancer cell-derived exosomes, which contain proteins and RNAs, was much higher than that of normal cell-derived exosomes. Moreover, next-generation sequencing technology confirmed the presence of small non-coding RNAs in exosomes. Based on publicly available databases, we classified small non-coding RNAs. According to the microRNA profiles of exosomes, hsa-miR-21-5p and hsa-miR-30a-5p were two of the most abundant sequences among all libraries. The expression levels of the two microRNAs, miR-100 and miR-148a, in exosomes were validated through reverse transcription polymerase chain reaction. The reverse transcription polymerase chain reaction result, consistent with the trend of sequencing result, indicated a significant difference in exosomes between gastric cancer and gastric mucosal epithelial cell lines. We also predicted novel microRNA candidates but they need to be validated. This research provided an atlas of small noncoding RNA in exosomes and may make a little contribution to the understanding of exosomal RNA composition and finding parts of differential expression of RNAs in exosomes.

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Section: Discussionmentioning

confidence: 99%

Characterization of exosomal RNAs derived from human gastric cancer cells by deep sequencing

Ren

Zhou

et al. 2017

Tumour Biol.

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“…Previous studies have indicated that HLA-G expression level may be regulated by miR-148a, and that miR-148b and HLA-G were upregulated in primary ESCC tissues (13,16). Based on these reports, it was hypothesized that miR-148a may contribute to the proliferation of ESCC cells by regulating HLA-G expression.…”

Section: Effect Of Mir-148a On Hla-g Expressionmentioning

confidence: 99%

“…MiRs are also key regulators of gene transcription, and are frequently involved in the occurrence of cancers (9). In particular, miR-148a has been reported to be a tumor suppressor in multiple cancer types, including gastrointestinal (10), breast (11), ovarian (12), colorectal (13) and prostate cancer (14). Hummel et al (15) reported that miR-148a was downregulated in patients with recurrent EC.…”

Section: Introductionmentioning

confidence: 99%

MiR‑148a modulates HLA‑G expression and influences tumor apoptosis in esophageal squamous cell carcinoma

2017

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“…Previous research described miR-148a as a tumor suppressor in hepatocellular carcinoma, pancreatic cancer, gastric cancer and colorectal cancer. It was shown that microRNA-148a suppresses tumor cell invasion and metastasis by downregulating ROCK1 in gastric cancer [181], promotes apoptosis by targeting Bcl-2 in colorectal cancer [182], and suppresses the epithelial-mesenchymal transition and metastasis of hepatoma cells by targeting Met/Snail signaling [179]. However, we were not able to verify the above as targets of miR-148a in GBM.…”

Section: Mir-148a Inhibits Egfr Trafficking and Degradationmentioning

confidence: 69%

“…A thorough literature analysis revealed no publications on miR-148a in GBM. Previous published research had described miR-148a as a tumor suppressor in hepatocellular carcinoma, pancreatic cancer, gastric cancer and colorectal cancer [179][180][181][182]. However, a role for miR-148a in GBM and its characterization as oncogene have not been described before.…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of miR-148a as a New Oncogenic and Prognostic microRNA in Glioblastoma

Kim

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Great interest persists in useful prognostic and therapeutic targets in glioblastoma (GBM), the most common and most deadly human brain tumor. In this study, we report the identification and characterization of microRNA-148a (miR-148a) as a novel prognostic oncomiR in GBM. We show that miR-148a expression is elevated in human GBM specimens, cell lines, and stem cells (GSC) compared with normal human brain and astrocytes. High levels of miR-148a were a risk indicator for GBM patient survival. Functionally, miR-148a expression increased cell growth, survival, migration, and invasion in glioblastoma cells and GSCs and promoted GSC neurosphere formation. We identified two direct targets of miR-148a, the EGF receptor (EGFR) regulator MIG6 and the apoptosis regulator BIM. Rescue experiments showed that MIG6 and BIM were essential to mediate the oncogenic activity of miR-148a. By inhibiting MIG6 expression, miR148a reduced EGFR trafficking to Rab7-expressing compartments, which includes late endosomes and lysosomes. This process coincided with reduced degradation and elevated expression and activation of EGFR. Finally, inhibition of miR-148a strongly suppressed GSC and glioblastoma xenograft growth in vivo. Taken together, our findings provide a comprehensive analysis of the prognostic value and oncogenic function of miR-148a in glioblastoma, further defining it as a potential target for glioblastoma therapy.

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MiR-148a promotes apoptosis by targeting Bcl-2 in colorectal cancer

Cited by 184 publications

References 36 publications

Characterization of exosomal RNAs derived from human gastric cancer cells by deep sequencing

Characterization of exosomal RNAs derived from human gastric cancer cells by deep sequencing

MiR‑148a modulates HLA‑G expression and influences tumor apoptosis in esophageal squamous cell carcinoma

Identification and Characterization of miR-148a as a New Oncogenic and Prognostic microRNA in Glioblastoma

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