miR-153-3p inhibited osteogenic differentiation of human DPSCs through CBFβ signaling

Wei, Changbo; Chu, Manru; Zheng, Ke; He, Ping; Xiao, Jingwen

doi:10.1007/s11626-022-00665-y

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…New evidence has suggested that many of the differing methods and mechanisms used to induce differentiation in DPSCs may actually converge in common or shared regulatory mechanisms, such as the expression and modulation of non-coding microRNAs [22][23][24][25]. It has been established that these short, non-coding microRNAs may function to mediate and modulate the gene expression in many types of cells and tissues, and many microRNAs are now known to direct and regulate the osteogenic differentiation of DPSCs through both positive (induction) and negative (inhibitory) regulatory feedback mechanisms, such as miR-24, miR-31, miR-140, miR-143, miR-153, and miR-188 [26][27][28][29][30][31]. However, the mechanisms and processes of microRNA expression that regulate neuronal differentiation in DPSC are virtually unknown-although miR-27, miR-125, miR-128, miR-135, miR-140, miR-145, miR-218, and miR-410 have been identified as potential regulators of MSC neuronal differentiation [32][33][34][35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of MicroRNA (MiR-27, MiR-145) among Dental Pulp Stem Cells (DPSCs) Following Neurogenic Differentiation Stimuli

Bassett,

Triplett,

Lott

et al. 2023

Biomedicines

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This study sought to evaluate the expression of previously identified microRNAs known to regulate neuronal differentiation in mesenchymal stem cells (MSCs), including miR-27, miR-125, miR-128, miR-135, miR-140, miR-145, miR-218 and miR-410, among dental pulp stem cells (DPSCs) under conditions demonstrated to induce neuronal differentiation. Using an approved protocol, n = 12 DPSCs were identified from an existing biorepository and treated with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF), which were previously demonstrated to induce neural differentiation markers including Sox1, Pax6 and NFM among these DPSCs. This study revealed that some microRNAs involved in the neuronal differentiation of MSCs were also differentially expressed among the DPSCs, including miR-27 and miR-145. In addition, this study also revealed that administration of bFGF and EGF was sufficient to modulate miR-27 and miR-145 expression in all of the stimulus-responsive DPSCs but not among all of the non-responsive DPSCs—suggesting that further investigation of the downstream targets of these microRNAs may be needed to fully evaluate and understand these observations.

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Section: Introductionmentioning

confidence: 99%

Differential Expression of MicroRNA (MiR-27, MiR-145) among Dental Pulp Stem Cells (DPSCs) Following Neurogenic Differentiation Stimuli

Bassett,

Triplett,

Lott

et al. 2023

Biomedicines

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show abstract

“…Additional studies have demonstrated microRNAs may induce similar types of effects among mesenchymal stem cells to modulate osteogenic differentiation (Xie et al, 2020;Wang et al, 2019;Liu et al, 2020). However, only a few studies have evaluated these mechanisms and effects in DPSC (Liang et al, 2022;Fu et al, 2016;Wei et al, 2022). Pilot studies from this group have demonstrated differential expression of some microRNAs among DPSC isolates, including miR-217, miR-124, and miR-27 (Whiting et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Expression of Exosome-Derived MicroRNAs miR-21 and miR-135 are Differentially Regulated Among Dental Pulp Stem Cells

Hunsaker¹,

Richards²,

Fuller³

et al. 2023

Current Research in Medicine

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Dental Pulp Stem Cells (DPSCs) are a type of mesenchymal stem cell that has the potential to differentiate into various types of cells and tissue. Previous research has demonstrated that DPSCs can be easily accessed and isolated from both permanent and deciduous teeth, such as wisdom teeth or third molars, although there is less information available about the mechanisms and factors that regulate the growth and proliferative phenotypes and responses of DPSCs. Recent studies have revealed that a type of non-coding RNA known as microRNA can modulate these characteristics among many types of stem cells, although much is still unknown about how DPSC phenotypes may be regulated by microRNAs. Due to this lack of knowledge, the primary objective of this study was to evaluate microRNA expression and determine any correlations with DPSC phenotypes, such as proliferation or growth. Six DPSC isolates were retrieved from an existing repository and cultured using an existing approved protocol. Exosomes and extracellular vesicles were extracted from each DPSC isolate, which ranged in size from 50 to 250 nm. Exosome isolation was confirmed using Western blots for CD63 and Bradford protein assays. In addition, RNA was extracted, cDNA was synthesized, and qPCR was performed, which revealed that all DPSC isolates expressed miR-124, miR-133, and miR-224. However, differential expression of miR-21 among rapid and intermediate doubling time (rDT, iDT) isolates was observed, with expression of miR-135 found only among the intermediate and slow (iDT, sDT) DPSC isolates. This study provides some of the first evidence of associations between miRNA expression and specific DPSC growth phenotypes. Further studies will be needed to confirm these results and determine the mechanisms associated with the expression of miR-21 among rapidly growing DPSCs and miR-135 expression among more slowly growing DPSCs.

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Current concepts of microRNA-mediated regulatory mechanisms in human pulp tissue-derived stem cells: a snapshot in the regenerative dentistry

Soheilifar¹,

Nobari

Hakimi

et al. 2023

Cell Tissue Res

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miR-153-3p inhibited osteogenic differentiation of human DPSCs through CBFβ signaling

Cited by 4 publications

References 35 publications

Differential Expression of MicroRNA (MiR-27, MiR-145) among Dental Pulp Stem Cells (DPSCs) Following Neurogenic Differentiation Stimuli

Differential Expression of MicroRNA (MiR-27, MiR-145) among Dental Pulp Stem Cells (DPSCs) Following Neurogenic Differentiation Stimuli

Expression of Exosome-Derived MicroRNAs miR-21 and miR-135 are Differentially Regulated Among Dental Pulp Stem Cells

Current concepts of microRNA-mediated regulatory mechanisms in human pulp tissue-derived stem cells: a snapshot in the regenerative dentistry

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