MiR-155-5p and MiR-203a-3p Are Prognostic Factors in Soft Tissue Sarcoma

Abstract

Soft tissue sarcoma (STS) is a heterogeneous group of rare malignancies with a five-year survival rate of approximately 50%. Reliable molecular markers for risk stratification and subsequent therapy management are still needed. Therefore, we analyzed the prognostic potential of miR-155-5p and miR-203a-3p expression in a cohort of 79 STS patients. MiR-155-5p and miR-203a-3p expression was measured from tumor total RNA by qPCR and correlated with the demographic, clinicopathological, and prognostic data of the patients. Elevated miR-155-5p expression was significantly associated with increased tumor stage and hypoxia-associated mRNA/protein expression. High miR-155-5p expression and low miR-203a-3p expression, as well as a combination of high miR-155-5p and low miR-203a-3p expression, were significantly associated with poor disease-specific survival in STS patients in the Kaplan–Meier survival analyses (p = 0.027, p = 0.001 and p = 0.0003, respectively) and in the univariate Cox regression analyses (RR = 1.96; p = 0.031; RR = 2.59; p = 0.002 and RR = 4.76; p = 0.001, respectively), but not in the multivariate Cox regression analyses. In conclusion, the oncomiR miR-155-5p and the tumor suppressor-miR miR-203a-3p exhibit an association with STS patient prognosis and are suggested as candidates for risk assessment.

miR-203, fine-tunning neuroinflammation by juggling different components of NF‐κB signaling

“…Expression of miR-203 was enriched in keratinocytes and upregulated in inflammatory skin conditions, such as psoriatic lesions, where the expression of a series of cytokines was differentially regulated by miR-203 [ 14 ]. Aberrant expression of miR-203 was also reported in the tumorigenesis and cancer progressions of various tissue origins [ 27 – 30 ]. Contradictory results were presented in these studies with respect to the exact biological functions of miR-203 in regulating cell proliferation and cell inflammatory responses, at least partly explained by multiple downstream targets that had opposite impact on overlapping pathways.…”

Long non-coding RNA LINC01194 promotes the inflammatory response and apoptosis of lipopolysaccharide-treated MLE-12 cells through the miR-203a-3p/MIP-2 axis

“…The miR-203a gene is located on chromosome 14q32.33 (Griffiths-Jones et al 2006). It is aberrantly expressed in different diseases, such as osteosarcoma (Wu et al 2019), soft tissue sarcoma (Greither et al 2020), and pancreatic cancer (An and Zheng 2020), which suggests its expression to serve as a diagnostic marker. Interestingly, report shows that the miR-203a-3p is involved in the regulation of inflammation in diseases.…”

Bone and Soft Tissue Tumors: New Treatment Approaches

“…Simpson et al reported that a comparison of gene expression between canine osteosarcomas and non-tumor tissue showed 1281 significantly differentially expressed genes (839 lower and 442 elevated gene expression), a subset of which were validated by qRT-PCR and immunohistochemistry [ 2 ]. Furthermore, Greither et al investigated the influence of miR-155-5p and miR-203a-3p expression on prognosis in patients with soft tissue sarcomas [ 3 ]. This study showed that increased expression of miR-155-5p was significantly associated with increased tumor stage, and that high miR-155-5p expression and low miR-203a-3p expression were significantly associated with poor survival in patients with soft tissue sarcomas.…”