miR‐155 is involved in tumor progression of mycosis fungoides

Abstract

Biopsy specimens from 23 early stage and 19 tumorstage mycosis fungoides (MF) patients were evaluated for miR-155 expression by real-time qualitative PCR and compared with 15 biopsy specimens from patients with T-cell-rich inflammatory skin diseases. Significant upregulation of miR-155 was found in MF tumors compared with both early-stage MF lesions and controls. There was no difference in miR-155 expression between early-stage and inflammatory dermatoses. Using laser capture microdissection, it was found that miR-155 was significantly higher in the lymphoma cells in tumor stage compared with the intraepidermal lymphocytes in early stage. In contrast, there was no difference in miR-155 expression between the intraepidermal lymphocytes and the dermal lymphocytes in early-stage MF.These findings suggest that although miR-155 expression cannot serve to discriminate early-stage MF from inflammatory dermatoses; however, it is involved in the switch from the indolent early stage into the aggressive tumor stage of the disease.Abbreviations: CTCL, cutaneous T cell lymphoma; MF, mycosis fungoides; SS, sezary syndrome; FFPE, formalin fixed paraffin embedded; LCM, laser capture microdissection; DLE, discoid lupus erythematosus.

MicroRNA Expression Profiling and DNA Methylation Signature for Deregulated MicroRNA in Cutaneous T-Cell Lymphoma

“…The usefulness of some CTCL-related microRNAs as specific diagnostic or prognostic markers has been postulated and their potential role as promising targets has been proposed (Kopp et al, 2013a;Moyal et al, 2013;Schneider et al, 2012;Maj et al, 2012;Manfè et al, 2012;Manfè et al, 2013;McGirt et al, 2014;Ito et al, 2014).…”

New aspects of the clinicopathological features and treatment of mycosis fungoides and Sézary syndrome

“…Previous studies have shown that miR‐155, miR‐92a, miR‐93 and miR‐326 are found to be overexpressed in MF compared with inflammatory skin diseases. In addition, higher expression levels of miR‐155 correlate with the progression of MF . In folliculotropic MF, the expression levels of miR‐155, miR‐223, miR‐34a, miR‐181a and miR‐93‐5p are upregulated, while the expression levels of miR‐34a, miR‐181a, miR‐181b, miR‐93‐5p and miR‐326 are overexpressed in MF with the large cell transformation (Fig.…”

miRNA in mycosis fungoides and skin inflammation

“… in APMIS. Recently, miR‐155 expression was associated with severe disease . As IL‐15 expression in situ was also associated with severe disease, it is possibly that the correlation between miR‐155 expression and severe disease is a reflection of a specific cytokine environment with high levels of IL‐15, which in turn stimulates miR‐155 expression.…”