miR-15a inhibits cell proliferation and epithelial to mesenchymal transition in pancreatic ductal adenocarcinoma by down-regulating Bmi-1 expression

Guo, Shixiang; Xu, Xuejun; Tang, Yichen; Zhang, Chaobin; Li, Jian; Ouyang, Yueping; Ju, Jingfang; Bie, Ping; Wang, Huaizhi

doi:10.1016/j.canlet.2013.10.009

Cited by 77 publications

(60 citation statements)

References 31 publications

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“…Very interestingly, two of these transcripts were micro-RNAs miR128 and miR15a. miR128 is involved in the regulation of amyloid-β degradation in Alzheimer's disease and is involved in tumor suppression (53,54), and miR15a is also involved in tumor growth and its expression is correlated with plaque score in Alzheimer's disease (55,56). A survey of age-related microRNA expression changes in the neuronal stem cell niches of the short-lived annual fish Nothobranchius furzeri found that miR15a was abundant only in older animals (57).…”

Section: Discussionmentioning

confidence: 99%

Effects of aging on circadian patterns of gene expression in the human prefrontal cortex

Chen

Logan

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

238

219

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With aging, significant changes in circadian rhythms occur, including a shift in phase toward a “morning” chronotype and a loss of rhythmicity in circulating hormones. However, the effects of aging on molecular rhythms in the human brain have remained elusive. Here, we used a previously described time-of-death analysis to identify transcripts throughout the genome that have a significant circadian rhythm in expression in the human prefrontal cortex [Brodmann’s area 11 (BA11) and BA47]. Expression levels were determined by microarray analysis in 146 individuals. Rhythmicity in expression was found in ∼10% of detected transcripts (P < 0.05). Using a metaanalysis across the two brain areas, we identified a core set of 235 genes (q < 0.05) with significant circadian rhythms of expression. These 235 genes showed 92% concordance in the phase of expression between the two areas. In addition to the canonical core circadian genes, a number of other genes were found to exhibit rhythmic expression in the brain. Notably, we identified more than 1,000 genes (1,186 in BA11; 1,591 in BA47) that exhibited age-dependent rhythmicity or alterations in rhythmicity patterns with aging. Interestingly, a set of transcripts gained rhythmicity in older individuals, which may represent a compensatory mechanism due to a loss of canonical clock function. Thus, we confirm that rhythmic gene expression can be reliably measured in human brain and identified for the first time (to our knowledge) significant changes in molecular rhythms with aging that may contribute to altered cognition, sleep, and mood in later life.

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Section: Discussionmentioning

confidence: 99%

Effects of aging on circadian patterns of gene expression in the human prefrontal cortex

Chen

Logan

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

238

219

View full text Add to dashboard Cite

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“…And Bmi-1 immunoreactivity emerged as an independent prognostic factor [19]. In pancreatic cancer [4], patients with low Bmi-1 expression had a better survival outcome. We have meta-analyzed the association between Bmi-1 expression and the clinicopathological characteristics and prognosis of patients with various malignancies [24].…”

Section: Discussionmentioning

confidence: 99%

“…Bmi-1 is a member of the polycomb group which functions as a transcriptional repressor [3,4]. Bmi-1 gene is associated with tumor invasion and metastasis [5].…”

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confidence: 99%

Lower Bmi-1 Expression May Predict Longer Survival of Colon Cancer Patients

Zheng

Zhang³

et al. 2016

Cell Physiol Biochem

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Background: This study aimed to investigate the Bmi-1 expression and the clinical significance in colon cancer (CC). Patients and Methods: Bmi-1 expression in tumor tissue and the corresponding normal tissue was detected using immunohistological staining. The correlations between Bmi-1 expression and clinicopathological characteristics and the overall survival (OS) time were analyzed. Results: The median H-scores of Bmi-1 in CC tissues and the corresponding tissues were 80.0 (0-270) and 5.0 (0-90), with no statistically significant difference (Z=-13.7, P<0.001). Bmi-1 expression in CC tissues was not statistically correlated with any characteristics. The median OS times for CC patients with high or low Bmi-1 expression were 53.7 months and 44.9 months, respectively, with no statistically significant difference (P = 0.123). The survival rates of patients with low Bmi-1 expression were higher than those of patients with high Bmi-1 expression but the differences were not statistically significant. Conclusion: Bmi-1 expression in CC tissue is significantly higher than that in corresponding normal tissue. While there may be a trend towards improved survival, this is not statistically significant.

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“…Previous studies have established that EMT is associated with tumor invasiveness, metastasis and prognosis (18,19). Furthermore, a number of studies have identified functional associations between lncRNAs and key effectors of EMT during carcinogenesis and embryonic development, including LincRNA-ROR (20), MALAT-1 (21) and BANCR (22).…”

Section: Discussionmentioning

confidence: 99%

Upregulation of H19 promotes invasion and induces epithelial-to-mesenchymal transition in esophageal cancer

et al. 2015

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Abstract. Long non-coding RNAs (lncRNAs) have previously been reported to be involved in cancer invasion, proliferation and apoptosis. However, the association between the lncRNA, H19, and esophageal cancer (EC) has remained elusive. In the present study, reverse transcription quantitative-polymerase chain reaction revealed that the expression of H19 was significantly increased and associated with tumor depth and metastasis in 133 EC samples. Furthermore, MTT and Transwell assays revealed that overexpression of H19 in vitro promoted the proliferation and invasion of EC cell lines, whereas knockdown of H19 inhibited the proliferation and invasion of EC cell lines. In addition, it was identified that an upregulation of H19 induced epithelial-to-mesenchymal transition, while the opposite effect was observed following the downregulation of H19. In conclusion, H19 has a significant role in the development of EC and may serve as a potential prognostic marker and therapeutic target for EC. IntroductionEsophageal carcinoma (EC) is the eighth most aggressive and malignant type of cancer, with a high incidence that varies according to geographic location and ethnicity (1). Despite progress in the development of diagnostic and therapeutic options, the survival rates for EC patients remain poor. Therefore, the identification of novel genes involved in the tumorigenesis and development of EC is urgently required.Long non-coding RNAs (lncRNAs) are a class of RNAs that have been reported to be involved in the regulation, invasion, proliferation and apoptosis of multiple tumors (2,3). The association between H19 expression and the progression of various types of cancer has been demonstrated in previous studies. One study found that the overexpression of lncRNA H19 enhanced the carcinogenesis and metastasis of gastric cancer (4). MALAT-1, an abundant lncRNA present in many human cell types, has been suggested to regulate the alternative splicing of a subset of pre-messenger (m)RNAs by modulating serine/arginine splicing factor activity. This factor in turn regulates tissue or cell-type-specific alternative splicing in a phosphorylation-dependent manner (5). However, the role of H19 in EC is yet to be elucidated.The epithelial-to-mesenchymal transition (EMT) has an important role in the invasion of various types of cancer by transforming adherent and polarized epithelial cells into invasive and motile mesenchymal cells (6,7). A number of transcription factors involved in EMTs, including Twist and Snail, increase the expression level of mesenchymal markers, including fibronectin, collagen and Vimentin, and decrease the expression of epithelial markers, including E-cadherin. The breakdown of tight junctions results in the loss of epithelial markers and the acquisition of mesenchymal markers (8-10).In the present study, the expression levels of H19 in EC were investigated, in order to elucidate the role of H19 in EC. Materials and methods Clinical

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miR-15a inhibits cell proliferation and epithelial to mesenchymal transition in pancreatic ductal adenocarcinoma by down-regulating Bmi-1 expression

Cited by 77 publications

References 31 publications

Effects of aging on circadian patterns of gene expression in the human prefrontal cortex

Effects of aging on circadian patterns of gene expression in the human prefrontal cortex

Lower Bmi-1 Expression May Predict Longer Survival of Colon Cancer Patients

Upregulation of H19 promotes invasion and induces epithelial-to-mesenchymal transition in esophageal cancer

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