miR-181b functions as an oncomiR in colorectal cancer by targeting PDCD4

Liu, Yanqing; Ur-Rehman, Uzair; Guo, Yu; Liang, Hongwei; Cheng, Ruochuan; Yang, Fei; Hong, Yeting; Zhao, Chihao; Liu, Minghui; Yu, Mengchao; Zhou, Xinyan; Yin, Kai; Chen, Jiangning; Zhang, Junfeng; Zhang, Chenyu; Zhi, Feng; Chen, Xi

doi:10.1007/s13238-016-0313-2

Cited by 56 publications

(39 citation statements)

References 46 publications

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“…Increasing evidence has demonstrated that miRNAs have been implicated in the regulation of various physiological and pathological processes including cell growth, the cell cycle, cell division, apoptosis, invasion, metastasis and angiogenesis (12)(13)(14)(15). Over the past decade, aberrant expression of miRNAs has been implicated in a wide range of human types of cancer, such as miR-548b in glioma (16), miR-19b in gastric cancer (17), miR-181b in colorectal cancer (18) and miR-126 in hepatocellular carcinoma (19). An increasing number of studies have indicated that the dysregulation of miRNAs is closely associated with tumour occurrence and development (20,21).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-202 inhibits cell proliferation, migration and invasion of glioma by directly targeting metadherin

et al. 2017

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Glioma is the most common and aggressive type of primary malignant brain tumour. Increasing evidence has revealed that microRNAs play important roles in multiple biological processes related to glioma occurrence, development, diagnosis, treatment and prognosis. MicroRNA-202 (miR-202) has been studied in several types of human cancer, whereas the biological roles of miR-202 in glioma remain unknown. The present study, aimed to investigate the expression, clinical significance and biological roles of miR-202 in glioma, as well as its underlying molecular mechanism. We found that miR-202 was significantly downregulated in glioma tissues and cell lines. Low miR-202 expression was associated with Karnofsky performance status (KPS) score and World Health Organization (WHO) grade of glioma patients. Functional assays revealed that ectopic expression of miR-202 inhibited cell proliferation, migration and invasion of glioma. In addition, metadherin (MTDH) was identified as a direct target gene of miR-202 in glioma through bioinformatic analysis, luciferase reporter assay, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting. Furthermore, MTDH expression was upregulated and negatively correlated with miR-202 expression in clinical glioma tissues. MTDH knockdown had similar roles to miR-202 overexpression in glioma cells. Rescue experiments revealed that upregulation of MTDH reversed the suppression of glioma cell growth and metastasis by miR-202. Moreover, miR-202 impaired the PI3K/Akt and Wnt/β-catenin pathways. These results highlight the tumour-suppressive effect of miR-202 in glioma, thereby suggesting that miR-202 may be a potential therapeutic target for the treatment of patients with this malignancy.

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Section: Introductionmentioning

confidence: 99%

MicroRNA-202 inhibits cell proliferation, migration and invasion of glioma by directly targeting metadherin

et al. 2017

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“…To date, there are three main approaches to potential miRNA-targeting therapies: expression vectors (miRNA sponges), small-molecule inhibitors and synthetic oligonucleotides [32]. For example, aberrantly activated miRNAs can be silenced using antagomirs, and re-expression of miRNAs that are lost in cancers can be achieved by the induction of miRNA mimics [18, 33, 34]. It has been found that aberrant expression of the miR-520 family occurs in malignant tumours, and transcripts in this miRNA family have been identified as key regulators of oncogenes [35].…”

Section: Discussionmentioning

confidence: 99%

A Novel Role for MiR-520a-3p in Regulating EGFR Expression in Colorectal Cancer

Zhang

Liu

et al. 2017

Cell Physiol Biochem

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Background/Aims: MicroRNAs (miRNAs) have been consistently demonstrated to be involved in colorectal cancer as either tumour oncogenes or tumour suppressors. However, the detailed role of miR-520a-3p in colorectal cancer remains poorly understood. Methods: Quantitative RT-PCR and western blotting assays were used to measure miR-520a-3p and EGFR expression levels in colorectal cancer tissues, respectively. Luciferase reporter assay was employed to validate the direct targeting of EGFR by miR-520a-3p. Cell migration, apoptosis and cell cycle assays were performed to analyse the biological functions of miR-520a-3p and EGFR in colorectal cancer cells. In vivo experiment was performed to analyse the effects of miR-520a-3p and EGFR on the growth of colorectal cancer xenografts in mice. Results: In this study, we found that miR-520a-3p was most likely to target the EGFR 3’-UTR, which was experimentally validated. In addition, we investigated the biological effects of EGFR inhibition by miR-520a-3p both in vitro and In vivo and found that miR-520a-3p could suppress cell migration, promote apoptosis, lead to colorectal cancer cell cycle arrest at the G0/G1 phase, and decelerate tumour growth in xenograft mice, potentially by targeting EGFR. Conclusions: This study highlights a tumour suppressor role for miR-520a-3p in colorectal cancer via the regulation of EGFR expression. Thus, miR-520a-3p may be a novel molecular therapeutic target for colorectal cancer.

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“…Among the myriad CRC-related molecular factors, oncogene activation (e.g., KRAS and IGF1R) and tumor suppressor gene silencing (e.g., APC and PDCD4) play vital roles during CRC tumorigenesis [6–9]. T-cell intracellular antigen 1 (TIA1) is an RNA binding protein and is linked to multiple biologic processes associated with RNA metabolism, both in the nucleus and in the cytoplasm [10].…”

Section: Introductionmentioning

confidence: 99%

miR-19a promotes colorectal cancer proliferation and migration by targeting TIA1

et al. 2017

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BackgroundColorectal cancer (CRC) is a major worldwide health problem due to its high prevalence and mortality rate. T-cell intracellular antigen 1 (TIA1) is an important tumor suppressor involved in many aspects of carcinogenesis and cancer development. How TIA1 expression is regulated during CRC development remains to be carefully elucidated.MethodsIn CRC tissue sample pairs, TIA1 protein and mRNA levels were monitored by Western blot and qRT-PCR, respectively. Combining meta-analysis and miRNA target prediction software, we could predict microRNAs that targeted TIA1. Next, three CRC cell lines (SW480, Caco2 and HT29) were used to demonstrate the direct targeting of TIA1 by miR-19a. In addition, we investigated the biological effects of TIA1 inhibition by miR-19a both in vitro by CCK-8, EdU, Transwell, Ki67 immunofluorescence and Colony formation assays and in vivo by a xenograft mice model.ResultsIn colorectal cancer (CRC), we found that TIA1 protein, but not its mRNA, was downregulated. We predicted that TIA1 was a target of miR-19a and validated that miR-19a binded directly to the 3’-UTR of TIA1 mRNA. miR-19a could promote cell proliferation and migration in CRC cells and accelerated tumor growth in xenograft mice by targeting TIA1.ConclusionsThis study highlights an oncomiR role for miR-19a in regulating TIA1 in CRC and suggests that miR-19a may be a novel molecular therapeutic target for CRC.Electronic supplementary materialThe online version of this article (doi:10.1186/s12943-017-0625-8) contains supplementary material, which is available to authorized users.

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miR-181b functions as an oncomiR in colorectal cancer by targeting PDCD4

Cited by 56 publications

References 46 publications

MicroRNA-202 inhibits cell proliferation, migration and invasion of glioma by directly targeting metadherin

MicroRNA-202 inhibits cell proliferation, migration and invasion of glioma by directly targeting metadherin

A Novel Role for MiR-520a-3p in Regulating EGFR Expression in Colorectal Cancer

miR-19a promotes colorectal cancer proliferation and migration by targeting TIA1

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