MiR-181d-5p regulates implant surface roughness-induced osteogenic differentiation of bone marrow stem cells

Liu, Yanping; Wang, Yixiang; Cheng, Xian; Zheng, Yinggan; Lyu, Mingyue; Di, Ping; Lin, Ye

doi:10.1016/j.msec.2020.111801

Cited by 25 publications

(17 citation statements)

References 54 publications

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“…, wettability, roughness and topography) of biomaterials play important roles in regulating cell behaviors, such as cell attachment and proliferation. 31 In this work, PI showed a flat surface, while PM20 and PM40 presented a rough surface with many MD nanosheets, leading to a submicro-structured surface. The surface roughness of PI was the lowest, followed by PM20, and that for PM40 was the highest, which was ascribed to more MD on the surface.…”

Section: Discussionmentioning

confidence: 55%

Molybdenum disulfide nanosheet/polyimide composites with improved tribological performances, surface properties, antibacterial effects and osteogenesis for facilitating osseointegration

et al. 2022

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Section: Discussionmentioning

confidence: 55%

Molybdenum disulfide nanosheet/polyimide composites with improved tribological performances, surface properties, antibacterial effects and osteogenesis for facilitating osseointegration

et al. 2022

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“…The rough surfaces caused by PDA and Cu deposition were hypothesized to be beneficial for subsequent cellular behaviors as increased surface roughness was reported to enhance entrapment of fibrin proteins, thus leading to improved adhesion of osteogenic cells and scaffold stability. There were numerous other scientific reports stating on the positive correlation between surface roughness, cellular attachment, proliferation and osteogenic capabilities [ 36 , 37 , 38 ].…”

Section: Resultsmentioning

confidence: 99%

Combined Effects of Polydopamine-Assisted Copper Immobilization on 3D-Printed Porous Ti6Al4V Scaffold for Angiogenic and Osteogenic Bone Regeneration

Lin

Lee

et al. 2022

Cells

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Numerous studies have demonstrated that biological compounds and trace elements such as dopamine (DA) and copper ions (Cu) could be modified onto the surfaces of scaffolds using a one-step immersion process which is simple, inexpensive and, most importantly, non-cytotoxic. The development and emergence of 3D printing technologies such as selective laser melting (SLM) have also made it possible for us to fabricate bone scaffolds with precise structural designs using metallic compounds. In this study, we fabricated porous titanium scaffolds (Ti) using SLM and modified the surface of Ti with polydopamine (PDA) and Cu. There are currently no other reported studies with such a combination for osteogenic and angiogenic-related applications. Results showed that such modifications did not affect general appearances and microstructural characteristics of the porous Ti scaffolds. This one-step immersion modification allowed us to modify the surfaces of Ti with different concentrations of Cu ions, thus allowing us to fabricate individualized scaffolds for different clinical scenarios. The modification improved the hydrophilicity and surface roughness of the scaffolds, which in turn led to promote cell behaviors of Wharton’s jelly mesenchymal stem cells. Ti itself has high mechanical strength, therefore making it suitable for surgical handling and clinical applications. Furthermore, the scaffolds were able to release ions in a sustained manner which led to an upregulation of osteogenic-related proteins (bone alkaline phosphatase, bone sialoprotein and osteocalcin) and angiogenic-related proteins (vascular endothelial growth factor and angiopoietin-1). By combining additive manufacturing, Ti6Al4V scaffolds, surface modification and Cu ions, the novel hybrid 3D-printed porous scaffold could be fabricated with ease and specifically benefited future bone regeneration in the clinic.

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“…The SEM images of the scaffolds on day 0 showed that all the scaffolds had rough irregular surfaces, with CA20 having more irregular contours than the rest of the groups. Liu et al reported that material surface roughness has an important role to play in modulating cellular activities [ 38 ]. In their study, the bone marrow stem cells were noted to have higher levels of osteogenic capabilities when cultured on micro-rough surfaces than cells cultured on smooth surfaces.…”

Section: Resultsmentioning

confidence: 99%

Additive Manufacturing of Caffeic Acid-Inspired Mineral Trioxide Aggregate/Poly-ε-Caprolactone Scaffold for Regulating Vascular Induction and Osteogenic Regeneration of Dental Pulp Stem Cells

Tien

Lee

et al. 2021

Cells

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Mineral trioxide aggregate (MTA) is a common biomaterial used in endodontics regeneration due to its antibacterial properties, good biocompatibility and high bioactivity. Surface modification technology allows us to endow biomaterials with the necessary biological targets for activation of specific downstream functions such as promoting angiogenesis and osteogenesis. In this study, we used caffeic acid (CA)-coated MTA/polycaprolactone (PCL) composites and fabricated 3D scaffolds to evaluate the influence on the physicochemical and biological aspects of CA-coated MTA scaffolds. As seen from the results, modification of CA does not change the original structural characteristics of MTA, thus allowing us to retain the properties of MTA. CA-coated MTA scaffolds were shown to have 25% to 55% higher results than bare scaffold. In addition, CA-coated MTA scaffolds were able to significantly adsorb more vascular endothelial growth factors (p < 0.05) secreted from human dental pulp stem cells (hDPSCs). More importantly, CA-coated MTA scaffolds not only promoted the adhesion and proliferation behaviors of hDPSCs, but also enhanced angiogenesis and osteogenesis. Finally, CA-coated MTA scaffolds led to enhanced subsequent in vivo bone regeneration of the femur of rabbits, which was confirmed using micro-computed tomography and histological staining. Taken together, CA can be used as a potently functional bioactive coating for various scaffolds in bone tissue engineering and other biomedical applications in the future.

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MiR-181d-5p regulates implant surface roughness-induced osteogenic differentiation of bone marrow stem cells

Cited by 25 publications

References 54 publications

Molybdenum disulfide nanosheet/polyimide composites with improved tribological performances, surface properties, antibacterial effects and osteogenesis for facilitating osseointegration

Molybdenum disulfide nanosheet/polyimide composites with improved tribological performances, surface properties, antibacterial effects and osteogenesis for facilitating osseointegration

Combined Effects of Polydopamine-Assisted Copper Immobilization on 3D-Printed Porous Ti6Al4V Scaffold for Angiogenic and Osteogenic Bone Regeneration

Additive Manufacturing of Caffeic Acid-Inspired Mineral Trioxide Aggregate/Poly-ε-Caprolactone Scaffold for Regulating Vascular Induction and Osteogenic Regeneration of Dental Pulp Stem Cells

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