miR-190a-5p participates in the regulation of hypoxia-induced pulmonary hypertension by targeting KLF15 and can serve as a biomarker of diagnosis and prognosis in chronic obstructive pulmonary disease complicated with pulmonary hypertension

Jiang, Jing; Xia, Yanfei; Liang, Yi; Yang, Meiling; Zeng, Wen; Zeng, Xiaocong

doi:10.2147/copd.s182504

Cited by 25 publications

(12 citation statements)

References 47 publications

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“…In this study, expression of rno-miR-190a-5p and rno-miR-374-5p in iliopsoas muscle was increased with decreasing body temperature. Previous reports suggest that the expression of both miRNAs fluctuates dynamically in response to hypoxia 24 , 25 . Hypothermic conditions induce tissue asphyxiation because blood is cooled by the inhalation of low-temperature air, and oxygen and hemoglobin do not dissociate in a cold environment.…”

Section: Discussionmentioning

confidence: 98%

Body temperature-dependent microRNA expression analysis in rats: rno-miR-374-5p regulates apoptosis in skeletal muscle cells via Mex3B under hypothermia

Umehara

Kagawa

Tomida

et al. 2020

Sci Rep

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Forensic diagnosis of fatal hypothermia is considered difficult because there are no specific findings. Accordingly, exploration of novel fatal hypothermia-specific findings is important. To elucidate the molecular mechanism of homeostasis in hypothermia and identify novel molecular markers to inform the diagnosis of fatal hypothermia, we focused on microRNA expression in skeletal muscle, which plays a role in cold-induced thermogenesis in mammals. We generated rat models of mild, moderate, and severe hypothermia, and performed body temperature-dependent microRNA expression analysis of the iliopsoas muscle using microarray and quantitative real-time PCR (qRT-PCR). The results show that rno-miR-374-5p expression was significantly induced only by severe hypothermia. Luciferase reporter assay and qRT-PCR results indicated that Mex3B expression was regulated by rno-miR-374-5p and decreased with decreasing body temperature. Gene ontology analysis indicated the involvement of Mex3B in positive regulation of GTPase activity. siRNA analysis showed that Mex3B directly or indirectly regulated Kras expression in vitro, and significantly changed the expression of apoptosis-related genes and proteins. Collectively, these results indicate that rno-miR-374-5p was activated by a decrease in body temperature, whereby it contributed to cell survival by suppressing Mex3B and activating or inactivating Kras. Thus, rno-miR-374-5p is a potential supporting marker for the diagnosis of fatal hypothermia.

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Section: Discussionmentioning

confidence: 98%

Body temperature-dependent microRNA expression analysis in rats: rno-miR-374-5p regulates apoptosis in skeletal muscle cells via Mex3B under hypothermia

Umehara

Kagawa

Tomida

et al. 2020

Sci Rep

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“…A Number of biomacromolecules have been dig out that has the possibility to be the diagnostic molecular such as miR-125b, miR-146a/b, mir-190a-5p and miR-210 [20][21][22][23]. However, none of them has been applied to clinical use.…”

Section: Discussionmentioning

confidence: 99%

CircRNA0001859, a new diagnostic and prognostic biomarkers for COPD and AECOPD

et al. 2020

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Background Dysregulation of circRNAs has been reported to be functionally associated with chronic obstructive pulmonary disease (COPD). The present investigation elucidated the potential role of CircRNA0001859 in regulating chronic obstructive pulmonary disease acute (COPD) and Acute Exacerbation of COPD (AECOPD). Methods Mice model of COPD was established to screen and verify the dysregulated expression of CircRNA0001859. Fluorescence in situ hybridization (FISH) and quantitative real-time PCR (qRT-PCR) were carried out to detect the expression of CircRNA0001859. 38 stable COPD patients, 24 AECOPD patients, 57 COPD with lung cancer patients and 28 healthy person with age and sex matched to total patients were used for the present investigation. Results circRNA0001859 was downregulated in the lung tissue of mice after the three kinds of treatments (Cigarette smoke (CS)/NK alone or CS + NNK) for inducing COPD. FISH assay verified the downregulation of circRNA0001859 both in the mice lung and human bronchial epithelial cell of COPD model. Furthermore,, the level of circRNA0001859 was also downregulated in the peripheral blood of COPD and lung cancer patients. CircRNA0001859 might act as a diagnostic and prognostic biomarker for the treatment of in COPD and AECOPD with Are under the receiver operating characteristic curve (ROC curve) (AUC) of 0.7433 and 0.8717, respectively. Conclusion We explored a novel circRNA0001859, which might act as a potential therapeutic biomarker for the treatment of COPD and AECOPD.

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“…miRNA, which can regulate gene expression by incompletely or completely directly binding to mRNA 3′-untranslated region (UTR), also interacts with promoters, coding DNA sequence (CDS), and 5′-UTR to participate in gene regulation. It plays an important role in regulating gene expression, organism development, and apoptosis [73][74][75][76][77].…”

Section: Mirna and Pulmonary Vascular Endothelial Cell Apoptosismentioning

confidence: 99%

The role of cigarette smoke-induced pulmonary vascular endothelial cell apoptosis in COPD

2021

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Chronic obstructive pulmonary disease (COPD) is one of the most common chronic respiratory diseases with high morbidity and mortality. It has become the fifth most burdened and the third most deadly disease in the global economy and increases year by year. The prevention and treatment of COPD are urgent. Smoking is the main and most common risk factor for COPD. Cigarette smoke (CS) contains a large number of toxic substances, can cause a series of changes in the trachea, lung tissue, pulmonary blood vessels, and promotes the occurrence and development of COPD. In recent years, the development of epigenetics and molecular biology have provided new guidance for revealing the pathogenesis, diagnosis, and treatment of diseases. The latest research indicates that pulmonary vascular endothelial cell apoptosis initiates and participates in the pathogenesis of COPD. In this review, we summarize the current research on the epigenetic mechanisms and molecular biology of CS-induced pulmonary vascular endothelial cell apoptosis in COPD, providing a new research direction for pathogenesis of COPD and a new target for the diagnosis, treatment, and prevention of COPD.

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miR-190a-5p participates in the regulation of hypoxia-induced pulmonary hypertension by targeting KLF15 and can serve as a biomarker of diagnosis and prognosis in chronic obstructive pulmonary disease complicated with pulmonary hypertension

Cited by 25 publications

References 47 publications

Body temperature-dependent microRNA expression analysis in rats: rno-miR-374-5p regulates apoptosis in skeletal muscle cells via Mex3B under hypothermia

Body temperature-dependent microRNA expression analysis in rats: rno-miR-374-5p regulates apoptosis in skeletal muscle cells via Mex3B under hypothermia

CircRNA0001859, a new diagnostic and prognostic biomarkers for COPD and AECOPD

The role of cigarette smoke-induced pulmonary vascular endothelial cell apoptosis in COPD

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