miR-193b-3p Promotes Proliferation of Goat Skeletal Muscle Satellite Cells through Activating IGF2BP1

Abstract

As a well-known cancer-related miRNA, miR-193b-3p is enriched in skeletal muscle and dysregulated in muscle disease. However, the mechanism underpinning this has not been addressed so far. Here, we probed the impact of miR-193b-3p on myogenesis by mainly using goat tissues and skeletal muscle satellite cells (MuSCs), compared with mouse C2C12 myoblasts. miR-193b-3p is highly expressed in goat skeletal muscles, and ectopic miR-193b-3p promotes MuSCs proliferation and differentiation. Moreover, insulin-like growth factor-2 mRNA-binding protein 1 (IGF2BP1) is the most activated insulin signaling gene when there is overexpression of miR-193b-3p; the miRNA recognition element (MRE) within the IGF1BP1 3′ untranslated region (UTR) is indispensable for its activation. Consistently, expression patterns and functions of IGF2BP1 were similar to those of miR-193b-3p in tissues and MuSCs. In comparison, ectopic miR-193b-3p failed to induce PAX7 expression and myoblast proliferation when there was IGF2BP1 knockdown. Furthermore, miR-193b-3p destabilized IGF2BP1 mRNA, but unexpectedly promoted levels of IGF2BP1 heteronuclear RNA (hnRNA), dramatically. Moreover, miR-193b-3p could induce its neighboring genes. However, miR-193b-3p inversely regulated IGF2BP1 and myoblast proliferation in the mouse C2C12 myoblast. These data unveil that goat miR-193b-3p promotes myoblast proliferation via activating IGF2BP1 by binding to its 3′ UTR. Our novel findings highlight the positive regulation between miRNA and its target genes in muscle development, which further extends the repertoire of miRNA functions.

Divergent Regulatory Roles of Transcriptional Variants of the Chicken LDB3 Gene in Muscle Shaping

“…Insulin-like growth factor-2 mRNA-binding protein 1 ( IGF2BP1 ) is an IGF2BP that plays a major role in RNA transport, translation, and stability and regulates cell proliferation and differentiation as an N6-methyladenosine (m6A) reader. − Knockout of IGF2BP1 in mice led to dwarfism, intestinal mucosa, and actin mRNA dysregulation and disorganization in the developing neocortex. − Evidence from genome-wide association studies and pangenomes revealed that mutations in the promoter region of IGF2BP1 were significantly associated with body size, carcass weight, leg weight, and growth rate. , In a recent study, miR-193b-3p promoted the proliferation of goat skeletal muscle satellite cells by activating IGF2BP1. In addition, IGF2BP1 can promote cancer progression by modulating the mRNA stability of a series of target genes ( IGF2 , ACTB , and CTNNB1 ) . These results suggest that IGF2BP1 is essential for cell proliferation.…”

“…39,40 In a recent study, miR-193b-3p promoted the proliferation of goat skeletal muscle satellite cells by activating IGF2BP1. 41 In addition, IGF2BP1 can promote cancer progression by modulating the mRNA stability of a series of target genes (IGF2, ACTB, and CTNNB1). 42 These results suggest that IGF2BP1 is essential for cell proliferation.…”

Investigation of the Role of Molecules in DNA Repair Process in Coronary Artery Patients

“…Different cell types and different patient groups may be the reason for the difference in the results of the studies. There are many studies in the literature that miR-193b-3p is associated with tumor progression [33,34] and in ammation [9,35]. On the other hand, there are many studies examining the status of this miRNA in cardiovascular diseases.…”