2019
DOI: 10.1186/s12885-018-5245-1
|View full text |Cite
|
Sign up to set email alerts
|

miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα

Abstract: BackgroundMicroRNA-196a-5p (miR-196a-5p) has been reported to be involved in the metastatic process of several cancers. In present work, we aimed to investigate the effects of miR-196a-5p and its potential target IκBα on migration, invasion and epithelial-mesenchymal transition (EMT) of colorectal cancer (CRC) cells.MethodsCCK-8 assay, wound healing assay and cell invasion assay were performed to evaluate the cell proliferation, migration and invasion. In vivo metastasis models were used to investigate the tum… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

3
47
0

Year Published

2019
2019
2024
2024

Publication Types

Select...
6
1
1

Relationship

1
7

Authors

Journals

citations
Cited by 64 publications
(50 citation statements)
references
References 30 publications
3
47
0
Order By: Relevance
“…Cell migration and invasion were analyzed using Transwell assay (pore size: µm) in the 24-well plates as previously described with appropriate improvement [30]. For the migration assay, 5 × 10 4 cells were suspended in serum-free medium and plated in upper chambers (Corning Costar, New York, NY, USA).…”
Section: Cell Migration and Invasion Assaysmentioning
confidence: 99%
“…Cell migration and invasion were analyzed using Transwell assay (pore size: µm) in the 24-well plates as previously described with appropriate improvement [30]. For the migration assay, 5 × 10 4 cells were suspended in serum-free medium and plated in upper chambers (Corning Costar, New York, NY, USA).…”
Section: Cell Migration and Invasion Assaysmentioning
confidence: 99%
“…IκBα is a cellular protein that is related to suppressing the transcription factor of nuclear factor kappa‐B. In addition, IκBα activation is responsible for the malignant progression of cancer cells . As a downstream component of IκBα, IL‐6 is a cytokine that may be potentially carcinogenic.…”
Section: Discussionmentioning
confidence: 99%
“…In addition, IκBα activation is responsible for the malignant progression of cancer cells. 34 As a downstream component of IκBα, IL-6 is a cytokine that may be potentially carcinogenic. IL-6 is playing roles in tumor microenvironment regulation, promoting metastasis by reduction of ECM, and changing tumorous DNA methylation.…”
Section: Discussionmentioning
confidence: 99%
“…The mRNA levels of genes were measured by real-time PCR assay. 18 Briefly, total RNA isolated from cells or tissues was reversely transcribed into cDNAs using Super M-MLV reverse transcriptase (Tiangen, Beijing, China). Real-time PCR was carried out with 2 × Power Taq PCR MasterMix (Tiangen) and SYBR Green (Solarbio, Beijing, China) on an ExiCycler™ 96 Real-time Quantitative Thermal Block (Bioneer Corporation, Daejeon, Korea).…”
Section: Real-time Pcrmentioning
confidence: 99%