miR-197: A novel biomarker for cancers

Wang, Dandan; Chen, Xiu; Yu, Dandan; Yang, Su-Jin; Shen, Huan; Sha, Huanhuan; Zhong, Shanliang; Zhao, Jinyan; Tang, Jinhai

doi:10.1016/j.gene.2016.06.035

Cited by 42 publications

(34 citation statements)

References 82 publications

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“…Another study has reported miR‐520c and miR‐373 as prometastatic, which enhanced the expression of matrix metalloproteinase by targeting sirtuin‐1 and mammalian target of rapamycin in fibrosarcoma . Moreover, among the plethora of miRNAs studied so far in cancers, miR‐197 has been seen to play differential roles, either as a tumor suppressor or oncogene in several tumorigenic pathways, such as cell proliferation, differentiation, apoptosis, angiogenesis, invasion, and metastasis, drug resistance, etc . However, its roles in oncogenesis of fibrosarcoma are not yet explored.…”

Section: Introductionmentioning

confidence: 99%

miR‐197‐5p inhibits sarcomagenesis and induces cellular senescence via repression of KIAA0101

Jain

Roy

Das

et al. 2019

Molecular Carcinogenesis

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The abnormal expressions of microRNAs (miRNAs) are known to be associated with various pathophysiological processes that lead to the development of a plethora of diseases including cancer. Among several miRNAs studied so far, miR-197 has been reported to play a vital role either as an oncogene or tumor suppressor in different cancers. However, its role in carcinogenesis of fibrosarcoma has not yet been elucidated. Therefore, the current study investigated the role of miR-197-5p, which is significantly downregulated in HT1080 fibrosarcoma cells compared to IMR90-tert fibroblast cells. The transient overexpression of miR-197-5p causes a significant decrease in viability and proliferation of fibrosarcoma cells in both concentration-and time-dependent manners. Interestingly, we did not observe any significant changes in cell cycle pattern or apoptotic cell populations, but rather noticed cellular senescence of fibrosarcoma cells upon overexpression of miR-197-5p. Further, this miRNA suppresses the metastatic properties, such as migration, invasion, and anchorageindependent growth of fibrosarcoma possibly through targeting KIAA0101, which is a proliferating cell nuclear antigen-associated factor and overexpressed in the malignancy. In nutshell, our result revealed that miR-197-5p acts as an oncosuppressor miRNA in fibrosarcoma through target regulation of KIAA0101, which can be exploited for developing RNA-based therapeutic strategies for the cure of this malignancy.

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Section: Introductionmentioning

confidence: 99%

miR‐197‐5p inhibits sarcomagenesis and induces cellular senescence via repression of KIAA0101

Jain

Roy

Das

et al. 2019

Molecular Carcinogenesis

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“…MicroRNAs (miRNAs or miRs) are single-stranded noncoding RNAs of 21-23 nucleotides in length (20)(21)(22). miRNAs miR-27a-3p promotes the malignant phenotypes of osteosarcoma by targeting ten-eleven translocation 1 regulate gene expression via the specific binding to target genes (23). Over the past decades, a large number of miRNAs have been identified and are believed to play an important role in the occurrence, differentiation, proliferation and apoptosis of cells (24,25).…”

Section: Introductionmentioning

confidence: 99%

miR-27a-3p promotes the malignant phenotypes of osteosarcoma by targeting ten-eleven translocation 1

Liu

et al. 2018

Int J Oncol

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Osteosarcoma has become one of the most common primary malignant tumors affecting children and adolescents. Although increasing evidence has indicated that microRNAs (miRNAs or miRs) play important roles in the development of osteosarcoma, the expression of miR‑27a‑3p and its effects on osteosarcoma are not yet fully understood. In the present study, our data demonstrated that the expression of miR‑27a‑3p in osteosarcoma cell lines was significantly higher than that in the normal human osteoblastic cell line, hFOB 1.19 cell (P<0.01). In order to explore the role of miR‑27a‑3p in the development and progression of osteosarcoma, the expression of miR‑27a‑3p was inhibited by transfection of the MG-63 cells with miR‑27a‑3p inhibitor. The results revealed that the cell proliferative ability significantly decreased (P<0.01), the number of apoptotic cells significantly increased (P<0.01) and the number of cells passing through the Transwell membrane was significantly reduced in the group transfected with the miR‑27a‑3p inhibitor (P<0.01). At the same time, the expression of E-cadherin and α-catenin was significantly upregulated (P<0.01), while the expression of vimentin was significantly downregulated in the group transfected with the miR‑27a‑3p inhibitor (P<0.01). Our results also revealed that the mRNA expression of ten-eleven translocation 1 (TET1) in the osteosarcoma cells was significantly downregulated compared with that in the hFOB 1.19 cells (P<0.01). Luciferase reporter system analysis indicated that miR‑27a‑3p recognized the TET1 3'-UTR. The protein expression of TET1 significantly increased in the group transfected with the miR‑27a‑3p inhibitor. The results from CCK-8 assay, flow cytometric assay and Transwell invasion analysis revealed that TET1 knockdown inhibited the biological effects induced by the downregulation of miR‑27a‑3p. Taken together, the findings of this study indicate that miR‑27a‑3p is upregulated, while TET1 is downregulated in human osteosarcoma cells. miR‑27a‑3p inhibition suppresses the proliferation and invasion of osteosarcoma cells, and promotes cell apoptosis via the negative regulation of TET1. miR‑27a‑3p/TET1 may thus be a potential target for the treatment of osteosarcoma.

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“…Growing evidence suggests miR-197 as a novel biomarker for various cancers (9). Several studies revealed that miR-197 may be an oncomiR in cancer cell invasion and metastasis.…”

Section: Introductionmentioning

confidence: 99%

miR‑197 promotes the invasion and migration of colorectal cancer by targeting insulin‑like growth factor‑binding protein�3

Zhou

Sun

et al. 2018

Oncol Rep

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The incidence and mortality of colorectal cancer (CRC) have been rising rapidly in China. A number of miRNAs have been confirmed to be involved in diverse biological processes of CRC. However, whether miR‑197 plays a role in migration and invasion of CRC has never been explored. In the present study Transwell chambers were used in in vitro migration and invasion assays. Dual-luciferase reporter assay was employed to confirm the target of miR‑197. RT‑PCR and IHC staining were performed to quantify miR‑197 and IGFBP3 expression, respectively. Clinicopathological features were collected for statistical analysis. We observed that the overexpression of miR‑197 significantly promoted migration and invasion in 3 CRC cell lines including HCT8, HCT116 and SW480 (P<0.05), while the inhibition of miR‑197 weakened both biological processes (P<0.05). In bioinformatics and dual-luciferase reporter assay, luciferase activities of IGFBP3‑WT‑transfected cells significantly decreased upon miR‑197 overexpression and this inhibitory effect was abolished when miR‑197 binding region in IGFBP3 3'‑UTR was mutated, which indicated that miR‑197 directly suppressed the expression of IGFBP3 in CRC cells by targeting its 3'UTR. Downregulation of the expression of IGFBP3 by using targeted siRNA led to significant enhancement of cell migration and invasion in two CRC cell lines including HCT8 and HCT116 (P<0.05). Finally, in cancerous tissues of CRC patients, the miR‑197 level was inversely correlated with the expression of IGFBP3 (P=0.026), which indicated that miR‑197 may modulate cell migration and invasion by targeting IGFBP3 in CRC patients. In conclusion, we revealed that miR‑197 modulates IGFBP3 and therefore plays a critical role in regulating CRC migration and invasion.

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miR-197: A novel biomarker for cancers

Cited by 42 publications

References 82 publications

miR‐197‐5p inhibits sarcomagenesis and induces cellular senescence via repression of KIAA0101

miR‐197‐5p inhibits sarcomagenesis and induces cellular senescence via repression of KIAA0101

miR-27a-3p promotes the malignant phenotypes of osteosarcoma by targeting ten-eleven translocation 1

miR‑197 promotes the invasion and migration of colorectal cancer by targeting insulin‑like growth factor‑binding protein�3

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