miR-199b-5p-DDR1-ERK signalling axis suppresses prostate cancer metastasis via inhibiting epithelial-mesenchymal transition

Zhao, Zhigang; Zhao, Shankun; Luo, Lianmin; Xiang, Qian; Zhu, Zhiguo; Wang, Jiamin; Liu, Yangzhou; Luo, Jin-Yan

doi:10.1038/s41416-020-01187-8

Cited by 33 publications

(26 citation statements)

References 32 publications

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“…Study has found that miR-199b-5p is highly expressed in osteosarcoma and promotes the malignant development of osteosarcoma ( 10 ). However, miR-199b-5p is under-expressed in prostate cancer and breast cancer and inhibits tumor cell growth ( 11 , 12 ). These studies show that miR-199b-5p plays a two-way regulatory role in different cancer cells.…”

Section: Introductionmentioning

confidence: 99%

MiR-199b-5p Promotes Gastric Cancer Progression by Regulating HHIP Expression

Chen

Zhu

et al. 2021

Front. Oncol.

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ObjectivesGastric cancer (GC) is one of the most common malignant tumors. More and more evidences support the role of microRNAs (miRNAs) in tumor progression. However, the role of miRNAs in human GC remains largely unknown.MethodsBased on the published gastric cancer expression profile data, combined with bioinformatics analysis, potential miRNAs in the process of GC were screened. The expression of miR-199b-5p in GC cells and patients’ plasma was detected by RT-PCR. The effects of miR-199b-5p on GC in vitro were detected by EdU proliferation assay, colony formation assay, Transwell assay and wound healing assay. Western blot was used to detect epithelial-mesenchymal transition (EMT) related proteins. The subcutaneous tumorigenesis model and metastatic tumor model of mice were used to study its effect in vivo. Bioinformatics and Dual luciferase reporter assay were used to verify the effect of miR-199b-5p and its target gene.ResultsThrough bioinformatics analysis, we screened a novel miRNA miR-199b-5p that was significantly up-regulated in GC tissue and associated with poor prognosis of GC patients. RT-PCR results showed that its expression was also up-regulated in GC cell lines and patients’ plasma. MiR-199b-5p can significantly promote GC cell proliferation and migration in vitro and in vivo. Western blot showed that miR-199b-5p could promote the EMT process of GC. HHIP has been proved to be a target of miR-199b-5p, and the recovery of HHIP can weaken the effect of miR-199b-5p.ConclusionMiR-199b-5p may play an oncogene role in GC by targeting HHIP, suggesting that miR-199b-5p may be a potential therapeutic target for GC.

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Section: Introductionmentioning

confidence: 99%

MiR-199b-5p Promotes Gastric Cancer Progression by Regulating HHIP Expression

Chen

Zhu

et al. 2021

Front. Oncol.

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“…A group of miRNAs, such as miR-146b, miR-210-3p, miR-636, miR-491-5p, have proved to play the driving role in the growth and metastasis of PCa (6)(7)(8). In addition, our previous studies have confirmed that miR-199b-5p and miR-671-5p can influence the metastasis of PCa by affecting epithelialmesenchymal transition and transcriptional regulation, respectively (9,10). Therefore, on this basis, we will further study the regulatory mechanism of miRNAs in the process of tumor metastasis.…”

Section: Introductionmentioning

confidence: 81%

“…Human PCa cell line C4-2 and human embryonic kidney cell 293 T were obtained from the American Type Culture Collection (Manassas, VA, USA). All cell lines have been authenticated and were cultured as described previously (9).…”

Section: Human Tissue Samples and Cell Linesmentioning

confidence: 99%

“…Protein concentrations were determined using the BCA protein assay regents (#23225, Thermo Pierce, Rockford, IL, USA). The procedure of western blot was conducted as previously described (9). Primary antibodies used in the study include anti-AKAP13 (Immunoway, YT0161; Abcam, ab99377), anti-HEG1 (Biorbyt, orb157480), anti-caspase 3 (Immunoway, YT6113), anti-caspase 3 p17 (Immunoway, YT6161).…”

Section: Protein Extraction and Western Blottingmentioning

confidence: 99%

“…The migration and invasion ability of PCa cells were measured by wound-healing assays and transwell assay, respectively. The detailed procedure was conducted as described in our previous research (9,16). For wound-healing assays, cells were plated into 6-well culture plates and cultured until 100% confluence, then the growth medium was removed, cells were washed and cultured with fresh serum-free medium, and the wound was produced by a 10mL sterile pipette tip.…”

Section: Cell Migration and Invasion Assaysmentioning

confidence: 99%

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MiR-629-5p Promotes Prostate Cancer Development and Metastasis by Targeting AKAP13

et al. 2021

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Prostate cancer (PCa) has become the most frequently occurring cancer among western men according to the latest report, and patients’ prognosis is often poor in the event of tumor progression, therefore, many researches are devoted to exploring the molecular mechanism of PCa metastasis. MicroRNAs (miRNA) have proved to play an important role in this process. In present study, by combining clinical samples with public databases, we found that miR-629-5p increased to varying degrees in primary localized PCa tissues and metastatic PCa tissues compared with adjacent normal tissues, and bioinformatics analysis suggested that high level of miR-629-5p was related to poor prognosis. Functionally, miR-629-5p drove PCa cell proliferation, migration and invasion in vitro, and promoted growth of PCa cells in vivo. Moreover, A-kinase Anchor Protein 13 (AKAP13) was screened as a direct target of miR-629-5p, that expression was negatively correlated with the malignant phenotype of tumor cells. In the end, through verification in clinical specimens, we found that AKAP13 could be independently used as a clinical prognostic indicator. Overall, the present study indicates that miR-629-5p plays an oncogenic role in PCa by targeting AKAP13, which provides a new idea for clinical diagnosis and treatment of complex refractory PCa.

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CircTNPO3 promotes hepatocellular carcinoma progression by sponging miR‐199b‐5p and regulating STRN expression

Liu

2022

The Kaohsiung J of Med Scie

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Hepatocellular carcinoma (HCC) is the most common primary liver tumor, which seriously threatens human health. CircTNPO3 was up‐regulated in HCC tissues. However, the regulatory mechanism of circTNPO3 in HCC was still unclear. We aimed to investigate the circTNPO3 function in the development of HCC. qRT‐PCR and Western blot examined gene and protein levels. CCK8, EdU, flow cytometry, and Transwell assays were used to detect cell viability, proliferation, apoptosis, and invasion abilities. Dual‐luciferase reporter and RIP assays determined the relationship between circTNPO3, miR‐199b‐5p, and striatin (STRN). The effect of CircTNPO3 on HCC progress was investigated in vivo. CircTNPO3 and STRN were significantly increased, while miR‐199b‐5p was repressed in HCC tissues or cells. Afterward, miR‐199b‐5p was negatively correlated with STRN. circTNPO3 was positively correlated with STRN. Knockdown of circTNPO3 inhibited cell viability, proliferation, invasion, and promoted apoptosis, while circTNPO3 overexpression had the opposite results. Furthermore, miR‐199b‐5p inhibition could eliminate the regulatory effect of sh‐circTNPO3 on the proliferation and apoptosis in HCC cells. CircTNPO3 positively regulated STRN expression by targeting miR‐199b‐5p. MiR‐199b‐5p suppressed HCC progression by inhibiting STRN expression. Tumor formation in nude mice showed that knockdown of circTNPO3 significantly inhibited tumor growth and suppressed ki‐67 levels. CircTNPO3 promoted HCC progression through regulating STRN expression by sponging miR‐199b‐5p, which provided a strategy for HCC treatment.

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miR-199b-5p-DDR1-ERK signalling axis suppresses prostate cancer metastasis via inhibiting epithelial-mesenchymal transition

Cited by 33 publications

References 32 publications

MiR-199b-5p Promotes Gastric Cancer Progression by Regulating HHIP Expression

MiR-199b-5p Promotes Gastric Cancer Progression by Regulating HHIP Expression

MiR-629-5p Promotes Prostate Cancer Development and Metastasis by Targeting AKAP13

CircTNPO3 promotes hepatocellular carcinoma progression by sponging miR‐199b‐5p and regulating STRN expression

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