miR-19a-3p inhibition alleviates sepsis-induced lung injury via enhancing USP13 expression

Ren, Hangqi; Mu, Wei; Xu, Qiaolian

doi:10.18388/abp.2020_5505

Cited by 9 publications

(8 citation statements)

References 21 publications

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“…Herein, circ_0001498 level upregulation was detected in sepsis-related ALI patients. LPS has been used to establish sepsis-induced ALI cell model (Ren et al 2021;. Also, our experimental results demonstrated that cell viability was suppressed but apoptosis and inflammatory factors were stimulated by LPS in WI-38 cells.…”

Section: Discussionmentioning

confidence: 59%

Circ_0001498 contributes to lipopolysaccharide-induced lung cell apoptosis and inflammation in sepsis-related acute lung injury via upregulating SOX6 by interacting with miR-574-5p

Hu¹,

Wang²,

Luo³

et al. 2023

gpb

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Circular RNAs (circRNAs) have important regulation in in sepsis-related acute lung injury (ALI). Circ_0001498 was significantly overexpressed in sepsis-induced acute respiratory distress syndrome. The aims of this study were to explore role and mechanism of circ_0001498 in lipopolysaccharide (LPS)-treated WI-38 cells. Human samples were collected from 56 sepsis patients and 46 healthy volunteers at Liyang People's Hospital. Circ_0001498, microRNA-574-5p (miR-574-5p) or sex-determining region Y-related high-mobility-group box 6 (SOX6) levels were detected via reverse transcription-quantitative polymerase chain reaction assay. Cell viability was determined through Cell Counting Kit-8 assay. Apoptosis rate was examined by flow cytometry. Western blot was used for measurement of proteins. Inflammatory cytokines were detected via enzyme-linked immunosorbent assay. Target relation was analyzed via dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Circ_0001498 was overexpressed in sepsisrelated ALI patients and LPS-treated WI-38 cells. Silencing circ_0001498 reduced LPS-induced cell apoptosis and inflammation. Circ_0001498 interacted with miR-574-5p. The regulation of circ_0001498 knockdown was abolished by miR-574-5p inhibitor. Furthermore, miR-574-5p directly targeted SOX6 and circ_0001498 upregulated SOX6 via targeting miR-574-5p. Overexpression of miR-574-5p alleviated LPS-induced cell injury by downregulating SOX6. This research identified that circ_0001498 facilitated sepsis-related ALI progression by targeting miR-574-5p to upregulate SOX6.

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Section: Discussionmentioning

confidence: 59%

Circ_0001498 contributes to lipopolysaccharide-induced lung cell apoptosis and inflammation in sepsis-related acute lung injury via upregulating SOX6 by interacting with miR-574-5p

Hu¹,

Wang²,

Luo³

et al. 2023

gpb

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“…The downregulation of mir-19a-3p was found to improve inflammation and ALI induced by sepsis in both in vivo and in vitro experiments, as the reduction in pro-inflammatory cytokines demonstrated. It seems that the target gene of miR-19a-3p is represented by USP13, whose over-expression is related to amelioration and better outcomes of lung injury [ 200 ].…”

Section: Resultsmentioning

confidence: 99%

Expression of MicroRNAs in Sepsis-Related Organ Dysfunction: A Systematic Review

Maiese

Scatena

Costantino

et al. 2022

IJMS

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Sepsis is a critical condition characterized by increased levels of pro-inflammatory cytokines and proliferating cells such as neutrophils and macrophages in response to microbial pathogens. Such processes lead to an abnormal inflammatory response and multi-organ failure. MicroRNAs (miRNA) are single-stranded non-coding RNAs with the function of gene regulation. This means that miRNAs are involved in multiple intracellular pathways and thus contribute to or inhibit inflammation. As a result, their variable expression in different tissues and organs may play a key role in regulating the pathophysiological events of sepsis. Thanks to this property, miRNAs may serve as potential diagnostic and prognostic biomarkers in such life-threatening events. In this narrative review, we collect the results of recent studies on the expression of miRNAs in heart, blood, lung, liver, brain, and kidney during sepsis and the molecular processes in which they are involved. In reviewing the literature, we find at least 122 miRNAs and signaling pathways involved in sepsis-related organ dysfunction. This may help clinicians to detect, prevent, and treat sepsis-related organ failures early, although further studies are needed to deepen the knowledge of their potential contribution.

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“…MiRNAs are short segments of noncoding molecules with ~22 nucleotides that can regulate cellular events by post-transcriptionally modulating their mRNAs (26). In addition, altered miRNAs has been pointed out to have roles in regulating the process of sepsis-ALI (27)(28)(29). Wang and Han (30) showed that miR-1184 was decreased in the serum of neonatal sepsis, and overexpression of miR-1184 targetedly suppressed IL-6 level to inhibit LPS-stimulated inflammatory reaction in monocytes.…”

Section: Discussionmentioning

confidence: 99%

Circ_0033530 Knockdown Alleviates Lipopolysaccharide-Induced Acute Lung Injury Model of Human Lung Fibroblasts by Mir-1184/Tlr4 Axis

Xia,

Shan,

Luo

et al. 2023

Shock

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Background Circular RNAs (circRNAs) have been reported to be involved in regulating the progression of sepsis and sepsis-associated damage. Herein, this work investigated whether circ_0033530 had roles in the process of septic acute lung injury (sepsis-ALI) and its associated mechanism. Methods Lipopolysaccharide (LPS)-stimulated human lung fibroblasts MRC-5 were used to mimic the cell model of sepsis-ALI in vitro. Levels of genes and proteins were detected by qRT-PCR and western blotting. Functional experiments were conducted using 5-ethynyl-2’-deoxyuridine (EdU) assay, Cell counting Kit-8 (CCK-8) assay, flow cytometry, and Enzyme-Linked Immunosorbent Assay (ELISA), respectively. The interaction between miR-1184 and circ_0033530 or Toll-like receptor 4 (TLR4) was confirmed by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Results Circ_0033530 expression was lower in sepsis patients and LSP-induced fibroblasts than those in healthy control and untreated cells. Functionally, knockdown of circ_0033530 protected fibroblasts against LPS-induced proliferation arrest, apoptosis and inflammatory response. Mechanistically, circ_0033530 acted as a sponge for miR-1184, and TLR4 RNA was targeted by miR-1184, indicating the circ_0033530/miR-1184/TLR4 axis. Further rescue experiments showed that circ_0033530 silencing-mediated growth inhibition and inflammation on fibroblasts were attenuated by miR-1184 down-regulation or TLR4 up-regulation. Conclusion Circ_0033530 knockdown alleviated LPS-induced proliferation arrest, apoptosis and inflammation in lung fibroblasts by miR-1184/TLR4 axis, provide molecular theoretical basis for circ_0033530 on the pathogenesis of sepsis-ALI.

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miR-19a-3p inhibition alleviates sepsis-induced lung injury via enhancing USP13 expression

Cited by 9 publications

References 21 publications

Circ_0001498 contributes to lipopolysaccharide-induced lung cell apoptosis and inflammation in sepsis-related acute lung injury via upregulating SOX6 by interacting with miR-574-5p

Circ_0001498 contributes to lipopolysaccharide-induced lung cell apoptosis and inflammation in sepsis-related acute lung injury via upregulating SOX6 by interacting with miR-574-5p

Expression of MicroRNAs in Sepsis-Related Organ Dysfunction: A Systematic Review

Circ_0033530 Knockdown Alleviates Lipopolysaccharide-Induced Acute Lung Injury Model of Human Lung Fibroblasts by Mir-1184/Tlr4 Axis

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