MiR-19b-3p attenuates IL-1β induced extracellular matrix degradation and inflammatory injury in chondrocytes by targeting GRK6

Duan, Liang; Duan, Dapeng; Wei, Wensheng; Sun, Zhengming; Xu, Huiyong; Guo, Lipeng; Wu, Xuanhui

doi:10.1007/s11010-019-03563-2

Cited by 34 publications

(24 citation statements)

References 27 publications

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“…35 MiR-19b-3p was also reported to attenuate IL-1β-induced inflammatory injury in chondrocytes through targeting GRK6. 16 As for miR-1281, it has been identified as a tumour suppressor in many malignancies such as glioma, 17 osteosarcoma 36 and gastric cancer. 37 Interestingly, it also presented protective functions in macrophages against Mtb infection by reducing the programmed necrosis and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Phosphorylated STAT3 suppresses microRNA‐19b/1281 to aggravate lung injury in mice with type 2 diabetes mellitus‐associated pulmonary tuberculosis

Wang¹,

Lin

Liang

et al. 2020

J Cellular Molecular Medi

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Section: Discussionmentioning

confidence: 99%

Phosphorylated STAT3 suppresses microRNA‐19b/1281 to aggravate lung injury in mice with type 2 diabetes mellitus‐associated pulmonary tuberculosis

Wang¹,

Lin

Liang

et al. 2020

J Cellular Molecular Medi

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“…A previous study reported that miRNA-17-5p was significantly downregulated in OA chondrocytes and facilitated autophagy by decreasing p62 expression ( 32 ). Additionally, miRNA-19b-3p decreases IL-1β-induced ECM degradation and inflammatory injury in chondrocytes by regulating G protein-coupled receptor kinase 6 expression ( 33 ). Similar to previous studies, the results of the present study indicated that miR-17-5p expression was decreased in OA chondrocytes compared with that in the control cells.…”

Section: Discussionmentioning

confidence: 99%

miR‑17‑5p and miR‑19b‑3p prevent osteoarthritis progression by targeting EZH2

Yuan

Song

et al. 2020

Exp Ther Med

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Osteoarthritis (OA) is a joint disease caused by a variety of factors, including aging, obesity and trauma. MicroRNAs (miRNAs) have been reported to be crucial regulators during OA progression. The present study aimed to investigate the role of miR-17-5p and miR-19b-3p during OA development. Interleukin (IL)-1β-treated chondrocytes were used to mimic OA in vitro. The expression levels of miR-17-5p and enhancer of zeste homolog 2 (EZH2) were measured in cartilage tissues and chondrocytes using reverse transcription-quantitative PCR or western blotting. Apoptosis was assessed by flow cytometry. The protein expression levels of extracellular matrix (ECM)-associated genes were detected by western blotting. The binding sites between miR-17-5p or miR-19b-3p and EZH2 were predicted using the MicroT-CDS online database and verified using dual-luciferase reporter and RIP assays. miR-17-5p expression was downregulated, whereas EZH2 expression was upregulated in OA cartilage tissues and IL-1β-induced chondrocytes compared with that in the control tissues and cells. miR-17-5p mimics inhibited IL-1β-induced apoptosis and ECM degradation in chondrocytes. EZH2 was the target of miR-17-5p and miR-19b-3p in chondrocytes, and enhanced apoptosis and ECM degradation in IL-1β-stimulated chondrocytes. Rescue experiments revealed that miR-17-5p or miR-19b-3p mimic-induced inhibition of OA progression was reversed by EZH2 overexpression. In conclusion, miR-17-5p and miR-19b-3p inhibited OA progression by targeting EZH2, which may serve as a potential therapeutic target for OA.

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“…Gantier et al first reported that miR-19b regulated the activity of nuclear factor-kB (NF-kB) signaling in inflammation (42). Over-expression of miR-19b-3p inhibited the production of IL-6 and IL-8, and the interaction of miR-19b-3p with its direct target gene, G protein-coupled receptor kinase 6 (GRK6), was discovered to affect inflammation (43). TNFAIP3, which is negatively regulated by miR-19b-3p, is widely recognized as an important regulator of inflammation (44).…”

Section: Discussionmentioning

confidence: 99%

Plasma microRNA Array Analysis Identifies Overexpressed miR-19b-3p as a Biomarker of Bipolar Depression Distinguishing From Unipolar Depression

et al. 2020

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Objectives: The clinical characteristics of bipolar disorder (current major depressive episode) (BD) overlap with unipolar depressive disorder (UD), which makes it difficult to perform an accurate diagnosis. We identified plasma microRNAs (miRNAs) that distinguished BD from UD and explored the relationship between miRNA expression levels and clinical characteristics. Methods: Total miRNAs from blood plasma from seven UD patients, seven BD patients, and six controls were analyzed. The identified miRNAs were validated in a separate population group. Depression severity and early life adversities were assessed. Bioinformatic analysis was conducted to investigate the target genes that were identified and the pathways associated with the altered miRNAs. Results: Compared to controls, 42 miRNAs were differentially expressed in patients. miR-19b-3p, miR-3921, and miR-1180-3p were selected to validate the microarray results. Only miR-19b-3p was validated as down-regulated in patients. The primary predicted genes associated with miR-19b-3p were MAPK1, PTEN, and PRKAA1. The most relevant KEGG pathways included mTOR, FoxO, and the PI3-K/Akt signaling pathway. BD patients were more likely to have higher expression levels of miR-19b-3p and more severe childhood trauma experience compared to UD patients. Conclusions: Plasma miR-19b-3p is a potential non-invasive biomarker that might be useful in distinguishing UD from BD. miR-19b3p was predicted to be involved in the pathway of inflammatory dysregulation associated with experiencing early childhood trauma.

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MiR-19b-3p attenuates IL-1β induced extracellular matrix degradation and inflammatory injury in chondrocytes by targeting GRK6

Cited by 34 publications

References 27 publications

Phosphorylated STAT3 suppresses microRNA‐19b/1281 to aggravate lung injury in mice with type 2 diabetes mellitus‐associated pulmonary tuberculosis

Phosphorylated STAT3 suppresses microRNA‐19b/1281 to aggravate lung injury in mice with type 2 diabetes mellitus‐associated pulmonary tuberculosis

miR‑17‑5p and miR‑19b‑3p prevent osteoarthritis progression by targeting EZH2

Plasma microRNA Array Analysis Identifies Overexpressed miR-19b-3p as a Biomarker of Bipolar Depression Distinguishing From Unipolar Depression

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