MiR-200c inhibits bladder cancer progression by targeting lactate dehydrogenase A

Yuan, Daozhang; Zheng, Shunsheng; Wang, Liyan; Li, Jing; Yang, Jianan; Wang, Bin; Chen, Xiong; Zhang, Xiaobo

doi:10.18632/oncotarget.18801

Cited by 29 publications

(22 citation statements)

References 28 publications

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“…22 It has been shown that the expression of miRNA is changed in cancer via several mechanisms such as miRNA gene deletion or amplification. 25 Zhang et al 26 reported that miR-200c suppressed the breast cancer cell metastasis via downregulation of forkhead box F2 expression. Individual miRNAs can regulate multiple mRNAs, and several distinct miRNAs can suppress a single mRNA.…”

Section: Discussionmentioning

confidence: 99%

Retracted: Anticancer effects of miR‐200c in colorectal cancer through BMI1

Mazraehshah

Tavangar

Saidijam

et al. 2018

J of Cellular Biochemistry

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Colorectal cancer (CRC) is one of the most leading cancer deaths throughout the world. MiR-200c has been shown to have a critical role in cancer initiation and progression. In this study, we investigated the miR-200c expression in CRC tissues and its effects on CRC cell lines which were mediated by polycomb complex protein (BMI1). Quantitative reverse transcription polymerase chain reaction (QRT-PCR) and immunohistochemistry were used to detect miR-200c and BMI1 expression in tumor tissues from 38 patients with CRC and 38 normal colon tissues. HCT-116 and SW-48 cells were transfected by locked nucleic acid (LNA)-anti-miR-200c. Western blot analysis and real-time PCR were applied to determine the BMI1 protein and microRNA (miRNA) levels. The apoptosis was analyzed via annexin/propidium iodide staining, and cell invasion was evaluated by transwell assay. MiR-200c was markedly downregulated in CRC tissues, whereas the protein expression of BMI1 in CRC tissues was upregulated compared with normal colon tissues. In the colon cancer cell lines, transfection of LNA-anti-miR-200c increased BMI1 gene and protein expression as well as the cell invasion. Downregulation of miR-200c by LNA decreased the apoptotic cells. The results from this study revealed that miR-200c may have antitumor effects through inhibition of BMI1 expression.

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Section: Discussionmentioning

confidence: 99%

Retracted: Anticancer effects of miR‐200c in colorectal cancer through BMI1

Mazraehshah

Tavangar

Saidijam

et al. 2018

J of Cellular Biochemistry

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“…In this present study, knockdown of LDHA expression using LDHA targeting siRNA significantly suppressed the proliferation and migration of A549 and NCI-H460 NSCLC cells, similar to the effects observed following miR-200c overexpression. In addition, LDHA has been identified as a direct target of miR-200c in bladder cancer 29 . Therefore, the present study provides further evidence regarding the relationship between miR-200c and LDHA in human cancer.…”

Section: Discussionmentioning

confidence: 99%

The Downregulation of miR-200c Promotes Lactate Dehydrogenase A Expression and Non-Small Cell Lung Cancer Progression

et al. 2018

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This study was aimed to investigate the function and mechanism of microRNA-200c (miR-200c) in the progression of non-small cell lung cancer (NSCLC). A total of 76 patients diagnosed as having NSCLC were enrolled in this study. The expression level of miR-200c in NSCLC tissues and cell lines was investigated using the quantitative real-time polymerase chain reaction (RT-qPCR) method. We found that the expression of miR-200c was significantly reduced in NSCLC tissues and cell lines compared with normal lung tissues and the human bronchial epithelial cell line. Overexpression of miR-200c using the miR-200c mimic significantly suppressed cell proliferation and migration of NSCLC cell lines. The results of the luciferase reporter assay identified lactate dehydrogenase A (LDHA) as a direct target of miR-200c. The expression of LDHA was shown to be suppressed in NSCLC cell lines with miR-200c mimic transfection. Furthermore, the transfection of small interfering RNA (siRNA) targeting LDHA suppressed the proliferation and migration of NSCLC cell lines. In summary, our results presented in this study suggested that miR-200c was able to inhibit the proliferation and migration of NSCLC cells by downregulating LDHA. Therefore, miR-200c may be considered as a potential candidate for the treatment of NSCLC.

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“…MiR-200c is a well-known tumor suppressor, and its downregulation has been observed in various cancers, such as breast cancer, gastric cancer, etc [16,17]. In bladder cancer, it has been reported that the ectopic expression of miR-200c could inhibit bladder cancer cell proliferation and invasion in vitro [18]. MiR-200c may be involved in tumorigenesis of bladder cancer, which may be employed as an indicator for early screening of the malignant disease.…”

Section: Introductionmentioning

confidence: 99%

Downregulation of Urinary microRNA-200c acts as a Promising Novel Bio-marker in the Diagnosis of Patients With Bladder Cancer

Zi¹,

Tao²,

Gao³

et al. 2020

Preprint

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BackgroundMicroRANs (miRNAs) have been reported to be involved in various human cancers. The aim of this study was to explore the diagnostic performance of urine miR-200c in bladder cancer. MethodsQuantitative real-time polymerase chain reaction (qRT-PCR) method was applied to measure the relative expression of urine miR-200c in bladder cancer patients. The relationship between urine miR-200c level and clinicopathological factors was analyzed using χ2 test. The diagnostic capacity of urine miR-200c was calculated using the receiver operating characteristics (ROC) curve analysis.ResultsUrinary level of miR-200c was significantly reduced in bladder cancer patients compared with healthy controls (P=0.000). Furthermore, urine miR-200c expression was strongly correlated with histologic grade (P=0.019), tumor grade (P=0.003), and lymph node metastasis (P=0.001). ROC curve showed that urine miR-200c could distinguish bladder cancer patients from healthy controls with an area under the curve of 0.844. The cutoff value of 1.235, with the sensitivity of 89.0% and the specificity of 70.7% respectively.ConclusionUrine miR-200c may act as a noninvasive diagnostic biomarker for bladder cancer.

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MiR-200c inhibits bladder cancer progression by targeting lactate dehydrogenase A

Cited by 29 publications

References 28 publications

Retracted: Anticancer effects of miR‐200c in colorectal cancer through BMI1

Retracted: Anticancer effects of miR‐200c in colorectal cancer through BMI1

The Downregulation of miR-200c Promotes Lactate Dehydrogenase A Expression and Non-Small Cell Lung Cancer Progression

Downregulation of Urinary microRNA-200c acts as a Promising Novel Bio-marker in the Diagnosis of Patients With Bladder Cancer

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