miR-205 regulation of ICT1 has an oncogenic potential via promoting the migration and invasion of gastric cancer cells

Tao, Youmao; Song, Yan; Han, Tao; Wang, Changjing; Zhao, Tongquan; Gu, Yinmin

doi:10.1016/j.biopha.2017.09.147

Cited by 14 publications

(13 citation statements)

References 20 publications

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“…Cell scratch assay is a simple and rapid method to measure cell migration and repair ability [31]. As shown in Figure 3, compared with the control group, the cell migration ability of the low, middle, and high dose groups decreased significantly (*** p < 0.001).…”

Section: Resultsmentioning

confidence: 99%

Essential Oil from Pinus Koraiensis Pinecones Inhibits Gastric Cancer Cells via the HIPPO/YAP Signaling Pathway

et al. 2019

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Pinecone is a traditional folk herb, which has been used in China for many years. In this paper, the essential oil from Pinus koraiensis pinecones (PEO) was obtained by hydrodistillation and 41 compounds were identified by gas chromatography–mass spectrometry (GC-MS), mainly including α-Pinene (40.91%), Limonene (24.82%), and β-Pinene (7.04%). The purpose of this study was to investigate the anti-tumor activity of PEO on MGC-803 cells and its mechanism. Anti-tumor experiments in vitro showed PEO could significantly inhibit the proliferation and migration of MGC-803 cells, and it also could arrest the cell cycle in the G2/M phase, decrease the mitochondrial membrane potential, and induce apoptosis. Finally, the effects of PEO on genes expression on MGC-803 cells were analyzed by RNA sequencing, and results showed that after treatment with PEO, 100 genes were up-regulated, and 57 genes were down-regulated. According to the KEGG pathway and GSEA, FAT4, STK3, LATS2, YAP1, and AJUBA were down-regulated, which were related to HIPPO signaling pathway. Real-time PCR and western blot further confirmed the results of RNA sequencing. These results indicated that PEO may exert anti-tumor activity via the HIPPO/YAP signaling pathway. The anti-tumor mechanism of this oil can be further studied, which is important for the development of anti-tumor drugs.

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Section: Resultsmentioning

confidence: 99%

Essential Oil from Pinus Koraiensis Pinecones Inhibits Gastric Cancer Cells via the HIPPO/YAP Signaling Pathway

et al. 2019

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“…In addition, ICT1 knockdown restrained cell growth through causing of cell cycle arrest and apoptosis in the breast cancer [9]. A recent study revealed that ICT1 was upregulated in gastric cancer and promoted its invasion and migration [8]. Whereas, the expression and biological effects of ICT1 in OS has not been studied.…”

Section: Discussionmentioning

confidence: 98%

ICT1 Knockdown inhibits osteosarcoma cell proliferation by regulating cell apoptosis through targeting BCL-2

Pan¹,

Tan²,

Du³

et al. 2020

Preprint

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Background Osteosarcoma (OS) which occurs mainly in the young people is a common malignant bone tumor. Apoptosis is a form of programmed and controlled cell death and promoting apoptosis of OS cells is an important treatment for OS. However, the pathways of controlling apoptosis in OS remain unclear. Immature colon carcinoma transcript-1 (ICT1) which is previously named DS-1 belongs to a family of four putative mitochondrial translation release factors. It controls the termination stage of translation. Methods In this study, the function of ICT1 was elucidated by a series of in vivo and in vitro assays. The potential downstream targets of ICT1 in the OS was identified by qRT-PCR and western blotting, while immunohistochemical analysis and rescue experiment were performed to confirm this result. Results ICT1 was upregulated in OS, and higher expression of ICT1 was associated with worse overall survival rate. Functional studies suggested that knockdown of ICT1 could inhibit OS cell proliferation in vitro or in vivo. In addition, suppression of ICT1 could promote apoptosis of OS cells. Furthermore, we demonstrated that BCL-2, apoptotic related-genes, is a potential target of ICT1 and BCL-2 overexpression partly reversed the inhibitory effects of ICT1 knockdown on the pro-tumorigenic properties of OS cells in vitro. Conclusion The ICT1 protein was overexpressed in the OS and has identified a novel mechanism by which ICT1 inhibits osteosarcoma cell proliferation by regulating cell apoptosis through targeting BCL-2.

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“…Among them, miRNAs is a specific class of ‘small noncoding RNA’ which can alter expression of oncogenes and tumor suppressor genes as well as disrupt cellular functions via binding to the 3′-UTR with target genes 30,31. Deregulation of miR-205 has been reported to be present in gastric cancer, prostate cancer, pancreatic cancer, and even breast tumor 25,26,32,33. Gain-of-function of miR-205 restores chemosensitivity of breast cancer cells to chemotherapy (doxorubicin and taxol) by suppressing both vascular endothelial growth factor A and fibroblast growth factor 2 27.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of miR-205 contributes to epithelial–mesenchymal transition and invasion in triple-negative breast cancer by targeting HMGB1–RAGE signaling pathway

Wang¹,

Kang

Wang

et al. 2019

Anti-Cancer Drugs

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Our aim was to study the regulatory molecule networks involved in the epithelial-to-mesenchymal transition and thus promoting the early onset of metastasis in triple-negative breast cancer (TNBC). Forty pairs of human TNBC and their adjacent normal breast tissues were analyzed by real-time PCR and immunochemistry to demonstrate the correlation between the miR-205 expression and clinicopathological characteristics. In vitro, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay, cell migration, and invasion assay were used to detect the cell growth and invasive ability of TNBC cells after upregulation or downregulation of miR-205 expression. Luciferase reporter assay was used to confirm the potential target directly influenced by miR-205. Our results showed that miR-205 abnormal expression may be involved and associated with the biological traits of TNBC. Ectopic expression of miR-205 not only inhibited cell growth, but also suppressed migration and invasion of mesenchymal-like TNBC cells. In addition, we found that overexpression of miR-205 significantly suppressed HMGB1 by binding its 3′-untranslated region, and that miR-205 was inversely correlated with the expression of HMGB1 and RAGE in cell lines and clinical samples. Our study illustrated that miR-205 was a tumor suppressor in TNBC, which attenuated the viability and the acquisition of the epithelial-to-mesenchymal transition phenotype TNBC cells at least partially exerted through targeting of HMGB1–RAGE signaling pathway.

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miR-205 regulation of ICT1 has an oncogenic potential via promoting the migration and invasion of gastric cancer cells

Cited by 14 publications

References 20 publications

Essential Oil from Pinus Koraiensis Pinecones Inhibits Gastric Cancer Cells via the HIPPO/YAP Signaling Pathway

Essential Oil from Pinus Koraiensis Pinecones Inhibits Gastric Cancer Cells via the HIPPO/YAP Signaling Pathway

ICT1 Knockdown inhibits osteosarcoma cell proliferation by regulating cell apoptosis through targeting BCL-2

Downregulation of miR-205 contributes to epithelial–mesenchymal transition and invasion in triple-negative breast cancer by targeting HMGB1–RAGE signaling pathway

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