miR‑205 suppresses cell migration, invasion and EMT of colon cancer by targeting mouse double minute 4

Abstract

colon cancer is one of the most frequent malignant tumors, and microrna (mir)-205 is involved in the tumor progression. The present study aimed to explore the effects of mir-205 on human colon cancer and its targeting mechanism. The levels of mir-205 and mouse double minute 4 (MdM4) were determined via reverse transcription-quantitative (rT-q) Pcr and western blot analysis. a luciferase activity assay was performed to analyze the association between mir-205 and MdM4. cell viability, migration and invasion were determined via cell counting Kit-8, wound healing and Transwell assays, respectively. The levels of epithelial-mesenchymal transition (eMT)-associated factors were determined by rT-qPcr and western blot analysis. It was identified that MDM4 was overexpressed in colon cancer tissues and cells, and that there was a negative correlation between mir-205 and MdM4 expression in colon cancer. Similarly, mir-205 inhibited MdM4 expression by binding to its 3'untranslated region. in addition, mir-205 directly targeted MdM4, accompanied by suppressed proliferation, migration and invasion of HcT116 cells. eMT processes were suppressed in mir-205-overexpressed cells; upregulation of e-cadherin, and downregulation of n-cadherin, vimentin, matrix metalloproteinase (MMP)2 and MMP9 were observed. collectively, mir-205 conspicuously depressed the viability, migration, invasion and eMT process of human colon cancer cells via targeting MdM4. mir-205 could be potentially used in the treatment of human colon cancer.

The Impact of Non-coding RNAs in the Epithelial to Mesenchymal Transition

“…Notably, this miRNA has also reduced expressions of EMT markers including Snail-1, E-cadherin and VEGF as well as some other oncogenic proteins namely SMAD3 and AKT ( Mansoori et al, 2020 ). In this type of cancer, miR-145-5p, miR-383-5p, miR-3622a-3p, miR-205 and miR-200b inhibit EMT process through targeting CDCA3, SGK1, SALL4, MDM4 and HIF-1α, respectively ( Shang et al, 2017 ; Chong et al, 2019 ; Chang et al, 2020 ; Chen et al, 2020 ; Fan and Wang, 2020 ).…”

Clinical impact of epithelial–mesenchymal transition for cancer therapy

“…88,89 miR-200 has a recent role in inhibiting EMT. [90][91][92][93][94] In breast cancer, miRNA-221 and miRNA-222 are associated with EMT and metastasis. [95][96][97] Several studies have shown that miRNAs can suppress EMT by targeting the TFs involved in Wnt signalling or its downstream targets.…”

“…miRNA‐194 involves multiple complex signalling pathways and MMP protein expression as well as EMT 88,89 . miR‐200 has a recent role in inhibiting EMT 90–94 . In breast cancer, miRNA‐221 and miRNA‐222 are associated with EMT and metastasis 95–97 .…”

MicroRNAs in Epithelial–Mesenchymal Transition Process of Cancer: Potential Targets for Chemotherapy

“…Advanced study demonstrated that overexpressed miR-25 inhibited proliferation, migration, and invasion of HCT116 cells. Additionally, the decreased N-cadherin, vimentin, MMP2, and MMP9 with the increase of E-cadherin were observed in miR-205-overexpressed cells [ 64 ]. MiR-145-5p were down-regulated in CRC samples and cell lines.…”