MiR-206 Target Prediction in Breast Cancer Subtypes by Bioinformatics Tools

Abstract

Background: MicroRNAs (miRNAs) are small endogenous non-coding RNAs with fundamental roles in the regulation of protein expression that is involved in the pathogenesis of many cancers including breast cancer. Among them is miR-206, whose role as a tumor suppressor gene has been demonstrated in breast cancer. Consequently, the identification of its putative target in breast cancer is of practical value. Methods: In the present study, we have suggested a new approach for the identification of miR-206 target genes with possible role in breast cancer pathogenesis. We used 15 online tools for the prediction of miR-206 target genes as well as gene expression data produced by DNA microarray technology. Results: By combining these two sets of data, we suggested a list of miR-206 target genes with possible involvement in breast cancer. In addition, we depicted an interaction network including miR-206 and its putative targets. Conclusions: Considering the complexity of miR-206 interactions with several targets, such in silico analyses would considerably lessen the work load of laboratory experiments.

Early satellite cell communication creates a permissive environment for long-term muscle growth

“…p < 0.05), which points to accelerated progression of a fibrogenic program in FAPs when satellite cells are absent; this was especially apparent in the FAP cluster most enriched with Pdgfra (Figure 5A). In MPC EVs, miR-206 is the most abundant miRNA Murach et al, 2020c), and several bioinformatic analyses in the context of cancer (Seifi-Alan et al, 2018), as well as murine RNA hybrid analysis (DG À21.7 for binding) (Krü ger and Rehmsmeier, 2006), indicated that Wisp1 could be targeted by miR-206. We reasoned that EV delivery of miR-206 could explain lower Wisp1 in FAPs during MOV in the presence of satellite cells in our scRNA-seq experiment.…”

Investigating Unique Genes of Five Molecular Subtypes of Breast Cancer Using Penalized Logistic Regression

“…ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3) is a protein involved in glycan degradation which is a sign of tumor malignancy [27]. Seifi-Alan et al has shown differential expression of EDEM3 in Luminal A subtype [28]. Our model highlighted EDEM3 in Luminal A-Basal comparative group.…”