miR-21-5p is associated with the regulation of estradiol benzoate and oxytocin induced primary dysmenorrhea in rat uterus: a bioinformatic study

Zhao, Ruili; Shi, Ke; Fan, Pei

doi:10.1007/s13258-017-0591-y

Cited by 3 publications

(4 citation statements)

References 40 publications

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“…PD has complicated regulatory mechanisms. Our previous study revealed that several essential pathways involving in smooth muscle contraction were significantly enriched by the DEGs in the uterus of PD syndrome rats 9 . Thereafter, in this study, PPI network analysis of the DEGs were performed to disclose the relations between the cascades and the genes.…”

Section: Discussionmentioning

confidence: 93%

“…The DEGs in the uterus of PD syndrome rat were shown in our previously published paper 9 . In brief, 682 DEGs, including 267 up-regulated and 415 down-regulated genes, were identified from the total RNA samples of the rat uterus with PD syndrome induced by estradiol benzoate and oxytocin via an Illumina NextSeq 500, 2 × 150 approach.…”

Section: Methodsmentioning

confidence: 95%

“…The data showed that, in the uterus of PD syndrome rats, 267 and 415 mRNAs were up- and down-regulated, respectively. These differentially expressed genes (DEGs) were significantly enriched in a series of pathways in rat uterus that are associated with the USMC functionalities 9 . It revealed that some essential biological processes, such as cellular response to calcium ion and SMAD protein signaling transduction, were fortified in the uterus of PD syndrome rats.…”

Section: Introductionmentioning

confidence: 99%

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The PPI network analysis of mRNA expression profile of uterus from primary dysmenorrheal rats

Fan

Lin

Guo

et al. 2018

Sci Rep

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To elucidate the mechanisms of molecular regulations underlying primary dysmenorrhea (PD), we used our previously published mRNA expression profile of uterus from PD syndrome rats to construct protein-protein interactions (PPI) network via STRING Interactome. Consequently, 34 subnetworks, including a “continent” (Subnetwork 1) and 33 “islands” (Subnetwork 2–34) were generated. The nodes, with relative expression ratios, were visualized in the PPI networks and their connections were identified. Through path and module exploring in the network, the bridges were found from pathways of cellular response to calcium ion, SMAD protein signal transduction, regulation of transcription from RNA polymerase II promoter in response to stress and muscle stretch that were significantly enriched by the up-regulated mRNAs, to the cascades of cAMP metabolic processes and positive regulation of cyclase activities by the down-regulated ones. This link is mainly dependent on Fos/Jun - Vip connection. Our data, for the first time, report the PPI network analysis of differentially expressed mRNAs in the uterus of PD syndrome rats, to give insight into screening drugs and find new therapeutic strategies to relieve PD.

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Section: Discussionmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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The PPI network analysis of mRNA expression profile of uterus from primary dysmenorrheal rats

Fan

Lin

Guo

et al. 2018

Sci Rep

Self Cite

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“…Recent evidences have revealed that microRNAs may contribute to warfarin dose requirement and be associated with risk factors for arterial and venous thrombosis 10. Recent studies have indicated that targeting some microRNAs (miRNAs) may be useful to improve outcome of patients with venous thromboembolism 11.…”

Section: Introductionmentioning

confidence: 99%

Association between polymorphisms in microRNA seed region and warfarin stable dose

Hosseindokht

Zare

Salehi

et al. 2020

Postgraduate Medical Journal

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BackgroundThe optimal dose of anticoagulant warfarin varies among patients to achieve the target international normalised ratio. Although genetic variations related to warfarin pharmacokinetics and vitamin K cycle are important factors associated with warfarin dose requirements, these variations do not completely explain the large interindividual variability observed in the most populations, suggesting that additional factors may contribute to this variability. microRNAs have recently been introduced as regulators of drug function genes, and therefore, may be involved in drug responses. In this study, we aimed to evaluate the possible association between variants in the seed region of microRNAs, which target the genes involved in the action of warfarin and warfarin dose requirement.Methods526 samples were collected from Iranian patients. Four selected polymorphisms in the seed region of microRNAs (rs2910164, rs66683138, rs12416605 and rs35770269 in miR-146a, miR-3622a, miR-938 and miR-449c, respectively) were genotyped by PCR-restriction fragment length polymorphism method.Resultsrs2910164 C/G in the seed region of miR-146a was associated with warfarin dose requirement (p<0.001); the patients with GG genotype had the higher mean dose of warfarin (40.6 mg/week, compared with 33.9 and 31.8 mg/week for GC and CC genotypes, respectively). The association of other polymorphisms with warfarin dose requirement was not statistically significant.Conclusionrs2910164 C/G in the seed region of miR-146a is associated with warfarin maintenance dose, likely by disrupting interaction between miR-146a and ATP-binding cassette subfamily B member 1 gene, ABCB1. Therefore, this polymorphism may possibly be a potential factor for assessment of warfarin dose requirements.

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Association study of miR-146a, miR-149, miR-196a2, and miR-499 polymorphisms with venous thromboembolism in a Korean population

Kim

et al. 2019

J Thromb Thrombolysis

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miR-21-5p is associated with the regulation of estradiol benzoate and oxytocin induced primary dysmenorrhea in rat uterus: a bioinformatic study

Cited by 3 publications

References 40 publications

The PPI network analysis of mRNA expression profile of uterus from primary dysmenorrheal rats

The PPI network analysis of mRNA expression profile of uterus from primary dysmenorrheal rats

Association between polymorphisms in microRNA seed region and warfarin stable dose

Association study of miR-146a, miR-149, miR-196a2, and miR-499 polymorphisms with venous thromboembolism in a Korean population

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