miR-218 suppresses gastric cancer cell cycle progression through the CDK6/Cyclin D1/E2F1 axis in a feedback loop

Deng, Min; Zeng, Chao; Lu, Xihong; He, Xiu‐Sheng; Zhang, Ruixin; Qiu, Qinwei; Zheng, Guopei; Jia, Xiaoting; Líu, Hao; He, Zhiwei

doi:10.1016/j.canlet.2017.06.006

Cited by 88 publications

(72 citation statements)

References 40 publications

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“…Previous studies have suggested that several miRNAs were abnormal expression in the progress of periodontal disease and regulated multifarious genes such as RUNX2, osteocalcin (OC), and EGFR (Motedayyen, Ghotloo, Saffari, Sattari, & Amid, ; Xie et al, ; Zhou et al, ). MiR‐218, a tumour suppressor, is significantly downregulated in various cancers (Deng et al, ; Shi et al, ) and inflammatory disorders (Xu et al, ). For example, the decrease of serum miR‐218 levels was observed in the inflammation induced by cigarette smoking, and miR‐218 overexpression would repress TNFR1‐mediated activation of NF‐κB and resulted in decreasing levels of serum IL‐6 and IL‐8 (Xu et al, ).…”

Section: Discussionmentioning

confidence: 99%

Section: Mir-218 Decreased Bone Destruction and Inflammation In Ligmentioning

confidence: 99%

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Expression of Concern: MiRNA‐218 regulates osteoclast differentiation and inflammation response in periodontitis rats through Mmp9

Guo

Zeng

Miao

et al. 2019

Cellular Microbiology

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Periodontitis is a multiple infection and inflammatory disease featured by connective tissue homeostasis loss, periodontal inflammation, and alveolar bone resorption. MicroRNAs (miRNAs) are involved in the mediation of a large scale of pathological processes. Here, we show that miRNA‐218 provides protective effect on periodontitis via regulation of matrix metalloproteinase‐9 (Mmp9). This pathway is aberrant in periodontium from rats with periodontitis and human periodontal ligament progenitor cells stimulated by lipopolysaccharide, with downregulation of miR‐218 and higher levels of Mmp9 compared with periodontium from healthy rats and cells without stimulation. Overexpression of miR‐218 can suppress the degradation of Collagen Types I and IV and dentin sialoprotein (DSP), attenuate osteoclast formation, and inhibit the secretion of proinflammatory cytokines. On the other hand, overexpression of Mmp9 promotes the degradation of Collagen Types I and IV and DSP as well as RANKL‐induced osteoclast formation and elevates inflammatory factors levels. Furthermore, the inhibitory effect of miR‐218 was prevented by rescuing the Mmp9 expression. In addition, we also have showed that miR‐218 was able to attenuate bone resorption and inflammation in a periodontitis rat model. Collectively, our findings therefore suggest that miR‐218 acts as a protective role in periodontitis through the regulation of Mmp9.

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Section: Discussionmentioning

confidence: 99%

Section: Mir-218 Decreased Bone Destruction and Inflammation In Ligmentioning

confidence: 99%

Expression of Concern: MiRNA‐218 regulates osteoclast differentiation and inflammation response in periodontitis rats through Mmp9

Guo

Zeng

Miao

et al. 2019

Cellular Microbiology

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“…Several lines of literature have reported that miR-218–5p was downexpressed in multiple human cancers, including hepatocellular carcinoma [49], pancreatic ductal adenocarcinoma [50], gastric cancer [51], lung cancer [52], and gallbladder cancer [53]. However, miR-218–5p has been reported to function as an oncogenic miRNA in glioma [54], suggesting that the pro- and anti-cancer roles of miR-218–5p are tumor-type dependent.…”

Section: Discussionmentioning

confidence: 99%

Decreased miR-218–5p Levels as a Serum Biomarker in Bone Metastasis of Prostate Cancer

Peng¹,

Chen

Wang

et al. 2019

Oncol Res Treat

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Background: miR-218–5p is an extensively studied microRNA (miRNA) in prostate cancer (PCa). However, the clinical significance and biological role of miR-218–5p in bone metastasis of PCa remain unclear. Materials and Methods: miR-218–5p expression was evaluated in 38 bone metastatic and 115 non-bone metastatic PCa tissues and serum samples. Clinical correlation of miR-218–5p expression with clinicopathological characteristics was analyzed. The biological roles of miR-218–5p in bone metastasis of PCa were investigated in vitro by invasion and migration assays. Bioinformatics analysis, real-time polymerase chain reaction, western blot, and luciferase reporter assay were applied to discern and examine the relationship between miR-218–5p and its potential targets. Results: miR-218–5p expression was reduced in bone metastatic PCa tissue and serum samples, which positively correlated with poor clinicopathological characteristics and bone metastasis-free survival in PCa patients. Upregulating miR-218–5p repressed PCa cell invasion and migration. Furthermore, miR-218–5p inhibited NF-κB signaling via simultaneously targeting TRAF1, TRAF2, and TRAF5, which suppressed the invasion and migration abilities of PCa cells. ROC curve analysis of miR-218–5p in the serum of PCa patients exhibited an area under the curve of 0.86 (95% confidence interval 0.80–0.92, p < 0.001). Conclusion: Our findings indicate that miR-218–5p might represent a novel serum biomarker for bone metastasis of PCa.

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“…The development of cancer mainly depends on the balance of pro-apoptotic proteins, such as Bax, and anti-apoptotic proteins, such as Bcl-2 [31].The p53 protein is a regulatory factor of many processes necessary for the proper functioning of cells. It plays a critical role as a tumour suppressor, inducing cell cycle arrest and apoptosis [32].CyclinD1,as a major member of the cyclins, plays an important role in cell cycle progression [33].…”

Section: Discussionmentioning

confidence: 99%

Bushenshugan Formula Attenuates the Development of Lung Cancer by Inhibiting Epithelial-Mesenchymal Transition

Fan

Xue

Dou

et al. 2018

Cell Physiol Biochem

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Background/Aims: BushenShugan Formula (BSF) is a traditional Chinese medicine that has therapeutic effects on middle- and late-stage lung adenocarcinoma in clinical application. It was reported that Bushen Chinese medicine suppressed the onset of pre-metastatic niches in a murine model of spontaneous lung metastasis. However, the mechanisms of BSF on human lung adenocarcinoma remain unknown. Methods: Cell proliferation was determined by CCK8 and colony formation. Cell apoptosis and cell cycle were detected by flow cytometry. Cancer stem cells properties were examined by spheroid body formation. The migration and invasion abilities were analyzed by wound healing assay and transwell invasion assay. The mRNA expressions were determined by qRT-PCR. Western blotting analysis showed the protein levels. Results: BSF was shown to inhibit the proliferation of A549 cells in time- and concentration-dependent manners. Colony formation assays also indicated the antiproliferative effect of BSF against A549 cells. Cellular mechanistic studies demonstrated that BSF arrested the cell cycle in G2/M phase and induced apoptosis. Importantly, BSF could inhibit the epithelial-mesenchymal transition(EMT) of A549 cells through PI3K/AKT/NF-κB pathway. Conclusions: BSF effectively inhibited tumour growth, suggesting that it is a promising anticancer treatment for further clinical development.

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miR-218 suppresses gastric cancer cell cycle progression through the CDK6/Cyclin D1/E2F1 axis in a feedback loop

Cited by 88 publications

References 40 publications

Expression of Concern: MiRNA‐218 regulates osteoclast differentiation and inflammation response in periodontitis rats through Mmp9

Expression of Concern: MiRNA‐218 regulates osteoclast differentiation and inflammation response in periodontitis rats through Mmp9

Decreased miR-218–5p Levels as a Serum Biomarker in Bone Metastasis of Prostate Cancer

Bushenshugan Formula Attenuates the Development of Lung Cancer by Inhibiting Epithelial-Mesenchymal Transition

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