MiR-223 Exclusively Impairs In Vitro Tumor Growth through IGF1R Modulation in Rhabdomyosarcoma of Adolescents and Young Adults

Abstract

Adolescents and young adults (AYA) with rhabdomyosarcoma (RMS) form a subgroup of patients whose optimal clinical management and best possible access to care remain a challenge and whose survival rates lag behind that of children diagnosed with histologically similar tumors. A better understanding of tumor biology that differentiates children (PEDS-) from AYA-RMS could provide critical information and drive new initiatives to improve their final outcome. We investigated the functional role of miRNAs implicated in AYA-RMS development, as they have the potential to lead to discovery of new targets pathways for a more tailored treatment in these age groups of young RMS patients. MiR-223 and miR-486 were observed de-regulated in nine RMS tissues compared to their normal counterparts, yet only miR-223 replacement impaired proliferation and aggressiveness of AYA-RMS cell lines, while inducing apoptosis and determining cell cycle arrest. Interestingly, IGF1R resulted in the direct target of miR-223 in AYA-RMS cells, as demonstrated by IGF1R silencing. Our results highlight an exclusive functional role of miR-223 in AYA-RMS development and aggressiveness.

Hepatocellular Carcinoma Prediction and early Diagnosis of Hepatitis B and C viral infection using miR-122 and miR-223 in a sample of Iraqi patients.

“…This suggests that the downregulation of miR-122 and miR-223 could be used as reliable biomarkers to differentiate HBV and HCV infections from normal healthy samples. The results of this study are consistent with those of other studies 20,17,8 . However, found that HCC patients exhibited significantly higher levels of miR-223 than healthy individuals in their study.…”

“…It is evident that miR-223 plays a significant role in the progression and development of cancers. There is evidence that miR-223 regulates multiple aspects of the immune response, and that abnormal miR-223 expression is associated with multiple infectious diseases, including viral hepatitis 17,18 . In addition to providing an overview of the miR-122 and miR-223 levels in samples from patients with HBV, HCV, and healthy controls, the data was also presented as a heat map.…”

Novel Therapeutic Targets in Cancers

“…Two examples of the first kind are described in this Special Issue. Casanova et al [15] studied miR-223 in rhabdomyosarcoma (RMS), a type of muscle cancer that affects adolescents and young adults (AYA) more severely than younger children. They found that miR-223 expression was lower in RMS than normal tissues.…”