Mir-23a and mir-125b regulate neural stem/progenitor cell proliferation by targeting Musashi1

Gioia, Ubaldo; Caramanica, Pasquale; Toselli, Camilla; Cinquino, Antonella; Marchioni, Marcella; Laneve, Pietro; Biagioni, Stefano; Bozzoni, Irene; Cacci, Emanuele; Caffarelli, Elisa

doi:10.4161/rna.35508

Cited by 29 publications

(25 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The let‐7c miRNA was described as an essential regulator involved in the progress of embryo development (Lin et al, ). Similarly, miR‐23a regulates cellular maintenance and differentiation in mouse embryonic stem cells (Gioia et al, ). Among the already mentioned miRNAs, miR‐96, let‐7c, and miR‐23a were identified in the human placenta of in vivo and successful pregnancies (Li et al, ).…”

Section: Discussionmentioning

confidence: 99%

Oxygen tension modulates extracellular vesicles and its miRNA contents in bovine embryo culture medium

Andrade

Bomfim

Collado

et al. 2019

Molecular Reproduction Devel

View full text Add to dashboard Cite

The biotechnology for in vitro embryo production is becoming increasingly popular, being applied to humans and domestic animals. Embryo development can be achieved with either 20% or 5% oxygen tension. The extracellular vesicles (EVs) are secreted by different cell types and carry bioactive materials. Our objective was to determine the secretion pattern and micro RNA (miRNA) contents of EVs released in the bovine embryo culture environment—embryo and cumulus cell monolayer—on Days 3 and 7 of in vitro culture under two different oxygen tensions: High (20%) and low (5%). The EVs were isolated from the medium and analyzed to determine size, concentration, and miRNA levels. EVs concentration in low oxygen tension increased on Day 3 and decreased on Day 7. Additionally, altered EV miRNAs derived from the embryo‐cumulus culture medium were predicted to regulate survival and proliferation‐related pathways on Days 3 and 7. Moreover, miR‐210 levels decreased in EVs isolated from the culture medium under high oxygen tension suggesting that this miRNA can be used as a marker for normoxia since it is associated with low oxygen tension. In summary, this study provides knowledge of the oxygen tension effects on EVs release and content, and potentially, on cell‐to‐cell communication during in vitro bovine embryo production.

show abstract

Section: Discussionmentioning

confidence: 99%

Oxygen tension modulates extracellular vesicles and its miRNA contents in bovine embryo culture medium

Andrade

Bomfim

Collado

et al. 2019

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…In order to identify molecular cargoes in astrocyte-derived sEVs able to promote morphological changes in neurons, we took advantage of a comprehensive study by Jovičić et al identifying miRNAs in total homogenates from cultured astrocytes [33]. To generate a robust short-list of candidates, we selected the most abundant miRNAs (cycle threshold < 25), which have been confirmed to be present in astrocytes by at least one other independent study [13,[34][35][36][37][38][39][40][41][42]. Then, the identified candidates, namely miR-26a5p, miR-23a-5p, miR-223a-5p, miR-19a-5p, miR-32a-5p, miR-146a-5p, miR-181-5p, and miR-29a-5p, were categorized using a selection algorithm according to the function of their predicted targets (see Methods; Figure 3A).…”

Section: Astrocyte-derived Sevs Carry Mir-26-5p Which Targets Gene Ementioning

confidence: 99%

Astrocyte-Derived Small Extracellular Vesicles Regulate Dendritic Complexity through miR-26a-5p Activity

Luarte

Henzi

Fernández

et al. 2020

Cells

View full text Add to dashboard Cite

In the last few decades, it has been established that astrocytes play key roles in the regulation of neuronal morphology. However, the contribution of astrocyte-derived small extracellular vesicles (sEVs) to morphological differentiation of neurons has only recently been addressed. Here, we showed that cultured astrocytes expressing a GFP-tagged version of the stress-regulated astrocytic enzyme Aldolase C (Aldo C-GFP) release small extracellular vesicles (sEVs) that are transferred into cultured hippocampal neurons. Surprisingly, Aldo C-GFP-containing sEVs (Aldo C-GFP sEVs) displayed an exacerbated capacity to reduce the dendritic complexity in developing hippocampal neurons compared to sEVs derived from control (i.e., GFP-expressing) astrocytes. Using bioinformatics and biochemical tools, we found that the total content of overexpressed Aldo C-GFP correlates with an increased content of endogenous miRNA-26a-5p in both total astrocyte homogenates and sEVs. Notably, neurons magnetofected with a nucleotide sequence that mimics endogenous miRNA-26a-5p (mimic 26a-5p) not only decreased the levels of neuronal proteins associated to morphogenesis regulation, but also reproduced morphological changes induced by Aldo-C-GFP sEVs. Furthermore, neurons magnetofected with a sequence targeting miRNA-26a-5p (antago 26a-5p) were largely resistant to Aldo C-GFP sEVs. Our results support a novel and complex level of astrocyte-to-neuron communication mediated by astrocyte-derived sEVs and the activity of their miRNA content.

show abstract

“…It has been estimated that more than one-third of all protein-coding genes seem to be miRNA targets in humans by conserved seed pairing [12]. A growing body of evidence has revealed that miRNAs are involved in diverse physiological processes, such as development [13], cell proliferation and differentiation [14, 15], apoptosis [16], and a variety of pathological conditions [17, 18]. Similarly, recent studies have also focused on the involvement of miRNAs in virus-host interaction networks.…”

Section: Introductionmentioning

confidence: 99%

Systematic Identification and Bioinformatic Analysis of MicroRNAs in Response to Infections of Coxsackievirus A16 and Enterovirus 71

Zhu

Fan

et al. 2016

BioMed Research International

View full text Add to dashboard Cite

Hand, foot, and mouth disease (HFMD), mainly caused by coxsackievirus A16 (CVA16) and enterovirus 71 (EV71) infections, remains a serious public health issue with thousands of newly diagnostic cases each year since 2008 in China. The mechanisms underlying viral infection, however, are elusive to date. In the present study, we systematically investigated the host cellular microRNA (miRNA) expression patterns in response to CVA16 and EV71 infections. Through microarray examination, 27 miRNAs (15 upregulated and 12 downregulated) were found to be coassociated with the replication process of two viruses, while the expression levels of 15 and 5 miRNAs were significantly changed in CVA16- and EV71-infected cells, respectively. A great number of target genes of 27 common differentially expressed miRNAs were predicted by combined use of two computational target prediction algorithms, TargetScan and MiRanda. Comprehensive bioinformatic analysis of target genes in GO categories and KEGG pathways indicated the involvement of diverse biological functions and signaling pathways during viral infection. These results provide an overview of the roles of miRNAs in virus-host interaction, which will contribute to further understanding of HFMD pathological mechanisms.

show abstract

Mir-23a and mir-125b regulate neural stem/progenitor cell proliferation by targeting Musashi1

Cited by 29 publications

References 31 publications

Oxygen tension modulates extracellular vesicles and its miRNA contents in bovine embryo culture medium

Oxygen tension modulates extracellular vesicles and its miRNA contents in bovine embryo culture medium

Astrocyte-Derived Small Extracellular Vesicles Regulate Dendritic Complexity through miR-26a-5p Activity

Systematic Identification and Bioinformatic Analysis of MicroRNAs in Response to Infections of Coxsackievirus A16 and Enterovirus 71

Contact Info

Product

Resources

About