miR-23b-3p and miR-130a-5p affect cell growth, migration and invasion by targeting CB1R via the Wnt/β-catenin signaling pathway in gastric carcinoma

Abstract

BackgroundGastric cancer (GC) is the most common malignancy and third leading cause of cancer mortality worldwide. The identification of a sensitive biomarker as well as effective therapeutic targets for the treatment of GC is of critical importance. microRNAs play significant roles in the development of cancer and may serve as promising therapeutic targets.MethodsThe mRNA and protein expression of CB1R were studied both in GC cells and tissues. GC cell lines with specific gene overexpression and knockdown vectors were constructed. CCK-8 assay, matrigel invasion and colony formation assays were performed to evaluate the proliferation and invasion abilities. The binding and regulatory effects of miR-23b-3 and miR-130a-5p on CB1R mRNA were investigated using a luciferase reporter assay. Western blot analysis was performed to explore the potential interaction proteins of CB1R.ResultsIn the present study, it was demonstrated that the cannabinoid receptor 1 (CB1R) was overexpressed, and miR-23b-3p and miR-130a-5p were downregulated, in GC cells. In addition, the results revealed that these effects are associated with malignant biological behaviors exhibited by GC cells. Furthermore, miR-23b-3p and miR-130a-5p may regulate CB1R expression via the Wnt/β-catenin signaling pathway.ConclusionOur results suggested dysregulation of CB1R expression is closely related to the malignant biological behavior of gastric cancer cells. miRNA/CB1R-based therapy may represent a promising therapeutic strategy for the clinical treatment of GC patients.

Analysis of Exosome-derived MicroRNAs as Early Biomarkers of Lipopolysaccharide-induced Acute Kidney Injury in Rats

“…We also observed upregulation of miR-23b-3p, and similar to miR-181a-5p, this miRNA is also associated with several types of neoplasms [40][41][42][43][44]. Interestingly, the presence of miR-23b-3p in human urinary exosomes has been reported, and its expression was increased in urinary exosomes of patients with nephrotic syndrome [45].…”

Cannabinoids Promote Progression of HPV-Positive Head and Neck Squamous Cell Carcinoma via p38 MAPK Activation

“…immunoreactivity was associated with disease severity and outcome in esophageal cancer (20), prostate cancer (44), pancreatic cancer (45), ovarian cancer (46) and others. Furthermore, CNR1 knockout mice developed smaller and fewer tumors in a diethylnitrosamine induced hepatocellular carcinoma model (15), and overexpression of CNR1 in esophageal squamous cell carcinoma and gastric cancer revealed that CNR1 activation could promote cell proliferation and invasion (20,47). In this study, although TCGA data showed that the expression of CNR1 mRNA was downregulated in HNSCC compared with normal samples, CNR1 mRNA was highly expressed in HPV positive HNSCC compared with HPV negative HNSCC (Fig.…”

MiR-130a in the adipogenesis of human SGBS preadipocytes and its susceptibility to androgen regulation

“…Additionally, miR-130a was demonstrated to be upregulated by hypoxia in gastric cells, also targeting RUNX3 and therefore increasing -in combination with miR-495cell proliferation while lowering apoptosis [34]. These interactions may also be exerted via the Wnt/β-Catenin signalling pathway, as this axis was demonstrated to be active in gastric cancer cells [35]. Together, these data point towards a regulative role of miR-130a during the early phase of several differentiation processes.…”