MiR-24-3p Conservatively Regulates Muscle Cell Proliferation and Apoptosis by Targeting Common Gene CAMK2B in Rat and Cattle

Abstract

Skeletal muscle plays an important role in the growth and development of meat animals. MicroRNAs (miRNAs) can participate in the regulation of muscle development-related functions; however, there have been few reports on whether there are related miRNAs that conservatively regulate muscle development among different species. In this study, the miRNA transcriptome sequencing data of the muscle tissue of cattle, rat, goat, and pig showed that miR-24-3p may conservatively regulate muscle development in these species. Furthermore, mmu-miR-24-3p can positively regulate C2C12 cell proliferation and apoptosis by regulating key proliferation and apoptosis genes in muscle development, which was verified by CCK-8 and RT-qPCR. Bta-miR-24-3p can also positively regulate the proliferation and apoptosis of bovine muscle primary cells by regulating key proliferation and apoptosis genes in the process of muscle development, as verified by CCK-8 and RT-qPCR. The target genes of miR-24-3p in cattle, rat, goat, and pig, which include a large proportion of target genes shared among the four species, are enriched in multiple cell functions and signal pathways that are closely related to muscle development, as revealed by GO and KEGG enrichment analysis. A double luciferase test showed that the shared target genes WNT4, CAMK2B, and TCF7 were targeted by mmu-miR-24-3p in rat and bta-miR-24-3p in cattle. These three shared target genes WNT4, CAMK2B, and TCF7 are involved in the Wnt signaling pathway, which showed that miR-24-3p plays an important role in rat and cattle. The shared target gene (CAMK2B) in rat and cattle increased significantly after the inhibition of miR-24-3p by RT-qPCR. The findings of this study contribute to a better understanding of the role of miR-24-3p in the regulation of muscle development.

Comparative Genomics Identifies the Evolutionarily Conserved Gene TPM3 as a Target of eca-miR-1 Involved in the Skeletal Muscle Development of Donkeys

“…In this study, we used mRNA and miRNA sequencing to profile the skeletal muscle transcriptome and, thus, identify genes and miRNAs that were differentially expressed between donkeys with different feed efficiencies, including the transcriptome data of S1 (2-month-old Dezhou donkey) and S2 (24-month-old Dezhou donkey). The comparative transcriptome analysis results showed that a total of 45 miRNAs and 17 genes were differentially expressed, indicating that these miRNAs and genes may be related to muscle development in donkeys, which is consistent with previous studies [10][11][12][13][14]. Additionally, we found that eca-miR-1 was differentially expressed in S1 and S2; moreover, we also noticed that TPM3 genes were differentially expressed in S1 and S2 transcriptomes (Figure 4).…”

“…The domestication of donkeys and horses has played an important role in human life, with these animals being valued for their draft ability, primarily based on their muscular strength [9,10]. Skeletal muscle plays an indispensable role in their bodies, as it is a dynamic tissue [37,38].…”

“…Previous studies have used collinearity analysis to identify the role of evolutionarily conserved genes [7,8]. Skeletal muscle in the Equus genus plays an important role as a dynamic tissue in the body, and exercise ability is very important for donkeys and horses, which drives their important economic value [9,10]. An analysis of the fatty acid composition of donkey intramuscular fat showed that the content of polyunsaturated fatty acids (PUFAs) in both longissimus dorsi and biceps femoris muscles at 25.16 g/100 g and 24.97 g/100 g total fatty acids, respectively [9].…”

Exploring Multi-Tissue Alternative Splicing and Skeletal Muscle Metabolism Regulation in Obese- and Lean-Type Pigs

“…The results showed that the most significant enrichment pathway was the glucagon signaling pathway. Through our investigation, we focused on three genes associated with skeletal muscle development in this pathway: PYGM [63], MAPK11 [64] and CAMK2B [65]. As demonstrated by our results (Figure 6), the PSI values for these three genes showed significant differences in skeletal muscle of two pig breeds.…”

miR-24-3p Dominates the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes by Blocking ANXA6 Expression

“…For the reason that miR-24-3p is evolutionarily conserved, its regulatory effects are theoretically similar in different species. In recent studies, miR-24-3p was proved to promote proliferation and inhibit apoptosis of C2C12 cells by targeting CAMK2B [25]. Moreover, miR-24-3p promoted cell migration and proliferation in lung cancer by targeting SOX7 [26].…”