MiR-25-3p regulates the differentiation of intramuscular preadipocytes in goat via targeting &amp;lt;i&amp;gt;KLF4&amp;lt;/i&amp;gt;

Du, Yuguang; Zhao, Yiqing; Wang, Yong; Meng, Qingyong; Zhu, Jiangjiang; Lin, Yaqiu

doi:10.5194/aab-64-17-2021

Cited by 6 publications

(4 citation statements)

References 40 publications

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“…Goat intramuscular preadipocytes (GIMPA) were isolated from the longissimus dorsi muscle (Sichuan jianyang dageda animal husbandry CO., LTD, Sichuan, China) of 7-day-old Jianzhou Da-er goats. The isolation and culture of cells was consistent with the previous study [ 25 ]. DMEM-F12 (Gibco, Carlsbad, CA, USA) containing 10% FBS (fetal bovine serum, Gibco, USA), 1‰ Penicillin-Streptomycin (Gibco, Carlsbad, CA, USA) and 50 μmol·L −1 oleic acid (Sigma, St Louis, MO, USA) was used to induce adipogenic differentiation of GIMPA to goat intramuscular adipocytes (GIMA).…”

Section: Methodssupporting

confidence: 83%

“…ncRNAs, including microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), have also been shown to be important regulators of adipocyte differentiation in recent years [ 21 , 22 , 23 ]. For instance, miR-340-5p inhibits ovine adipocyte differentiation by targeting ATF7 [ 24 ] and miR-25-3p regulates the differentiation of intramuscular preadipocytes in goats via targeting KLF4 [ 25 ]. He et al (2022) previously constructed an lncRNA-miRNA-mRNA regulatory network during goat intramuscular and subcutaneous adipocyte differentiation by RNA-seq, and identified several lncRNAs that may regulate adipocyte differentiation [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Chi-Circ_0006511 Positively Regulates the Differentiation of Goat Intramuscular Adipocytes via Novel-miR-87/CD36 Axis

Zhang

Wang

et al. 2022

IJMS

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Goats are an important livestock and goat meat is essential to local people. The intramuscular fat (IMF) content has a great influence on the quality of goat meat. The intramuscular preadipocytes differentiation is closely related to the IMF deposition; however, its potential regulatory mechanisms remain unclear. CircRNAs were revealed to be involved in multiple biological progressions. In this study, we took primary goat intramuscular preadipocyte (GIMPA) as the study model to verify the function and mechanism of chi-circ_0006511, which was abundant and up-regulated in mature adipocytes (GIMA). The results showed that the expression level of chi-circ_0006511 gradually increased in the early stage of GIMPA differentiation, and chi-circ_0006511 was confirmed to promote GIMPA lipid droplets aggregation and up-regulate the adipogenic differentiation determinants, further promoting GIMPA differentiation. Mechanistically, chi-circ_0006511 exerts its function by sponging novel-miR-87, thereby regulating the expression of CD36. The results from this study provided novel significant information to better understand the molecular regulatory mechanism of intramuscular preadipocytes differentiation, thereby providing a new reference for the intramuscular fat adipogenesis in goats.

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Section: Methodssupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Chi-Circ_0006511 Positively Regulates the Differentiation of Goat Intramuscular Adipocytes via Novel-miR-87/CD36 Axis

Zhang

Wang

et al. 2022

IJMS

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“…Currently, thousands of miRNA gene codes have been recognized [ 37 ]. The interaction involves the formation of a double-stranded assembly between the miRNA seed and the mRNA “target” [ 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

“…Currently, thousands of miRNA gene codes have been recognized [37]. The interaction involves the formation of a double-stranded assembly between the miRNA seed and the mRNA "target" [38,39]. Kim et al 2010 [40] have described the relationship between miRNAs and the regulation of early adipocyte differentiation.…”

Section: Introductionmentioning

confidence: 99%

The miRNA Contribution in Adipocyte Maturation

Giammona,

Di Franco,

Lo Dico

et al. 2024

ncRNA

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Mesenchymal stem cells, due to their multipotent ability, are considered one of the best candidates to be used in regenerative medicine. To date, the most used source is represented by the bone marrow, despite the limited number of cells and the painful/invasive procedure for collection. Therefore, the scientific community has investigated many alternative sources for the collection of mesenchymal stem cells, with the adipose tissue representing the best option, given the abundance of mesenchymal stem cells and the easy access. Although adipose mesenchymal stem cells have recently been investigated for their multipotency, the molecular mechanisms underlying their adipogenic potential are still unclear. In this scenario, this communication is aimed at defining the role of miRNAs in adipogenic potential of adipose-derived mesenchymal stem cells via real-time PCR. Even if preliminary, our data show that cell culture conditions affect the expression of specific miRNA involved in the adipogenic potential of mesenchymal stem cells. The in vitro/in vivo validation of these results could pave the way for novel therapeutic strategies in the field of regenerative medicine. In conclusion, our research highlights how specific cell culture conditions can modulate the adipogenic potential of adipose mesenchymal stem cells through the regulation of specific miRNAs.

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Circular RNA_015343 sponges microRNA‐25 to regulate viability, proliferation, and milk fat synthesis of ovine mammary epithelial cells via INSIG1

Wu,

Wang,

Hao

et al. 2024

Journal Cellular Physiology

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In our previous study, circ_015343 was found to inhibit the viability and proliferation of ovine mammary epithelial cells (OMECs) and the expression levels of milk fat synthesis marker genes, but the regulatory mechanism underlying the processes is still unclear. Accordingly in this study, the target relationships between circ_015343 with miR‐25 and between miR‐25 with insulin induced gene 1 (INSIG1) were verified, and the functions of miR‐25 and INSIG1 were investigated in OMECs. The dual‐luciferase reporter assay revealed that miR‐25 mimic remarkably decreased the luciferase activity of circ_015343 in HEK293T cells cotransfected with a wild‐type vector, while it did not change the activity of circ_015343 in HEK293T cells cotransfected with a mutant vector. These suggest that cic_015343 can adsorb and bind miR‐25. The miR‐25 increased the viability and proliferation of OMECs, and the content of triglycerides in OMECs. In addition, INSIG1 was found to be a target gene of miR‐25 using a dual‐luciferase reporter assay. Overexpression of INSIG1 decreased the viability, proliferation, and level of triglycerides of OMECs. In contrast, the inhibition of INSIG1 in expression had the opposite effect on activities and triglycerides of OMECs with overexpressed INSIG1. A rescue experiment revealed that circ_015343 alleviated the inhibitory effect of miR‐25 on the mRNA and protein abundance of INSIG1. These results indicate that circ_015343 sponges miR‐25 to inhibit the activities and content of triglycerides of OMECs by upregulating the expression of INSIG1 in OMECs. This study provided new insights for understanding the genetic molecular mechanism of lactation traits in sheep.

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MiR-25-3p regulates the differentiation of intramuscular preadipocytes in goat via targeting <i>KLF4</i>

Cited by 6 publications

References 40 publications

Chi-Circ_0006511 Positively Regulates the Differentiation of Goat Intramuscular Adipocytes via Novel-miR-87/CD36 Axis

Chi-Circ_0006511 Positively Regulates the Differentiation of Goat Intramuscular Adipocytes via Novel-miR-87/CD36 Axis

The miRNA Contribution in Adipocyte Maturation

Circular RNA_015343 sponges microRNA‐25 to regulate viability, proliferation, and milk fat synthesis of ovine mammary epithelial cells via INSIG1

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