miR‐26a inhibits proliferation and promotes apoptosis in porcine immature Sertoli cells by targeting the <i>PAK2</i> gene

Ran, Maoliang; Wang, Bo; Cao, Rong; Li, Zhi; Peng, Fuzhi; Luo, Hongyu; Hu, Gao; Chen, Bin

doi:10.1111/rda.13254

Cited by 22 publications

(20 citation statements)

References 45 publications

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“…h The quantification of PLCβ1 protein. *P < 0.05, **P < 0.01, ***P < 0.001, PLCβ1, phospholipase C beta 1; UTR, Untranslated Region; ST, swine testis; NC, negative control; N.S., nonsignificant processes of animal testis development and spermatogenesis via their regulations of cell proliferation, apoptosis, and differentiation [29][30][31][32].…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars

et al. 2020

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Background MicroRNAs (miRNAs) are small non-coding RNAs playing vital roles in regulating posttranscriptional gene expression. Elucidating the expression regulation of miRNAs underlying pig testis development will contribute to a better understanding of boar fertility and spermatogenesis. Results In this study, miRNA expression profile was investigated in testes of Duroc and Meishan boars at 20, 75, and 270 days of age by high-throughput sequencing. Forty-five differentially expressed miRNAs were identified from testes of Duroc and Meishan boars before and after puberty. Integrated analysis of miRNA and mRNA profiles predicted many miRNA-mRNA pairs. Gene ontology and biological pathway analyses revealed that predicted target genes of ssc-mir-423-5p, ssc-mir-34c, ssc-mir-107, ssc-mir-196b-5p, ssc-mir-92a, ssc-mir-320, ssc-mir-10a-5p, and ssc-mir-181b were involved in sexual reproduction, male gamete generation, and spermatogenesis, and GnRH, Wnt, and MAPK signaling pathway. Four significantly differentially expressed miRNAs and their predicted target genes were validated by quantitative real-time polymerase chain reaction, and phospholipase C beta 1 (PLCβ1) gene was verified to be a target of ssc-mir-423-5p. Conclusions This study provides an insight into the functional roles of miRNAs in testis development and spermatogenesis and offers useful resources for understanding differences in sexual function development caused by the change in miRNAs expression between Duroc and Meishan boars.

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Section: Discussionmentioning

confidence: 99%

Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars

et al. 2020

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“…These miRNAs may serve potential roles in regulating testis development, and their speci c expressions may induce the differences in sexual maturity and spermatogenesis. A growing number of reports have revealed that miRNAs play important roles in the complex processes of animal testis development and spermatogenesis via their regulations of cell proliferation, apoptosis, and differentiation [29][30][31][32].…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars

Ding

Liu

Zhou

et al. 2020

Preprint

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Background: MicroRNAs (miRNAs) are small non-coding RNAs playing vital roles in regulating posttranscriptional gene expression. Elucidating the expression regulation of miRNAs underlying pig testis development will contribute to a better understanding of boar fertility and spermatogenesis. Results: In this study, miRNA expression profile was investigated in testes of Duroc and Meishan boars at 20, 75, and 270 days of age by high-throughput sequencing. Forty-five differentially expressed miRNAs were identified from testes of Duroc and Meishan boars before and after puberty. Integrated analysis of miRNA and mRNA profiles predicted many miRNA-mRNA pairs. Gene ontology and biological pathway analyses revealed that predicted target genes of ssc-mir-423-5p, ssc-mir-34c, ssc-mir-107, ssc-165 mir-196b-5p, ssc-mir-92a, ssc-mir-320, ssc-mir-10a-5p, and ssc-mir-181b were involved in sexual reproduction, male gamete generation, and spermatogenesis, and GnRH, Wnt, and MAPK signaling pathway. Four significantly differentially expressed miRNAs and their predicted target genes were validated by quantitative real-time polymerase chain reaction, and phospholipase C beta 1 ( PLCβ1) gene was verified to be a target of ssc-mir-423-5p . Conclusions: This study provides an insight into the functional roles of miRNAs in testis development and spermatogenesis and offers useful resources for understanding differences in sexual function development caused by the change in miRNAs expression between Duroc and Meishan boars.

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“…Sperm motility, kinetics, vigor and hyperactivation levels, are morphofunctional features classically assessed by light microscopy or computer-assisted sperm analysis (i.e., CASA) (Vincent et al, 2012). In addition, since sperm motility requires a substantial amount of energy produced by mitochondrial activity (Piomboni et al, 2012), high fertility samples usually present with high mitochondrial membrane potential. This feature can be assessed by fluorescence microscopy and flow cytometric approaches mainly using the JC-1 fluorescent probe, which forms J-aggregates when mitochondria are active FIGURE 1 | Concept of "healthy (viable) sperm."…”

Section: Morpho-functional Sperm Featuresmentioning

confidence: 99%

From Sperm Motility to Sperm-Borne microRNA Signatures: New Approaches to Predict Male Fertility Potential

Alves

Celeghini

Belleannée

2020

Front. Cell Dev. Biol.

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In addition to the paternal genome, spermatozoa carry several intrinsic factors, including organelles (e.g., centrioles and mitochondria) and molecules (e.g., proteins and RNAs), which are involved in important steps of reproductive biology such as spermatogenesis, sperm maturation, oocyte fertilization and embryo development. These factors constitute potential biomarkers of "viable sperm" and male fertility status and may become major assets for diagnosing instances of idiopathic male infertility in both humans and livestock animals. A better understanding of the mechanism of action of these sperm intrinsic factors in the regulation of reproductive and developmental processes still presents a major challenge that must be addressed. This review assembles the main data regarding morpho-functional and intrinsic sperm features that are associated with male infertility, with a particular focus on microRNA (miRNA) molecules.

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miR‐26a inhibits proliferation and promotes apoptosis in porcine immature Sertoli cells by targeting the PAK2 gene

Cited by 22 publications

References 45 publications

Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars

Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars

Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars

From Sperm Motility to Sperm-Borne microRNA Signatures: New Approaches to Predict Male Fertility Potential

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