miR-27a-5p Attenuates Hypoxia-induced Rat Cardiomyocyte Injury by Inhibiting Atg7

Abstract

Acute myocardial infarction (AMI) is an ischemic heart disease with high mortality worldwide. AMI triggers a hypoxic microenvironment and induces extensive myocardial injury, including autophagy and apoptosis. MiRNAs, which are a class of posttranscriptional regulators, have been shown to be involved in the development of ischemic heart diseases. We have previously reported that hypoxia significantly alters the miRNA transcriptome in rat cardiomyoblast cells (H9c2), including miR-27a-5p. In the present study, we further investigated the potential function of miR-27a-5p in the cardiomyocyte response to hypoxia, and showed that miR-27a-5p expression was downregulated in the H9c2 cells at different hypoxia-exposed timepoints and the myocardium of a rat AMI model. Follow-up experiments revealed that miR-27a-5p attenuated hypoxia-induced cardiomyocyte injury by regulating autophagy and apoptosis via Atg7, which partly elucidated the anti-hypoxic injury effects of miR-27a-5p. Taken together, this study shows that miR-27a-5p has a cardioprotective effect on hypoxia-induced H9c2 cell injury, suggesting it may be a novel target for the treatment of hypoxia-related heart diseases.

Autophagy in cardiovascular diseases: role of noncoding RNAs

“… 21 , 27 miR-27a-5p and miR-188-3p attenuate hypoxia- or A/R-induced cardiomyocyte injury by inhibiting ATG7. 18 , 23 Furthermore, SIRT1 and LC3-II have been recognized as potential targets of miR-204 to mediate the cardioprotective role of miR-204 on I/R-induced cardiomyocytes apoptosis through the repression of autophagy. 15 , 24 …”

Comprehensive Analysis of mRNA and lncRNA Transcriptomes Reveals the Differentially Hypoxic Response of Preadipocytes During Adipogenesis

“…Western blot analysis was performed as previously described (Zhang J. et al, 2019). Total protein was extracted from the hypoxic and normoxic cells in different timepoints using radioimmunoprecipitation assay lysis buffer containing protease and phosphatase inhibitors (Beyotime, Beijing, China) and quantified using a BCA protein assay.…”

Circulating MicroRNAs: Biogenesis and Clinical Significance in Acute Myocardial Infarction

“…Many studies on the diagnostic role of circulating miRNAs in AMI were initially based on big data analysis, but follow-up studies have also confirmed their relationship with cardiovascular disease which we will not discuss it here ( Liu W. L. et al, 2015 ; Wei L. X. et al, 2015 ; Xue et al, 2015 ; He and Huang, 2017 ; Besnier et al, 2019 ; Che et al, 2019 ; Li X. D. et al, 2019 ; Shen et al, 2019 ; Zhang et al, 2019 ). Meder et al (2011) identified two downregulated miRNAs, miR-1291 and miR-663b, among 121 miRNAs related to AMI through whole genome miRNA expression profiling.…”