MiR-27a regulates Wnt/beta-catenin signaling through targeting SFRP1 in glioma

Wang, Kun; Xie, Dajiang; Xie, Jixi; Wan, Yingfeng; Ma, Li; Qi, Xuchen; Yang, Shuxu

doi:10.1097/wnr.0000000000000410

Cited by 29 publications

(34 citation statements)

References 28 publications

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“…Wnt signaling is currently regarded as a significant pathway in carcinogenesis, especially in colorectal cancer; by binding to Wnt molecules, SFRP1 can also act as an antagonist that inhibits Wnt signaling. MiR-27a can regulate the Wnt/β-catenin signaling pathway via the targeting of SFRP1, and after the activation of this signaling pathway, the cell cycle, viability, apoptosis, as well as invasion and migration of cancer cells are affected [32, 42]. Wang K et al have also reported that SFRP1 is a direct target gene of miR-27a and that it can inhibit Wnt signaling through binding to Wnt molecules [42].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-27a Promotes the Proliferation and Invasiveness of Colon Cancer Cells by Targeting SFRP1 through the Wnt/β-Catenin Signaling Pathway

Yao

Long

et al. 2017

Cell Physiol Biochem

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Objective: This study aims to explore the effects of microRNA-27a (miR-27a) on the proliferation and invasiveness of colon cancer cells through the Secreted Frizzled-related protein 1 (SFRP1) and the Wnt/β-catenin signaling pathway. Methods: Colon cancer tissues and adjacent normal tissues from 125 colon cancer patients, together with the HCEpic, HCT-116, HT-29, SW480 and SW620 cell lines, were prepared for this study. The transfected HCT-116 cells were divided into the miR-27a mimics, miR-27a-NC, anti-miR-27a, blank, Lv-SFRP1, Lv-NC, and miR-27a mimics + Lv-SFRP1 groups. RT-qPCR was performed to detect the expressions of miR-27a and SFRP1 mRNA. A dual-luciferase reporter assay was conducted to examine the effect of miR-27a on SFRP1. Western blotting was used to measure the expressions of the SFRP1, β-catenin, GSK-3β, p-β-catenin, p-GSK-3β, c-Myc and cyclin D1 proteins. MTT, soft agar clone formation and Transwell chamber assays were performed to detect cell proliferation and invasion. Results: Compared with normal tissues and cells, colon cancer tissues and cells demonstrated significantly higher expression of miR-27a, but lower expressions of SFRP1 mRNA and protein. MiR-27a negatively regulated the expression of SFRP1 mRNA. SFRP1 was also found to be a target gene of miR-27a. In the miR-27a mimic group, the proliferation and invasiveness of colon cancer cells were significantly increased, while the expressions of GSK-3 β and p-β-catenin were remarkably down-regulated; in contrast, the expressions of p-GSK-3β, -catenin, c-Myc and cyclin D1 were up-regulated. While the proliferation and invasiveness of colon cancer cells in the anti-miR-27a and Lv-SFRP1 groups were decreased, the expressions of GSK-3β and p-β-catenin were elevated, and the expressions of p-GSK-3β, β-catenin, c-Myc and cyclin D1 were decreased. Conclusion: These findings indicated that miR-27a could promote the proliferation and invasiveness of colon cancer cells by targeting SFRP1 through the Wnt/β-catenin signaling pathway.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-27a Promotes the Proliferation and Invasiveness of Colon Cancer Cells by Targeting SFRP1 through the Wnt/β-Catenin Signaling Pathway

Yao

Long

et al. 2017

Cell Physiol Biochem

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“…In non-small cell lung carcinoma, the over-expression of miR-582-3p targets SFRP1 [18]. Studies have also indicated that SFRP1 is a direct target gene of both miR-27a and miR-127-3p [16,19]. In addition, Yang et al discovered that SFRP1 is likely to affect skeletal muscle development and is regulated by miR-1/206 [20].…”

Section: Introductionmentioning

confidence: 99%

“…SFRP1 has also been identified as a tumor-suppressor gene, whose expression is reduced in various malignancies including colorectal, breast and kidney cancer [15,16]. For example, SFRP1 was reduced by 38% on average in patients with bladder cancer [15], and miRNAs are potential regulators of SFRP1.…”

Section: Introductionmentioning

confidence: 99%

MiR-1-3p Suppresses the Proliferation, Invasion and Migration of Bladder Cancer Cells by Up-Regulating SFRP1 Expression

Shang

Yang

Shen

et al. 2017

Cell Physiol Biochem

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Key WordsMiR-1-3p • SFRP1 • bladder cancer Abstract Background: Bladder cancer is of compelling morbidity and mortality due to its high recurrence rate. Little development has been made in the last decades in the therapy methods. Thus, the mechanism of its growth and invasiveness involving novel molecular targets are needed. Objective: Our research objective is to confirm the hypothesis that miR-1-3p suppresses the proliferation, invasion and migration of bladder cancer cells. Methods: The expression levels of miR-1-3p and SFRP1 were evaluated using RT-qPCR in bladder cancer tissues and cells as well as in normal tissues and cells. J82 cell lines were selected as experiment subjects due to their low expression levels of miR-1-3p. Plasmids carrying miR-1-3p mimics, miR-1-3p inhibitors and SFRP1 were transfected into the J82 cell lines. Subsequently, the protein expression of SFRP1 was detected using Western Blot analysis, and cell proliferation, apoptosis, invasion and migration ability was measured using MTT, the flow cytometry, the Transwell test and wound healing assays, respectively. Results: Bladder cancer tissues and cells exhibited significant decrease in the expression of miR-1-3p and SFRP1 compared to normal tissues and cells, and human bladder cancer cell line J82 exhibited the most significant decrease in these expressions (P < 0.05). MiR-1-3p up-regulates SFRP1 expression in bladder cancer cells, and the over-expression of miR-1-3p can suppress the proliferation, invasion and migration ability of bladder cancer cells. This mechanism is similar to the effect of SFRP1 over-expression on bladder cancer cells. Conclusion: MiR-1-3p suppresses the proliferation, invasion and migration of bladder cancer cells by up-regulating SFRP1 expression.

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“…Once in the nucleus, b-catenin stimulates the transcription of target genes, thus promoting predominantly glioma cell proliferation via TCF/LEF family of transcription factors (13). If the Wnt/ b-catenin pathway is blocked, an antineoplastic response is normally observed (14). Most notably, noncanonical Wnts such as Wnt2, Wnt4, Wnt5a, Wnt6, and Wnt11 modulate predominantly cell movement and polarity through the activation of b-catenin-independent pathways (15).…”

Section: Introductionmentioning

confidence: 99%

Wnt5a Drives an Invasive Phenotype in Human Glioblastoma Stem-like Cells

et al. 2017

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Brain invasion by glioblastoma determines prognosis, recurrence, and lethality in patients, but no master factor coordinating the invasive properties of glioblastoma has been identified. Here we report evidence favoring such a role for the noncanonical WNT family member Wnt5a. We found the most invasive gliomas to be characterized by Wnt5a overexpression, which correlated with poor prognosis and also discriminated infiltrating mesenchymal glioblastoma from poorly motile proneural and classical glioblastoma. Indeed, Wnt5a overexpression associated with tumor-promoting stem-like characteristics (TPC) in defining the character of highly infiltrating mesenchymal glioblastoma cells (Wnt5a High ). Inhibiting Wnt5a in mesenchymal glioblastoma TPC suppressed their infiltrating capability. Conversely, enforcing high levels of Wnt5a activated an infiltrative, mesenchymal-like program in classical glioblastoma TPC and Wnt5aLow mesenchymal TPC. In intracranial mouse xenograft models of glioblastoma, inhibiting Wnt5a activity blocked brain invasion and increased host survival. Overall, our results highlight Wnt5a as a master regulator of brain invasion, specifically TPC, and they provide a therapeutic rationale to target it in patients with glioblastoma. Cancer Res; 77(4); 996-1007. Ó2016 AACR.

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MiR-27a regulates Wnt/beta-catenin signaling through targeting SFRP1 in glioma

Cited by 29 publications

References 28 publications

MicroRNA-27a Promotes the Proliferation and Invasiveness of Colon Cancer Cells by Targeting SFRP1 through the Wnt/β-Catenin Signaling Pathway

MicroRNA-27a Promotes the Proliferation and Invasiveness of Colon Cancer Cells by Targeting SFRP1 through the Wnt/β-Catenin Signaling Pathway

MiR-1-3p Suppresses the Proliferation, Invasion and Migration of Bladder Cancer Cells by Up-Regulating SFRP1 Expression

Wnt5a Drives an Invasive Phenotype in Human Glioblastoma Stem-like Cells

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