miR-29a inhibits human retinoblastoma progression by targeting STAT3

Liu, Shu; Zhang, Xiaomeng; Hu, Chunmei; Wang, Yingxue; Xu, Chunling

doi:10.3892/or.2017.6144

Cited by 20 publications

(17 citation statements)

References 24 publications

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“…miRNAs may act as tumour suppressors or oncogenes, in which aberrantly downregulated miRNAs play tumour-suppressive roles, whereas highly expressed miRNAs serve oncogenic roles in carcinogenesis and cancer progression (12). Emerging studies have indicated that numerous miRNAs are dysregulated in RB, such as miR-29a (13), miR-320 (14), miR-613 (15) and miR-143 (16). Aberrantly expressed miRNAs are closely related with RB formation and progression by regulating various pathological behaviours, such as cellular proliferation, apoptosis, invasion, metastasis and angiogenesis (17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑758 inhibits malignant progression of retinoblastoma by directly targeting PAX6

You

2018

Oncol Rep

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Accumulated studies have highlighted that the dysregulation of microRNAs (miRNAs) in retinoblastoma (RB) is a leading cause for tumourigenesis and tumour development. Therefore, the elucidation of the expression, functional roles and underlying mechanisms of miRNAs in RB will help the development of promising therapeutic methods to improve the prognosis of RB patients. The aim of this study was to detect miRNA‑758 (miR‑758) expression in RB tissues and cell lines, and to determine the effects and underlying mechanisms of miR‑758 on RB progression. The results demonstrated that miR‑758 was downregulated in both RB tissues and cell lines. In vitro functional experiments revealed that upregulation of miR‑758 inhibited cell proliferation, migration and invasion, and induced apoptosis in RB. In addition, paired box protein 6 (PAX6) was a direct target gene of miR‑758 in RB. Furthermore, PAX6 was upregulated in RB tissues, and this upregulation was inversely associated with the expression level of miR‑758. In addition, PAX6 reintroduction abrogated the tumour‑suppressive effects of miR‑758 overexpression on RB cell proliferation, migration, invasion and apoptosis. Furthermore, miR‑758 overexpression inactivated the PI3K/Akt pathway in RB cells by inhibiting PAX6. In conclusion, our current study provided sufficient evidence to demonstrate that miR‑758 inhibits the progression of RB by directly targeting PAX6 and regulating the PI3K/Akt pathway, thereby suggesting that this miRNA may be developed as a therapeutic target for treating patients with RB.

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Section: Introductionmentioning

confidence: 99%

MicroRNA‑758 inhibits malignant progression of retinoblastoma by directly targeting PAX6

You

2018

Oncol Rep

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“…Numerous reports have demonstrated that miRNAs are involved in a variety of physiological processes, including cell proliferation, development, survival and differentiation (7)(8)(9)(10). Accumulating studies have also indicated that miRNAs may serve as oncogenes or tumor suppressors to regulate tumor cell proliferation, migration and invasion (11)(12)(13)(14). For instance, miRNA-320a inhibits tumor proliferation and invasion by targeting c-Myc in human hepatocellular carcinoma (15).…”

Section: Introductionmentioning

confidence: 99%

“…For instance, miRNA-320a inhibits tumor proliferation and invasion by targeting c-Myc in human hepatocellular carcinoma (15). Numerous miRNAs have been reported to regulate the progression of RB, including miR-29a (12), miR106b (13), miRNA-382 (16) and miRNA-320 (17); however, the functions of miRNAs in RB are yet to be determined and the roles of most miRNAs in RB remain unknown.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑93‑5p promotes the progression of human retinoblastoma by regulating the PTEN/PI3K/AKT signaling pathway

et al. 2018

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Numerous repor ts have indicated that microRNA-93-5p (miR-93-5p) is involved in the development and progression of human cancer, including non-small cell lung, gastric and breast cancer; however, the role of miR-93-5p in retinoblastoma (RB) remains unknown. In the present study, it was reported that miR-93-5p expression levels were significantly upregulated in RB tissues compared with in normal tissues by reverse transcription-quantitative polymerase chain reaction. Furthermore, it was demonstrated via cell counting kit-8 and Transwell assays that knockdown of miR-93-5p significantly suppressed the proliferation, migration and invasion of RB cells, but promoted cellular apoptosis. Regarding the underlying mechanism, the present study reported that phosphatase and tensin homolog (PTEN) was a direct target of miR-93-5p in RB cells. Overexpression of miR-93-5p significantly inhibited the expression of PTEN; opposing results were observed when PTEN expression was downregulated. Furthermore, the present study revealed that PTEN expression levels were downregulated and were inversely correlated with that of miR-93-5p in RB tissues. Additionally, the present study demonstrated that knockdown of PTEN in miR-93-5p-depleted RB cells significantly reversed the effects of miR-93-5p on cell proliferation, migration and invasion; miR-93-5p knockdown was suggested to promote PTEN expression, consequently inhibiting the activation of phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway. Collectively, the results of the present study demonstrated that miR-93-5p may serve a role as an oncogene by modulating the PTEN/PI3K/AKT signaling pathway in RB, indicating that miR-93-5p may be a potential therapeutic target for the treatment of RB.

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“…Over the past decade, studies have revealed that miRNAs are altered in nearly all human cancer types, and that their alteration contributes toward the initiation and progression of cancer (10)(11)(12). An increasing volume of evidence has recently indicated that numerous miRNAs, including miR-29a (13), miR-138 (14), miR-498 (15) and miR-655 (16), are aberrantly expressed in RB. miRNA dysregulation participates in the regulation of various biological behaviours, including cellular proliferation, cycle, apoptosis and invasion, as well as metastasis and angiogenesis (17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of microRNA‑492 attenuates cell proliferation and invasion in retinoblastoma via directly targeting LATS2

Sun

Zhang

2018

Mol Med Report

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Numerous studies have demonstrated that microRNAs (miRNAs) are upregulated or downregulated in retinoblastoma (RB), and that this phenomenon is associated with the modulation of various malignant behaviours during RB occurrence and development. Therefore, the mechanisms that associate deregulated miRNAs with RB initiation and progression must be understood to identify effective therapeutic techniques for patients with RB. In the present study, miR-492 expression was upregulated in RB tissues and cell lines. The effects of miR-492 inhibition on the proliferation and invasion of RB cells were examined using Cell Counting kit-8 and invasion assays. The results revealed that miR-492 downregulation significantly decreased the proliferation and invasion of RB cells. Bioinformatics analysis predicted that large tumour-suppressor kinase 2 (LATS2) was a putative target of miR-492. Luciferase reporter assay, reverse transcription-quantitative polymerase chain reaction and western blot analysis demonstrated that LATS2 was a direct target gene of miR-492 in RB cells. In addition, LATS2 expression was downregulated in RB tissues, and its downregulation was inversely correlated with miR-492 level. Furthermore, LATS2-knockdown abrogated the effects of miR-492 downregulation in RB cells. In conclusion, miR-492 inhibition may impede the malignant behaviour of RB by directly targeting LATS2. Therefore, targeting this miRNA may be an effective therapeutic method for treating patients with RB.

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miR-29a inhibits human retinoblastoma progression by targeting STAT3

Cited by 20 publications

References 24 publications

MicroRNA‑758 inhibits malignant progression of retinoblastoma by directly targeting PAX6

MicroRNA‑758 inhibits malignant progression of retinoblastoma by directly targeting PAX6

MicroRNA‑93‑5p promotes the progression of human retinoblastoma by regulating the PTEN/PI3K/AKT signaling pathway

Inhibition of microRNA‑492 attenuates cell proliferation and invasion in retinoblastoma via directly targeting LATS2

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