Mir-29A Inhibits Invasion and Metastasis of Cervical Cancer Via Modulating Methylation of Tumor Suppressor Socs1

Abstract

Aims: To investigate roles of miR-29a-DNMT1-SOCS1 axis in cervical cancer invasion and migration. Materials & methods: The methylation level of SOCS1 was determined by methylation specific PCR. The cell apoptosis, proliferation, migration and invasion were examined by Annexin-V/PI staining, MTT and colony formation assays, plus scratch and transwell assays respectively. The expressions of epithelial–mesenchymal transition and NF-κB related proteins were determined by western blotting. Results: MiR-29a was downregulated, accompanied with DNMT1 upregulation and SOCS1 downregulation in cervical cancer tissues. MiR-29a suppressed DNMT1, inhibited SOCS1 promoter methylation and upregulated its expression. Moreover, miR-29a promoted cell apoptosis, suppressed proliferation, inhibited migration and invasion via inactivation of NF-κB signaling pathway in cervical cancer cells. Conclusion: MiR-29a-DNMT1-SOCS1 axis plays an important role on invasion and metastasis in cervical cancer via NF-κB signaling pathway.

LINC00636 promotes lymph node metastasis and cervical cancer through targeting NM23

“…Cervical cancer is the third most common cancer and the second leading cause of cancer-related deaths in women worldwide [ 2 , 3 ]. Metastasis is one major challenge for cervical cancer treatment, as most cervical cancer-related mortality are caused by metastasis [ 4 ]. Unfortunately, there are currently no effective treatment options for preventing or inhibiting cervical cancer metastasis, in part because the mechanisms that underlie cervical cancer metastasis are not fully defined.…”

miR-29a-3p directly targets Smad nuclear interacting protein 1 and inhibits the migration and proliferation of cervical cancer HeLa cells

“…In contrast, other studies have shown that miR-29a was down-regulated and inhibited the progression of cancers such as colon cancer ( Shi et al, 2020 ), non-small cell lung cancer ( Hu et al, 2016 ) and adenocarcinoma ( Zhang et al, 2018 ). Previous studies have shown that miR-29a was low expression in cervical cancer, as a tumor suppressor, miR-29a can target multiple genes, such as CDC42, HSP47, SIRT1 and DNMT1 ( Park et al, 2009 ; Yamamoto et al, 2013 ; Gong et al, 2019 ; Nan et al, 2019 ). Nonetheless, the detailed biological function of miR-29a in cervical cancer has not yet been completely revealed.…”

“…As oncogene or tumor suppressor in different cancers, miRNAs play essential roles in basic biological processes such as cell proliferation, apoptosis, differentiation, migration and invasion ( Bartel, 2004 ; Pardini et al, 2018 ). Accumulating studies have shown that miR-29a was abnormally expressed in various cancers, including cervical cancer ( Pei, Lei & Liu, 2016 ; Yang et al, 2017 ; Gong et al, 2019 ). However, the detailed role and underlying mechanism of miR-29a in cervical cancer is still largely unclear.…”

Peer Review #2 of "miR-29a-3p directly targets Smad nuclear interacting protein 1 and inhibits the migration and proliferation of cervical cancer HeLa cells (v0.1)"

“…And the knockdown of SNIP1 restrained the anchorageindependent growth of lung cancer cells (Jeon et al, 2013). However, the function of SNIP1 in (Park et al, 2009;Yamamoto et al, 2013;Gong et PeerJ reviewing PDF | (2020:06:49760:1:0:NEW 14 Sep 2020)…”

“…Accumulating studies have shown that miR-29a was abnormally expressed in various cancers, including cervical cancer (Pei, Lei, & Liu, 2016;Yang et al, 2017;Gong et al, 2019). However, the detailed role and underlying mechanism of miR-29a in cervical cancer is still largely unclear.…”