MiR-29a suppresses cell proliferation by targeting SIRT1 in hepatocellular carcinoma

Zhang, Yongyu; Wang, Shiji; Liu, Zhongmin; Xiu, Ming

doi:10.3233/cbm-171120

Cited by 30 publications

(32 citation statements)

References 18 publications

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“…In fact, miR-29a and miR-29c are members of the miR-29 family, which is known to be associated with tumorigenesis and cancer progression [29]. MiR-29a might act as either an oncogene or tumor suppressor in a wide variety of malignancies [30]. Besides, numerous studies [4,5] have identified the significant upregulation of miR-29a in serum and plasma from CRC patients compared to controls, as we have demonstrated in the current study.…”

Section: Discussionsupporting

confidence: 71%

A Novel Saliva-Based miRNA Signature for Colorectal Cancer Diagnosis

Rapado‐González

Majem

Álvarez-Castro

et al. 2019

JCM

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Salivary microRNAs (miRNAs) are of high interest as diagnostic biomarkers for non-oral cancer. However, little is known about their value for colorectal cancer (CRC) detection. Our study aims to characterize salivary miRNAs in order to identify non-invasive markers for CRC diagnosis. The screening of 754 miRNAs was performed in saliva samples from 14 CRC and 10 healthy controls. The differential expressed miRNAs were validated by RT-qPCR in 51 CRC, 19 adenomas and 37 healthy controls. Receiver operating characteristic (ROC) curves and logistic regression models were performed to analyze the clinical value of these miRNAs. Twenty-two salivary miRNAs were significantly deregulated in CRC patients vs. healthy individuals (P < 0.05) in the discovery phase. From those, five upregulated miRNAs (miR-186-5p, miR-29a-3p, miR-29c-3p, miR-766-3p, and miR-491-5p) were confirmed to be significantly higher in the CRC vs. healthy group (P < 0.05). This five-miRNA signature showed diagnostic value (72% sensitivity, 66.67% specificity, AUC = 0.754) to detect CRC, which was even higher in combination with carcinoembryonic antigen (CEA) levels. Overall, after the first global characterization of salivary miRNAs in CRC, a five-miRNA panel was identified as a promising tool to diagnose this malignancy, representing a novel approach to detect cancer-associated epigenetic alterations using a non-invasive strategy.

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Section: Discussionsupporting

confidence: 71%

A Novel Saliva-Based miRNA Signature for Colorectal Cancer Diagnosis

Rapado‐González

Majem

Álvarez-Castro

et al. 2019

JCM

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Section: Introductionmentioning

confidence: 99%

“…Several studies have shown that miR-29a played an important role in HCC cells [8,9]. MiR-29a could reduce the proliferation of HCC cells by targeting silent mating type information regulation 2 homolog-1 (SIRT1) [8]. MiR-29a inhibited the expression of recombinant claudin 1 (CLDN1) to reduce the migration and proliferation of HCC cells [9].…”

Section: Introductionmentioning

confidence: 99%

miR-29a overexpresion induces apoptosis through targeting VEGF in hepatocellular carcinoma

Wang¹,

Wu²,

Yuan³

et al. 2020

Preprint

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Background : This study aimed to explore the role of miR-29a in hepatocellular carcinoma (HCC) cells. Further, to confirm whether miR-29a targetes vascular endothelial growth factor (VEGF) in HCC cells. Methods: Cell counting Kit-8 (CCK8) and clone formation assay were used to analyze the proliferation of HCC cells. Flow cytometry was performed to analyze the apoptosis and cell cycle of HCC cells. Western blot was used to analyze the expression of Bax, B-cell lymphoma-2 (Bcl-2), cyclin dependent kinase 4 (CDK4), CyclinD1, Retinoblastoma (Rb), p53 and myeloid cell leukemia-1 (MCL1). The targeting relations between miR-29a and VEGF was measuered by dual luciferase assay. Results : Overexpression of miR-29a could distinctly inhibit the proliferation and promote apoptosis of HCC cells. Overexpression of miR-29a obviously up-regulated the expressions of Bax and Rb, and reduced the expressions of CDK4, Bcl-2, Cyclin D1, p53, MCL1 and VEGF in HCC cells. Dual luciferase assay proved that VEGF was the target of miR-29a. Rescue experiments showed that miR-29a targeted VEGF to regulate the proliferation and apoptosis in HCC cells. Conclusions : These results indicated overexpression of miR-29a inhibited the HCC cells proliferation through targeting VEGF.

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“…miRNAs are differently expressed in a number of types of human cancer, including HCC (11), and ovarian (12), thyroid (13) and gastric cancer (14). Dysregulated miRNAs serve a pivotal role in numerous biological behaviors, including cell proliferation, cell cycle, apoptosis, metastasis and angiogenesis (15)(16)(17). miRNAs are able to serve tumor-suppressing or oncogenic roles in the formation and progression of HCC, which primarily depends on the characteristic of their target genes (18,19).…”

Section: Introductionmentioning

confidence: 99%

“…miRNAs are able to serve tumor-suppressing or oncogenic roles in the formation and progression of HCC, which primarily depends on the characteristic of their target genes (18,19). For example, miR-29a inhibits HCC cell proliferation, colony formation and inhibits cell cycle progression by directly targeting Sirtuin 1 (15). miR-1468 is upregulated in HCC and promotes aggressive tumor behaviors by activating peroxisome proliferator-activated receptor gamma-mediated protein B kinase signaling (20).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma

Guo

Miao

et al. 2019

Mol Med Report

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Previous studies have demonstrated that numerous tumor-specific microRNAs (miRNAs) are upregulated or downregulated in hepatocellular carcinoma (HCC), and that their dysregulation is implicated in HCC occurrence and development. Therefore, investigation of crucial miRNAs involved in HCC oncogenesis and progression may provide novel insights into the therapy of patients with this malignant tumor. In the present study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays were performed to detect tissue and cellular expression levels of miRNA-663b (miR-663b) in HCC. The effects of miR-663b overexpression on the proliferation and invasion of HCC cells were examined using Cell Counting Kit-8 and Transwell invasion assays, respectively. The direct target of miR-663b in HCC cells was determined by bioinformatics analysis, luciferase reporter assay, RT-qPCR and western blot analysis. It was observed that miR-663b was expressed at low levels in HCC tissues and cell lines. miR-663b upregulation suppressed the proliferative and invasive abilities of HCC cells. Additionally, Grb2-associated binding 2 (GAB2) was regarded as a direct target gene of miR-663b in HCC cells. Furthermore, GAB2 was overexpressed in HCC tissues, and overexpression of GAB2 was inversely correlated with levels of miR-663b. GAB2 overexpression was able to rescue the suppressive effects of miR-663b on HCC cells. These results demonstrated that this newly-identified miR-663b/GAB2 axis may be implicated in HCC occurrence and development.

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MiR-29a suppresses cell proliferation by targeting SIRT1 in hepatocellular carcinoma

Abstract: Thus, these results indicated that miR-29a may serve as a potential target of HCC treatment.

Cited by 30 publications

References 18 publications

A Novel Saliva-Based miRNA Signature for Colorectal Cancer Diagnosis

A Novel Saliva-Based miRNA Signature for Colorectal Cancer Diagnosis

miR-29a overexpresion induces apoptosis through targeting VEGF in hepatocellular carcinoma

MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma

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