MiR-29b is associated with perinatal inflammation in extremely preterm infants

Pavlek, Leeann R.; Vudatala, Sundari; Bartlett, Christopher W.; Buhimschi, Irina A.; Buhimschi, Catalin S.; Rogers, Lynette K.

doi:10.1038/s41390-020-0943-1

Cited by 9 publications

(12 citation statements)

References 41 publications

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“…It has also been shown that the miRNA profiles of the placenta 31 , amniotic fluid 34 , and maternal serum 32 , whole blood and monocytes 35 differ between preterm and full term births. Although the lasting effect on PTB on DNA methylation pattern 19,36 and subjects' health 37 have been studied, only a few studies analyzing the associations of PTB with the subjects' circulatory miRNA profile have been conducted on newborns [38][39][40][41] and one case-control study (n = 50) with 4 selected miRNAs in young adults 42 .…”

mentioning

confidence: 99%

Adulthood blood levels of hsa-miR-29b-3p associate with preterm birth and adult metabolic and cognitive health

Marttila

Rovio

Mishra

et al. 2021

Sci Rep

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Preterm birth (PTB) is associated with increased risk of type 2 diabetes and neurocognitive impairment later in life. We analyzed for the first time the associations of PTB with blood miRNA levels in adulthood. We also investigated the relationship of PTB associated miRNAs and adulthood phenotypes previously linked with premature birth. Blood MicroRNA profiling, genome-wide gene expression analysis, computer-based cognitive testing battery (CANTAB) and serum NMR metabolomics were performed for Young Finns Study subjects (aged 34–49 years, full-term n = 682, preterm n = 84). Preterm birth (vs. full-term) was associated with adulthood levels of hsa-miR-29b-3p in a fully adjusted regression model (p = 1.90 × 10–4, FDR = 0.046). The levels of hsa-miR-29b-3p were down-regulated in subjects with PTB with appropriate birthweight for gestational age (p = 0.002, fold change [FC] = − 1.20) and specifically in PTB subjects with small birthweight for gestational age (p = 0.095, FC = − 1.39) in comparison to individuals born full term. Hsa-miR-29b-3p levels correlated with the expressions of its target-mRNAs BCL11A and CS and the gene set analysis results indicated a target-mRNA driven association between hsa-miR-29b-3p levels and Alzheimer's disease, Parkinson's disease, Insulin signaling and Regulation of Actin Cytoskeleton pathway expression. The level of hsa-miR-29b-3p was directly associated with visual processing and sustained attention in CANTAB test and inversely associated with serum levels of VLDL subclass component and triglyceride levels. In conlcusion, adult blood levels of hsa-miR-29b-3p were lower in subjects born preterm. Hsa-miR-29b-3p associated with cognitive function and may be linked with adulthood morbidities in subjects born preterm, possibly through regulation of gene sets related to neurodegenerative diseases and insulin signaling as well as VLDL and triglyceride metabolism.

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confidence: 99%

Adulthood blood levels of hsa-miR-29b-3p associate with preterm birth and adult metabolic and cognitive health

Marttila

Rovio

Mishra

et al. 2021

Sci Rep

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“…miR-29a-3p has been reported in a multitude of diseases, ranging from intrauterine inflammation in extremely preterm infants ( 15 ) and airway epithelial remodeling in chronic obstructive pulmonary disease ( 16 ) to proliferation and differentiation in retinal progenitors ( 17 ). Studies have reported that miR-29a-3p could be a therapeutic tool for certain diseases, such as pulmonary fibrosis ( 14 ) and Alzheimer's disease ( 18 ).…”

Section: Discussionmentioning

confidence: 99%

miR‑29a‑3p regulates the epithelial‑mesenchymal transition via the SPARC/ERK signaling pathway in human bronchial epithelial cells

et al. 2021

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Neutrophilic asthma (NA) is a subtype of asthma that responds poorly to corticosteroid treatment. In certain diseases, microRNA (miR)-29a-3p is considered to be a key regulatory molecule for remodeling of the extracellular matrix. However, the effect of miR-29a-3p on airway remodeling is unknown. The present study aimed to investigate the role of miR-29a-3p in NA. A mouse model of NA was established and these animals were compared to normal controls. Both groups of mice were subjected to lung function tests and histopathological analysis. Human bronchial epithelial cells (16HBE) were grown in culture and incubated with secreted protein acidic rich in cysteine (SPARC) and a miR-29a-3p mimic. The expression of miR-29a-3p, SPARC and epithelial-mesenchymal transition (EMT)-related markers were measured using reverse transcription-quantitative PCR and western blotting. Luciferase reporter assay was performed to identify the direct regulatory relationship between miR-29a-3p and SPARC. miR-29a-3p expression was significantly decreased, while SPARC expression was increased in the NA mouse model with a phenotype of EMT. Overexpression of SPARC downregulated the expression of E-cadherin, while it increased the expression of vimentin in 16HBE cells. miR-29a-3p administration reversed the SPARC-induced effects on E-cadherin and vimentin expression. Luciferase assays confirmed that SPARC was the target gene for miR-29a-3p. Furthermore, SPARC overexpression increased the protein expression of phosphorylated (p)-ERK, while transfection with miR-29a-3p mimics significantly inhibited this increase. The data suggested that EMT in the NA mouse model was associated with decreased levels of miR-29a-3p and elevated SPARC. Furthermore, SPARC could induce the formation of EMT in 16HBE cells in vitro and this was directly targeted by miR-29a-3p and mediated by p-ERK, suggesting that miR-29a-3p may participate in the airway remodeling of NA.

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“…MicroRNAs (miRs) are small noncoding RNAs of about 22 nucleotides that are implicated in developmental processes such as neurogenesis and myogenesis, as well as in many pathological processes [105]. Amongst all noncoding RNAs, miRNAs are the most well studied for their regulatory roles in controlling gene expression by either targeting mRNAs for degradation or blocking their translation.…”

Section: Micrornas In Cpmentioning

confidence: 99%

“…Furthermore, miR-200c overexpression inhibited skeletal muscle differentiation [114]. Satellite-cell proliferation is stimulated by the overexpression of miR-29c, which represses the expression of the atrophy-related genes, implying an association between lower miR-29 levels and CP [105,115]. This miRNA has been implicated in regulation of extracellular matrix deposition and its expression may be inhibited as a result of inflammation.…”

Section: Micrornas In Cpmentioning

confidence: 99%

An Emerging Role for Epigenetics in Cerebral Palsy

Romero

Robinson

Batish

et al. 2021

JPM

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Cerebral palsy is a set of common, severe, motor disabilities categorized by a static, nondegenerative encephalopathy arising in the developing brain and associated with deficits in movement, posture, and activity. Spastic CP, which is the most common type, involves high muscle tone and is associated with altered muscle function including poor muscle growth and contracture, increased extracellular matrix deposition, microanatomic disruption, musculoskeletal deformities, weakness, and difficult movement control. These muscle-related manifestations of CP are major causes of progressive debilitation and frequently require intensive surgical and therapeutic intervention to control. Current clinical approaches involve sophisticated consideration of biomechanics, radiologic assessments, and movement analyses, but outcomes remain difficult to predict. There is a need for more precise and personalized approaches involving omics technologies, data science, and advanced analytics. An improved understanding of muscle involvement in spastic CP is needed. Unfortunately, the fundamental mechanisms and molecular pathways contributing to altered muscle function in spastic CP are only partially understood. In this review, we outline evidence supporting the emerging hypothesis that epigenetic phenomena play significant roles in musculoskeletal manifestations of CP.

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MiR-29b is associated with perinatal inflammation in extremely preterm infants

Cited by 9 publications

References 41 publications

Adulthood blood levels of hsa-miR-29b-3p associate with preterm birth and adult metabolic and cognitive health

Adulthood blood levels of hsa-miR-29b-3p associate with preterm birth and adult metabolic and cognitive health

miR‑29a‑3p regulates the epithelial‑mesenchymal transition via the SPARC/ERK signaling pathway in human bronchial epithelial cells

An Emerging Role for Epigenetics in Cerebral Palsy

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