MiR-29c-3p and MiR-223-3p Regulate the Proliferation and Drug Resistance of OSCC by Targeting ANGPTL4

Abstract

BackgroundOral cancer is one of the most common malignant tumors of the head and neck. MicroRNA are reported to be involved in the regulation of posttranscriptional gene expression. The aim of this research is to determine the expression and function of ANGPTL4 in OSCC, and to explore whether miR-29C-3p and miR-223-3p targeting ANGPTL4 regulate the proliferation and cisplatin resistance of OSCC.MethodsA bioinformatics database was used to identify the expression of ANGPTL4 and miRNAs in tumors, and qPCR and WB were used to determine the protein and mRNA expression of ANGPTL4 and miRNA in tissues and cells. The proliferation of the cells was determined by the plate colony formation and CCK-8 assays, the drug resistance of the cells was determined by IC50 measurements, and a dual luciferase reporter assay was used to determine the regulation of miRNA on ANGPTL4. Independent sample t test was used for the parameters that followed a normal distribution, otherwise, non-parametric test was used. ResultsANGPTL4 expression was higher in the OSCC tissues than in the normal tissues. After knocking down ANGPTL4, the proliferation of OSCC cells decreased, and their sensitivity to cisplatin increased. A dual luciferase reporting assay showed that the fluorescence intensity of the WT group decreased after miR-29c-3p and miR-223-3p were overexpression, while that of the MUT group was almost unchanged. The overexpression of miR-29c-3p and miR-223-3p decreased the protein levels of ANGPTL4. In addition, the proliferation of OSCC cells decreased, and cell resistance to cisplatin was decreased.ConclusionmiR-29c-3p and miR-223-3p can regulate cell proliferation and cisplatin resistance in OSCC by targeting ANGPTL4.