MiR-29c inhibits cell growth, invasion, and migration of pancreatic cancer by targeting ITGB1

Lu, Yao; Hu, Juanjuan; Sun, Weijia; Li, Shengyu; Deng, Shuangya; Li, Ming

doi:10.2147/ott.s92758

Cited by 33 publications

(19 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, miRNAs have the potential to serve as novel biomarkers for cancer diagnosis and prognosis prediction, as they remain largely intact. Several miRNAs have been reported to serve vital roles in the tumorigenesis and prognosis of PAAD ( 16 – 21 ). However, the associations between other miRNAs and the survival of patients with PAAD remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Prognostic microRNAs and their potential molecular mechanism in pancreatic cancer: A study based on The Cancer Genome Atlas and bioinformatics investigation

Liang

Wei

et al. 2017

Mol Med Report

View full text Add to dashboard Cite

Although certain biomarkers that are directly associated with the overall survival (OS) of patients with pancreatic adenocarcinoma (PAAD) have been identified, the efficacy of a single factor is limited to predicting the prognosis. The aim of the present study was to identify a combination micro (mi)RNA signature that enhanced the prognostic prediction for PAAD. Following analysis of the data available from The Cancer Genome Atlas (TCGA), 175 PAAD samples were selected for the present study, and the associations between 494 miRNAs and OS were investigated. The prognostic value of all miRNAs was analyzed by multivariate Cox regression, and the miRNAs were ranked according to the hazard ratio (HR) and P-values. The top 5 miRNAs (miR-1301, miR-125a, miR-376c, miR-328 and miR-376b) were significantly associated with OS (HR=0.139; 95% confidence interval, 0.043–0.443; P<0.001), thus demonstrating that this panel was able to serve as an independent prognostic factor for PAAD. In addition, the present study also predicted the target genes of the top 10 miRNAs with the highest prognostic values using 12 different prediction software, and enrichment signaling pathway analyses elucidated that several pathways may be markedly associated with these miRNAs, including ‘Pathways in cancer’, ‘Chronic myeloid leukemia’, ‘Glioma’ and ‘MicroRNAs in cancer’. Lastly, ubiquitin C, epidermal growth factor receptor, estrogen receptor 1, mitogen-activated protein kinase 1, mothers against decapentaplegic homolog 4 and androgen receptor may be the hub genes revealed by STRING analysis. The present study identified several miRNAs, particularly a five-miRNA-pool, that may be reliable, independent factors for predicting survival in patients with PAAD. However, the underlying molecular mechanisms require further investigation in the future.

show abstract

Section: Introductionmentioning

confidence: 99%

Prognostic microRNAs and their potential molecular mechanism in pancreatic cancer: A study based on The Cancer Genome Atlas and bioinformatics investigation

Liang

Wei

et al. 2017

Mol Med Report

View full text Add to dashboard Cite

show abstract

“…MiR-29c plays the role as tumor suppressor in several kinds of tumors. MiR-29c was shown to inhibit cell growth, cell migration and invasion in pancreatic cancer by targeting ITGB1 [ 5 ]. In bladder cancer, miR-29c overexpression inhibited cell growth, suppressed cell migration and resulted in an accumulation of cells in the G1 phase during the cell cycle through the target gene CDK6 [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

microRNA-29c inhibits cell proliferation by targeting NASP in human gastric cancer

Chen

et al. 2017

BMC Cancer

View full text Add to dashboard Cite

BackgroundGastric cancer is one of the most common malignancies worldwide. Recent studies have shown that microRNAs play crucial roles in regulating cellular proliferation process in gastric cancer. MicroRNA-29c (miR-29c) acts as a tumor suppressor in different kinds of tumors.MethodsQuantitative PCR was performed to evaluate miR-29c expression level in 67 patient gastric cancer tissues and 9 gastric cancer cell lines. The effects of miR-29c were explored by proliferation assay, soft agar colony formation assay, apoptosis and cell cycle analysis using flow cytometry. The target gene was predicted by bioinformatic algorithms and validated by dual luciferase reporter assay and Western blot analysis.ResultsIn this study, we demonstrate that miR-29c is down-regulated in gastric cancer tissues and cell lines. We indicate that overexpression of miR-29c inhibits cell proliferation, promotes apoptosis and arrests cell cycle at G1/G0 phase. We additionally show that miR-29c exerts these effects by targeting Nuclear autoantigenic sperm protein (NASP). Moreover, depletion of NASP can elite the phenotypes caused by miR-29c.ConclusionsThese data suggest that miR-29c inhibits proliferation in gastric cancer and could potentially serve as an early biomarker and a novel therapy target.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-017-3096-9) contains supplementary material, which is available to authorized users.

show abstract

“…Targeting TKT has been demonstrated to lead to increased oxidative stress, causing increased sensitivity of cancer cells to therapeutic treatments, including Sorafenib ( 29 ). ATP binding cassette subfamily C member 1 ( 30 ), integrin subunit β1 (ITGB1) ( 31 , 32 ) and aldo-keto reductase family 1 member B ( 33 , 34 ) have been reported to be involved in chemoresistance/radioresistance and cancer metastasis. P21-activated kinase 2 ( 35 ), G protein nucleolar 3 ( 36 ), nucleophosmin ( 37 ) and pericentriolar material 1 ( 38 ) have been associated with metastatic characteristics in various types of cancer.…”

Section: Resultsmentioning

confidence: 99%

Comparative proteomics of side population cells derived from human hepatocellular carcinoma cell lines with varying metastatic potentials

et al. 2018

View full text Add to dashboard Cite

Metastasis and recurrence following surgery are major reasons for the high mortality rate and poor prognosis associated with hepatocellular carcinoma (HCC). Cancer stem cells (CSCs) are thought to be able to cause cancer, and to be the primary cause of tumor recurrence and metastasis. The underlying mechanisms of the metastatic potential of CSCs is poorly understood. In the present study, side population (SP) cells were isolated from 4 HCC cell lines, and their self-renewal and migratory abilities were compared. The results demonstrate that SP cells from different cell lines exhibited similar self-renewal abilities but different metastatic potentials. Furthermore, the overall proteomes of the SP cells were systematically quantified. This revealed 11 and 19 differentially expressed proteins (DEPs), upregulated and downregulated, respectively, associated with increased metastatic potential. These proteins were involved in the ‘regulation of mRNA processing’ and ‘cytoskeleton organization’ biological processes. The majority of the proteins were involved in ‘cell proliferation’, ‘migration’ and ‘invasion of cancer’, and may promote HCC metastasis in a synergistic manner. The AKT and nuclear factor-κB signaling pathways may contribute to the regulation of HCC metastasis through regulating the DEPs in SP cells. To the best of our knowledge, the present study is the first to demonstrate the overall proteome difference among SP cells from the different HCC cell lines with different metastatic potentials. The present study provides novel information regarding the metastatic potential of CSCs, which will facilitate further investigation of the topic.

show abstract

MiR-29c inhibits cell growth, invasion, and migration of pancreatic cancer by targeting ITGB1

Cited by 33 publications

References 34 publications

Prognostic microRNAs and their potential molecular mechanism in pancreatic cancer: A study based on The Cancer Genome Atlas and bioinformatics investigation

Prognostic microRNAs and their potential molecular mechanism in pancreatic cancer: A study based on The Cancer Genome Atlas and bioinformatics investigation

microRNA-29c inhibits cell proliferation by targeting NASP in human gastric cancer

Comparative proteomics of side population cells derived from human hepatocellular carcinoma cell lines with varying metastatic potentials

Contact Info

Product

Resources

About